ABCC4 Antibody (PrecisionAbTM Antibody)

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ABCC4 Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with ABCC4 antibody followed by detection with HRP conjugated Donkey anti Sheep/Goat IgG (1/10,000, STAR88P) and visualized on the ChemiDoc MP with 69 second exposure. Arrow points to ABCC4 (molecular weight 200 kDa)

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  • ABCC4 Antibody (PrecisionAb<sup>TM</sup> Antibody) thumbnail image 1
  • Goat anti ABCC4
  • ABCC4 Antibody with Control Lysate
  • ABCC4 Antibody with Control Lysate
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    ABCC4 antibody recognizes multidrug resistance-associated protein 4, also known as ATP-binding cassette sub-family C member 4, MRP/cMOAT-related ABC transporter, multi-specific organic anion transporter B or MOAT-B. The antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Polyclonal
    • Isotype
      Polyclonal IgG
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VPA00068WBdatasheet pdfdatasheet pdf100 µl
      VPA00068
      VPA00068KWBdatasheet pdfdatasheet pdf datasheet pdf10 Westerns
      VPA00068K
      VPA00068KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VPA00068KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
      -
      • Goat anti Human ABCC4 antibody recognizes ABCC4 a 200 kDa protein and a member of the MRP family of transporters.

        The ABCC4 protein shows a broad pattern of expression having been detected in the kidneys, liver, erythrocytes, adrenal glands, platelets, brain, and pancreas. ABCC4 is present in the apical membrane as well as in the basolateral membrane and is capable of transporting a broad number of substrates, including bile salt conjugates, conjugated steroids, cyclic nucleotides, nucleoside analogs, eicosanoids, and cardiovascular drugs. Although ABCC4 is present in many human drug-resistant cancer cell lines, it remains to be determined if ABCC4 is associated with resistance to drugs in solid tumors (Borst et al. 2007).

        Goat anti Human ABCC4 antibody detects ABCC4 as a band of 200 kDa in a range of human cell line lysates.
      • Target Species
        Human
      • Western Blotting
        Goat anti ABCC4 detects a band of approximately 200 kDa in K562 cell lysates.
        Goat anti ABCC4 detects a band of approximately 200 kDa in K562 cell lysates.
        Goat anti ABCC4 detects a band of approximately 200 kDa in K562 cell lysates.
      • Product Form
        Purified IgG - liquid
        Purified IgG - liquid
        Purified IgG - liquid
      • Preparation
        Goat polyclonal antibody purified by affinity chromatography
        Pack Size: 10 Westerns100μl Goat polyclonal antibody purified by affinity chromatography
        Pack Size: 2 Westerns20μl Goat polyclonal antibody purified by affinity chromatography
        Pack Size: 10 Westerns100μl Goat polyclonal antibody purified by affinity chromatography
        Pack Size: 2 Westerns20μl Goat polyclonal antibody purified by affinity chromatography
      • Buffer Solution
        TRIS buffered saline
        TRIS buffered saline
        TRIS buffered saline
      • Preservative Stabilisers
        0.02% Sodium Azide (NaN3)
        0.5% Bovine Serum Albumin
        0.02% Sodium Azide (NaN3)
        0.5% Bovine Serum Albumin
        0.02% Sodium Azide (NaN3)
        0.5% Bovine Serum Albumin
      • Immunogen
        Synthetic peptide with CNGQPSTLTIFETAL from the C terminus of the protein sequence.
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg K562 lysate lyophilized in RIPA buffer.
        400μg K562 lysate lyophilized in RIPA buffer.
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
      • Shelf Life
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      • Regulatory
        For research purposes only
      • GO Terms
        plasma membrane
        15-hydroxyprostaglandin dehydrogenase (NAD+) activity
        platelet dense granule membrane
        ATP binding
        chloride channel activity
        membrane fraction
        platelet degranulation
        platelet activation
        integral to membrane
        ATPase activity, coupled to transmembrane movement of ions, phosphorylative mechanism
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2016 Bio-Rad

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Donkey anti Sheep/Goat IgG:HRPSTAR88P1 mlC, E, P, WB
        STAR88P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Donkey anti Sheep/Goat IgG:HRPSTAR88P1 mlC, E, P, WB
        STAR88P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Donkey anti Sheep/Goat IgG:HRPSTAR88P1 mlC, E, P, WB
        STAR88P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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