HuCAL Fab-dHLX-FS Negative Control Antibody

//www.bio-rad-antibodies.com/hucal-fab-dhlx-fs-negative-control-hca047.html?RenderPageSpecific=1
HuCAL Fab-dHLX-FS Negative Control Antibody gallery image 1

Purchased this product? Why not be the first to submit a review and upload an image?

Enlarge
  • HuCAL Fab-dHLX-FS Negative Control Antibody thumbnail image 1
  • HuCAL Fab-dHLX-FS Negative Control
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Negative/Isotype Control
  • Isotype
    HuCAL Fab bivalent
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    HCA047C, E, P, WBdatasheet pdfdatasheet pdf0.1 mg
    HCA047
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • HCA047 is a recombinant antibody with specificity for Green Fluorescent Protein. It has no known reactivity with mammalian proteins or other antigens. It is therefore recommended as a negative control reagent when using other HuCAL antibodies of the same format.

      It is recommended that this reagent is used at the same concentration as the test reagent.
    • Intended Use
    • Target Species
      Negative Control
    • Product Form
      A bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library. Expressed in E. coli and purified using purified using NiNTA affinity chromatography. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a DYKDDDDK tag and a Strep-tag at the C-terminus of the antibody heavy chain.
    • Reconstitution
      Reconstitute with 1.0ml distilled water
    • Preparation
      Affinity chromatography on a Strep-tag affinity column.
    • Preservative Stabilisers
      0.09%Sodium Azide (NaN3)
      1% Bovine Serum Albumin
    • Immunogen
      Green fluorescent protein
    • Purity
    • Approx. Protein Concentrations
      Antibody concentration 0.1mg/ml following reconstitution
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted.
    • Shelf Life
      6 months from date of reconstitution.
    • Acknowledgements
      Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
    • Licensed Use
      For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Immunohistology - Frozen
      Immunohistology - Paraffin
      Western Blotting

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional HuCAL Fab-dHLX-FS Negative Control Antibody Formats

    Formats Applications Sizes available
    HuCAL Fab-dHLX-FS Negative Control Antibody : Purified C, E, P, WB 0.1 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Human IgG F(ab')2:HRP0500-00990.5 mlE, WB
      0500-0099
      Mouse anti Strep-Tag Classic:HRPMCA2489P75 µlE, WB
      MCA2489P

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Write your review

              You may also be interested in...