Actin Alpha Antibody | 1A4

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Actin Alpha Antibody | 1A4 gallery image 1

Published customer image:
Mouse anti Human alpha actin antibody, clone 1A4 used to demonstrate actin isoform expression in adult rat skeletal muscle by immunofluorescence.
Image caption:
Regenerating rat adult skeletal muscle co-stained with isoform specific antibodies.
C) At 4d–5d post-injury, co-staining with anti-α-SMA (red) and anti-α-CAA (green) shows that during the healing process, only a few fibers co-express both isoforms after 4d (a–c, arrowhead), whereas after 5d, only α-CAA positive fibers are detected (d–f). After 4d, myofibroblasts might participate to the repair process and are detected by using the anti-α- SMA mAb (a–c, arrow). Merged images are shown on right column. Bars = 50μm.

From: Chaponnier C, Gabbiani G.
Monoclonal antibodies against muscle actin isoforms: epitope identification and analysis of isoform expression by immunoblot and immunostaining in normal and regenerating skeletal muscle.
F1000Res. 2016, 5: 416.

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  • Actin Alpha Antibody | 1A4 thumbnail image 1
  • Mouse anti Human Actin Alpha (Smooth Muscle)
(Rated 5.0 out of 5 based on 1 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    1A4
  • Isotype
    IgG2a
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA5781GAC, E, IF, P*, WBdatasheet pdfdatasheet pdf0.1 mg
    MCA5781GA
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti human actin alpha (smooth muscle) antibody, clone 1A4 recognises alpha smooth muscle actin. Actins are highly conserved proteins that play a role in cell contractile activity, motility, cytokinesis, and in maintaining cell structure. Alpha smooth muscle actin is expressed in vascular smooth muscle, myofibroblasts and myoepithelial cells and also in tumours derived from these cells.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Pigyes
      Rabbityes
      Ratyes
      Mouseyes
      Sheepyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Acetylated N-terminal synthetic decapeptide of alpha smooth muscle actin coupled to KLH.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Immunofluorescence
      Immunohistology - Frozen1/501/100
      Western Blotting
      Immunohistology - Paraffin(1)
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
      Smooth muscle in vessels
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Actin Alpha Antibody Formats

    Formats Clone Applications Sizes available
    Actin Alpha Antibody : Purified 1A4 C, E, IF, P*, WB 0.1 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

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      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
      STAR120F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
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      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Smooth muscle in vessels

              References

              Write your review

              Excellent for immunofluorescence in rat cells
              5/5stars
              by
              Submitted: 8 Feb 2017
              We previously had used the 1A4 clone from another major supplier who recently lost their license to sell antibodies. Looking for a replacement, we tried Bio-Rad's Actin Alpha Antibody 1A4 primary. We compared dilutions of both antibodies side-by-side and found equivalent staining of both, which appear to be highly specific for actin fibres in A7r5 rat aorta smooth muscle cells. We diluted the primary to 200 microg/ml. Using 4% normal goat serum as block, we get excellent staining at 300-500x dilution (0.4-0.67 microg/ml). One could probably go to 0.1-0.2 microg/ml if using a high NA objective and sensitive imaging system. We will post images soon.

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