Aggrecan Antibody | 7D4

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Aggrecan Antibody | 7D4 gallery image 1

Recombinant human aggrecan detected with Mouse anti Human Aggrecan (MCA1454G) followed by Goat anti Mouse IgG:Dylight®800. Visualised using a LI-COR Odyssey® infrared imaging system

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Aggrecan Antibody | 7D4 gallery image 2

Published customer image:
Mouse anti Human aggrecan antibody, clone 7D4 used for the detection of aggrecan in bovine synovial explants by immunohistochemistry on formalin fixed, paraffin embedded tissue sections
Image caption:
nfluence of TGF-ß1 on the BMP-2-induced deposition of cartilaginous proteins in synovial explants (immunohistochemical analysis). Light micrographs of sections through bovine explants that had been cultured for 6 weeks under the indicated stimulation conditions and then either stained with Toluidine Blue (1st row), or treated for the immunohistochemical detection of aggrecan (2nd row), type-II collagen (3rd row), type-IX collagen (4th row), type-X collagen (5th row) or COMP (6th row). BMP-2 was applied at a concentration of 200 ng/ml, and TGF-β1 at one of 10 ng/ml. Each agent was introduced at the onset of the 6-week culturing period and was present throughout its course. Native (uncultured) metacarpal-joint cartilage (final vertical column) served as a positive control. Cell nuclei (2nd to 6th rows) were counterstained with haematoxylin. All photomicrographs are represented at the same final magnification. Bar?=?100 μm.

From: Shintani N, Siebenrock KA, Hunziker EB (2013) TGF-β1 Enhances the BMP-2-Induced Chondrogenesis of Bovine Synovial Explants and Arrests Downstream Differentiation at an Early Stage of Hypertrophy.
PLoS ONE 8(1): e53086.

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  • Aggrecan Antibody | 7D4 thumbnail image 1
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  • Mouse anti Human Aggrecan
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    7D4
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1454GC, E, IP, P, WBdatasheet pdfdatasheet pdf0.2 mg
    MCA1454G
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human Aggrecan antibody, clone 7D4 recognizes human aggrecan, a proteoglycan and member of the aggrecan/versican proteoglycan family, which forms a major component of the extracellular matrix (ECM) of both cartilage and the central nervous system (CNS).

      Studies using a panel of core protein-directed antibodies against human aggrecan, revealed the distribution of different aggrecan isoforms within the CNS, and sub-divided the isoforms into clusters 1-5 accordingly. These studies also showed a difference in the relative abundance of these isoforms when comparing brain and cartilage tissues (Virgintino et al. 2009).  

      Mouse anti Human Aggrecan antibody, clone 7D4 recognizes epitopes within the N-terminal G1-IGD-G2 region of aggrecan, that has been identified as a cluster 1 isoform.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Bovineyes
      Chickenno
      Fishno
      Ratno
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Purified human articular cartilage aggrecan
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised Balb/c mice were fused with cells of the mouse NS1 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      hyaluronic acid binding
      cell adhesion
      skeletal system development
      extracellular matrix structural constituent
      sugar binding
      protein binding
      proteolysis
      proteinaceous extracellular matrix
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Immunohistology - Frozen
      Immunohistology - Paraffin
      Immunoprecipitation
      Western Blotting

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
      Cerebral Cortex
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Aggrecan Antibody Formats

    Formats Clone Applications Sizes available
    Aggrecan Antibody : Purified 7D4 C, E, IP, P, WB 0.2 mg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

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      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      STAR9B
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      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
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      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
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      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
      STAR120P
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
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      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
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      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Cerebral Cortex

              References

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