Actin Antibody | AbD22606

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hFAB Rhodamine Housekeeping Protein Fluorescent Primary Antibodies

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hFAB Rhodamine Housekeeping Protein Fluorescent Primary Antibodies in Multiplex Fluorescent Western Blotting. Simultaneous detection of multiple proteins (ATG7, AKR1C2, and tubulin) on a single blot using fluorescent antibodies

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  • ANTI-ACTIN hFAB™ Rhodamine Antibody
  • ANTI-ACTIN hFAB™ Rhodamine Antibody
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  • Product Type
    Monoclonal Antibody
  • Clone
    AbD22606
  • Isotype
    HuCAL Fab monovalent
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    12004163WBdatasheet pdfdatasheet pdf200 µl
    12004163
    12004164WBdatasheet pdfdatasheet pdf40 µl
    12004164
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • hFAB Rhodamine Housekeeping Protein Fluorescent Primary Antibodies allow one-step detection of common housekeeping proteins like actin, tubulin, and GAPDH in your samples. These anti-actin, anti-tubulin, and anti-GAPDH antibodies are directly labeled with a rhodamine derivative (Exmax/Emmax = 530 nm/580 nm). The hFAB Rhodamine Antibodies were generated with Human Combinatorial Antibody Library (HuCAL®) Technology, providing exceptional specificity.

      These housekeeping protein antibodies can be used for protein normalization when the protein loading amounts are in the linear dynamic range. hFAB Rhodamine Antibodies can be imaged with the ChemiDoc™ MP Imaging System, and can be used in multiplex experiments with StarBright™ Blue 700 Secondary Antibodies as well as other traditional fluorescent antibodies, permitting detection of multiple target proteins and the housekeeping protein in a single blot.

      Features and Benefits

      Simple — No requirement for a secondary antibody; one-step detection of housekeeping proteins using highly cross-adsorbed antibodies for high specificity and sensitivity
      Versatile — Compatible with use of a wide range of other fluorophores including StarBright Blue 700 and DyLight 800 Fluorophores for multiplex western blotting
      Unique — HuCAL Technology eliminates cross-reactivity with primary/secondary antibodies from other species
      Diverse — Antibodies have been tested to detect human or mouse housekeeping proteins
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Mouseyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      A monovalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. and purified using NiNTA affinity chromatography. This antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain and conjugated to rhodamine - lyophilized
      A monovalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. and purified using NiNTA affinity chromatography. This antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain and conjugated to rhodamine - lyophilized
    • Reconstitution
      Resuspend contents of the tube in the indicated volume of distilled or deionized water and leave on ice for at least 30 min prior to use. Brief centrufugation (pulse spin for 2-3 sec at maximum speed on a tabletop microcentrifuge) may be used to collect the contents at the bottom of the tube.
      Resuspend contents of the tube in the indicated volume of distilled or deionized water and leave on ice for at least 30 min prior to use. Brief centrufugation (pulse spin for 2-3 sec at maximum speed on a tabletop microcentrifuge) may be used to collect the contents at the bottom of the tube.
    • Preparation
    • Preservative Stabilisers
      >80% Sucrose
      <0.04% 2-Methyl-2H-Isothiazol-3-One
      <10% Bovine Serum Albumin
      >80% Sucrose
      <0.04% 2-Methyl-2H-Isothiazol-3-One
      <10% Bovine Serum Albumin
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      1 year at -20oC lyophilized; 6 months at +4oC after resuspension.
      This product is photosensitive and should be protected from light
      DO NOT FREEZE the solubilized material
      1 year at -20oC lyophilized; 6 months at +4oC after resuspension.
      This product is photosensitive and should be protected from light
      DO NOT FREEZE the solubilized material
    • Shelf Life
      Please see label for expiry date
      Please see label for expiry date
    • UniProt
    • Entrez Gene
    • Acknowledgements
      Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
      His-tag is a registered trademark of EMD Biosciences.
      Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
      His-tag is a registered trademark of EMD Biosciences.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/1,0001/10,000
    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/1,0001/10,000

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
      Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/12004163.pdf
    • Instructions For Use
      Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/12004163.pdf

    Additional Actin Antibody Formats

    Formats Clone Applications Sizes available
    Actin Antibody : Rhodamine AbD22606 WB 200 µl | 40 µl
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
          STAR117D800GA
          Sheep anti Rabbit IgG:Dylight®800STAR36D800GA0.1 mgF, IF, WB
          STAR36D800GA
          ANTI-TUBULIN hFAB™ Rhodamine Antibody12004165200 µlWB
          12004165
          ANTI-GAPDH hFAB™ Rhodamine Antibody12004167200 µlWB
          12004167
          ANTI-TUBULIN hFAB™ Rhodamine Antibody1200416640 µlWB
          12004166
          ANTI-GAPDH hFAB™ Rhodamine Antibody1200416840 µlWB
          12004168
          Goat ANTI-MOUSE IgG StarBright™ Blue 70012004158400 µlWB
          12004158
          Goat ANTI-RABBIT IgG StarBright™ Blue 70012004161400 µlWB
          12004161
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
          STAR117D800GA
          Sheep anti Rabbit IgG:Dylight®800STAR36D800GA0.1 mgF, IF, WB
          STAR36D800GA
          ANTI-TUBULIN hFAB™ Rhodamine Antibody12004165200 µlWB
          12004165
          ANTI-GAPDH hFAB™ Rhodamine Antibody12004167200 µlWB
          12004167
          ANTI-TUBULIN hFAB™ Rhodamine Antibody1200416640 µlWB
          12004166
          ANTI-GAPDH hFAB™ Rhodamine Antibody1200416840 µlWB
          12004168
          Goat ANTI-MOUSE IgG StarBright™ Blue 70012004158400 µlWB
          12004158
          Goat ANTI-RABBIT IgG StarBright™ Blue 70012004161400 µlWB
          12004161

          Recommended Positive Controls

            Histology Controls

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