AES Antibody

AES Antibody gallery image 1

Western blot analysis of whole cell lysate from HEK293 human epithelial kidney cells with Rabbit anti Human AES (AHP1006)

AES Antibody gallery image 2

Immunocytochemical staining of HeLa cells with Rabbit anti Human AES (AHP1006)

  • AES Antibody thumbnail image 1
  • AES Antibody thumbnail image 2
  • Rabbit anti AES (C-Terminal)
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP1006C, WBdatasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Rabbit anti Human AES1 (C-terminal) antibody recognizes an epitope within the C-Terminal region (CT) of the 197-amino acid protein AES (amino-Terminal enhancer of split), a member of the Groucho/transducin-like enhancer of split (TLE) family of transcriptional regulators, homologous to the amino-Terminal domain of Drosophila Groucho protein.

      The detachment of integrin from the extracellular matrix (ECM), results in loss of cell adhesion and cell death, termed anoikis. This type of apoptosis is controlled by integrin-mediated signaling pathways and that the mitochondrial protein Bit1 (Bcl-2 inhibitor of transcription), released into the cytoplasm during apoptosis, forms a complex with AES, to induce cell death. The effects of the interaction between Bit1 and AES can be counteracted by the attachment of cells to fibronectin through integrin α5β1.
    • Intended Use
    • Target Species
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to Human AES (CT) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
    • Preservative Stabilisers
      0.02%Sodium Azide (NaN3)
    • Immunogen
      A 16 amino acid peptide located near human AES carboxy-terminus.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      Wnt receptor signaling pathway
      response to interleukin-1
      negative regulation of protein binding
      transcription corepressor activity
      negative regulation of canonical Wnt receptor signaling pathway
      organ morphogenesis
      negative regulation of response to cytokine stimulus
      protein binding
      negative regulation of gene-specific transcription from RNA polymerase II promoter
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen
      Western Blotting1ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      AHP1006 detects a band of approximately 22kDa in human 293 cell lysates
    • Instructions For Use

    Additional AES Antibody Formats

    Formats Applications Sizes available
    AES Antibody : Purified C, WB 0.1 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (Fc):BiotinSTAR121B1 mgE, WB
      Sheep anti Rabbit IgG:Biotin2AB02B1 mlC, E, P, WB
      Sheep anti Rabbit IgG:DyLight®488STAR36D488GA0.1 mgF, IF
      Sheep anti Rabbit IgG:DyLight®549STAR36D549GA0.1 mgF, IF
      Sheep anti Rabbit IgG:DyLight®649STAR36D649GA0.1 mgF, IF
      Sheep anti Rabbit IgG:Dylight®800STAR36D800GA0.1 mgF, IF, WB
      Goat anti Rabbit IgG (Fc):FITCSTAR121F1 mgF
      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      Goat anti Rabbit IgG (H/L):HRPSTAR124P1 mgC, E, WB
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      Sheep anti Rabbit IgG:RPESTAR35A1 mlF

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          TidyBlot Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*

          Recommended Positive Controls

            Histology Controls

              Further Reading

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