ADHFE1 Antibody (PrecisionAbTM Antibody)

//www.bio-rad-antibodies.com/human-adhfe1-antibody-vpa00299.html?RenderPageSpecific=1
ADHFE1 Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with ADHFE1 antibody followed by detection with HRP conjugated Goat anti Rabbit (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 62 second exposure. Arrow points to ADHFE1 (molecular weight 50 kDa)

Enlarge
  • ADHFE1 Antibody (PrecisionAb<sup>TM</sup> Antibody) thumbnail image 1
  • Rabbit anti ADHFE1
(Rated 0.0 out of 5 based on 0 customer reviews)
    Rabbit anti human ADHFE1 antibody recognizes hydroxyacid-oxoacid transhydrogenase, mitochondrial, also known as Fe-containing alcohol dehydrogenase 1, alcohol dehydrogenase 8 or alcohol dehydrogenase iron-containing protein 1. This antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Polyclonal
    • Isotype
      Polyclonal IgG
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VPA00299WBdatasheet pdfdatasheet pdf100 µl
      VPA00299
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
      -
      • Rabbit anti Human ADHFE1 antibody recognizes ADHFE1 also known as iron-containing alcohol dehydrogenase 1, alcohol dehydrogenase 8 and hydroxyacid-oxoacid transhydrogenase, mitochondrial.

        The ADHFE1 gene encodes hydroxyacid-oxoacid transhydrogenase (EC 1.1.99.24), which is responsible for the oxidation of 4-hydroxybutyrate in mammalian tissues (Kardon et al. 2006, PubMed 16616524, OMIM, Mar 2008).

        Rabbit anti Human ADHFE1 antibody detects a band of 50 kDa. The antibody has been extensively validated for western blotting using human tissue and whole cell lysates.
      • Target Species
        Human
      • Western Blotting
        Anti ADHFE1 detects a band of approximately 50 kDa in human liver tissue
      • Product Form
        Purified IgG - liquid
      • Preparation
        Rabbit polyclonal antibody purified by affinity chromatography
      • Buffer Solution
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
        2% Sucrose
      • Immunogen
        Synthetic peptide corresponding to the central region of human ADHFE1
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
      • Lysate Reconstitution
      • Storage
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles
      • Shelf Life
        As supplied, 12 months from date of despatch
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories
      • Regulatory
        For research purposes only
      • GO Terms
        molecular hydrogen transport
        mitochondrial matrix
        2-oxoglutarate metabolic process
        oxidation-reduction process
        hydroxyacid-oxoacid transhydrogenase activity
        metal ion binding
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2016 Bio-Rad

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Rabbit IgG (H/L):HRPSTAR208P2 mlWB
        STAR208P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

                Write your review

                Western Blotting without Trial and Error: Use PrecisionAb™ Antibodies

                Watch how PrecisionAb Validated Western Blotting Antibodies are defining a new standard of quality that you can trust to deliver superior results and reproducibility in western blotting.

                Contact Information


                Online Payment Methods:

                1. Invoice / Purchase Order
                2. Credit Card (Maestro, VISA,MasterCard & VISA Electron)
                credit cards accepted
                • View more products with "ADHFE1" specificity.