A-FABP Antibody (PrecisionAbTM Antibody)

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A-FABP Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with A-FABP antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/50,000, STAR207P) and visualized on the ChemiDoc MP with 31.4 second exposure. Arrow points to A-FABP (molecular weight 15 kDa)

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  • A-FABP Antibody (PrecisionAb<sup>TM</sup> Antibody) thumbnail image 1
  • Mouse anti A-FABP
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    A-FABP antibody recognizes fatty acid-binding protein, adipocyte, also known as adipocyte lipid-binding protein, adipocyte-type fatty acid-binding protein, epididymis secretory protein Li 104 or fatty acid-binding protein 4. This antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Monoclonal
    • Isotype
      IgG1
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VMA00343WBdatasheet pdfdatasheet pdf100 µl
      VMA00343
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
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      • Mouse anti Human A-FABP antibody recognizes the fatty acid-binding protein, adipocyte, also known as adipocyte lipid-binding protein, adipocyte-type fatty acid-binding protein, epididymis secretory protein Li 104 and fatty acid-binding protein 4.

        Encoded by the FABP4 gene, A-FABP is fatty acid binding protein found in adipocytes. Fatty acid binding proteins are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. It is thought that FABPs roles include fatty acid uptake, transport, and metabolism (provided by RefSeq, Jul 2008).

        Mouse anti Human A-FABP antibody detects a band of 14 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
      • Target Species
        Human
      • Western Blotting
        Anti A-FABP detects a band of approximately 15 kDa in human heart tissue lysate
      • Product Form
        Purified IgG - liquid
      • Preparation
        Mouse monoclonal antibody prepared by affinity chromatography on Protein G
      • Buffer Solution
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
      • Immunogen
        Recombinant protein corresponding to amino acids 1-132 of human A-FABP expressed in E. coli
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
      • Lysate Reconstitution
      • Storage
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
      • Shelf Life
        As supplied, 12 months from date of despatch.
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      • Regulatory
        For research purposes only
      • GO Terms
        nucleus
        transporter activity
        fatty acid binding
        triglyceride catabolic process
        soluble fraction
        protein binding
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2016 Bio-Rad

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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