Adiponectin Antibody | 5H7

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Adiponectin Antibody | 5H7 gallery image 1

Sandwich ELISA analysis of human adiponectin binding using Mouse anti Human adiponectin (MCA4652) as a capture reagent and biotinylated Mouse anti Human adiponectin (MCA2695) as a detection reagent with recombinant human adiponectin (PHP196) as antigen for the generation of the standard curve. Detection is by HRP conjugated streptavidin. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). A plasma sample diluted 1:4 (green) is shown

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Adiponectin Antibody | 5H7 gallery image 2

Sandwich ELISA analysis of human adiponectin binding using Mouse anti Human adiponectin (MCA4653) as a capture reagent and biotinylated Mouse anti Human adiponectin (MCA2695) as a detection reagent with recombinant human adiponectin (PHP196) as antigen for the generation of the standard curve. Detection is by HRP conjugated streptavidin. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). A plasma sample diluted 1:4 (green) is shown

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  • Mouse anti Human Adiponectin
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  • Product Type
    Monoclonal Antibody
  • Clone
    5H7
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2695E, WBdatasheet pdfdatasheet pdf0.1 mg
    MCA2695
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Mouse anti Human adiponectin, clone 5H7 recognizes the collagen-like domain of human adiponectin, also known as Acrp30, Gelatin-binding protein, Adipose most abundant gene transcript 1 protein, Adipocyte complement-related 30 kDa protein or apM-1. Adiponectin a 244 amino acid ~30kDa major adipokine secreted into the bloodstream from adipose tissue into the circulation where it exists as three distinct, low, medium and high molecular weight oligomeric forms (UniProt: Q15848).

      Circulating adiponectin levels are correlated with insulin sensitivity and decreased levels parallel the change in insulin sensitivity during the progression to type II diabetes (Heilbronn et al. 2011). Mutations in the adiponectin gene can also lead to adiponectin deficiency charactrized by obesity, diabetes, high blood pressure and circulating cholerterol (Takahashi et al. 2000).

      Mouse anti Human adiponectin, clone 5H7 had been used succesfully as a detection reagent in the development of sensitive sandwich ELISA's in conjunction with Mouse anti adiponectin clones Adn27 or Adn36 as capture reagents.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Western Blotting1/10001/2000

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      MCA2695 detects a band of approximately 64kDa in mouse liver cell lysates.
    • Instructions For Use

    Additional Adiponectin Antibody Formats

    Formats Clone Applications Sizes available
    Adiponectin Antibody : Purified 5H7 E, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Further Reading

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