HCA048 is a recombinant antibody with specificity for Green Fluorescent Protein. It has no known reactivity with mammalian proteins or other antigens. It is therefore recommended as a negative control reagent when using other HuCAL antibodies of the same format.
It is recommended that this reagent is used at the same concentration as the test reagent.
A bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library. Expressed in E. coli and purified using purified using NiNTA affinity chromatography. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with myc-tag (EQKLISEEDL) and a Strep-tag at the C-terminus of the antibody heavy chain.
Reconstitute with 1.0ml distilled water
Affinity chromatography on a Strep-tag affinity column.
0.09%Sodium Azide (NaN3) 1% Bovine Serum Albumin
Green fluorescent protein
Approx. Protein Concentrations
Antibody concentration 0.1mg/ml following reconstitution
Reagents In The Kit
Preparing The Antibody
Phosphate buffered saline
Store at -20oC only. Storage in frost-free freezers is not recommended. This product should be stored undiluted.
6 months from date of reconstitution.
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Immunohistology - Frozen
Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.