Adiponectin

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Adiponectin gallery image 1

Sandwich ELISA analysis of human adiponectin binding using Mouse anti Human adiponectin (MCA4652) as a capture reagent and biotinylated Mouse anti Human adiponectin (MCA2695) as a detection reagent with recombinant human adiponectin (PHP196) as antigen for the generation of the standard curve. Detection is by HRP conjugated streptavidin. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). A plasma sample diluted 1:4 (green) is shown

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Adiponectin gallery image 2

Sandwich ELISA analysis of adiponectin binding using Mouse anti Human adiponectin (MCA4653) as a capture reagent and biotinylated Mouse anti Human adiponectin (MCA2695) as a detection reagent with recombinant human adiponectin (PHP196) as antigen for generation of the standard curve. Detection is by HRP conjugated streptavidin. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). A plasma sample diluted 1:4 (green) is shown

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  • Recombinant Human Adiponectin
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Recombinant Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    PHP196E, FNdatasheet pdfdatasheet pdf25 µg
    PHP196
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Recombinant Adiponectin producted in Hi5 cells derived from the cabbage looper moth Trichoplusia ni corresponds to amino acids 19-244 of the human adiponectin precurson and containing an N-terminal histidine tag. Adiponectin is an adipokine exclusively secreted by adipocytes, which plays a major role in the control of fat metabolism and insulin sensitivity, through the regulation of energy homeostasis and glucose and lipid metabolism. A reduced level of plasma adiponectin is associated with type 2 diabetes and obesity insulin resistance. Adiponectin is an abundant protein in normal serum corresponding to approximately 0.1% of the total protein content.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Functional Assays

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Adiponectin Formats

    Formats Applications Sizes available
    Adiponectin : Rec. Protein E, FN 25 µg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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