14-3-3 Sigma Antibody (PrecisionAbTM Antibody)

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14-3-3 Sigma Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with 14-3-3 sigma antibody followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 1 second exposure. Arrow points to 14-3-3 sigma (molecular weight 27 kDa)

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  • 14-3-3 Sigma Antibody (PrecisionAb<sup>TM</sup> Antibody) thumbnail image 1
  • 14-3-3 Sigma Antibody with Control Lysate
  • Rabbit anti 14-3-3 Sigma
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  • Product Type
    PrecisionAb™ Polyclonal
  • Isotype
    Polyclonal IgG
  • Format
    Purified
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    VPA00717WBdatasheet pdfdatasheet pdf100 µl
    VPA00717
    VPA00717KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
    VPA00717KT
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    References
    Reviews
    -
    • Rabbit anti Human 14-3-3 sigma antibody recognizes the protein 14-3-3 sigma, also known as epithelial cell marker protein 1 or stratifin.

      Hypermethylation of stratifin has been linked to early stages in breast cancer. Additionally, it has been shown to promote MMP-1 expression in fibroblasts.

      Rabbit anti Human 14-3-3 sigma antibody detects a band of 27 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. This antibody is available with a positive control lysate, in a smaller trial size, and as a standalone antibody.
    • Target Species
      Human
    • Western Blotting
      Rabbit anti 14-3-3 sigma detects a band of approximately 27 kDa in A431 cell lysates
      Rabbit anti 14-3-3 sigma detects a band of approximately 27 kDa in A431 cell lysates
    • Product Form
      Purified IgG - liquid
      Purified IgG - liquid
    • Preparation
      20μl Rabbit polyclonal antibody purified by affinity chromatography
      Rabbit polyclonal antibody purified by affinity chromatography
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Preservative Stabilisers
      0.09% Sodium Azide
      0.09% Sodium Azide
    • Immunogen
      KLH conjugated synthetic peptide of human 14-3-3 sigma (amino acids 222 - 248)
    • Instructions for Use
      Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
    • Lysate Composition
      400μg A431 lysate lyophilized in RIPA buffer.
    • Lysate Reconstitution
      - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
      - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
      Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
    • Storage
      Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

      Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
      Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
    • Shelf Life
      As supplied, 12 months from date of despatch
      As supplied, 12 months from date of despatch.
    • Acknowledgements
      PrecisionAb™ is a trademark of Bio-Rad Laboratories
      PrecisionAb™ is a trademark of Bio-Rad Laboratories.
    • Regulatory
      For research purposes only
    • GO Terms
      protein kinase C inhibitor activity
      release of cytochrome c from mitochondria
      DNA damage response, signal transduction resulting in induction of apoptosis
      extracellular space
      negative regulation of caspase activity
      protein domain specific binding
      cytoplasm
      nucleus
    • UniProt
    • Entrez Gene
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/1000
    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/1000

    • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (H/L):HRPSTAR208P2 mlWB
      STAR208P
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (H/L):HRPSTAR208P2 mlWB
      STAR208P

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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