HuCAL Fab-Cys3H Negative Control Antibody | AbD14892

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  • HuCAL Fab-Cys3H Negative Control
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  • Product Type
    Negative/Isotype Control
  • Clone
    AbD14892
  • Isotype
    HuCAL Fab monovalent
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    HCA260Edatasheet pdfdatasheet pdf0.1 mg
    HCA260
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • HuCAL Fab-Cys3-H Negative Control, clone AbD14892, is a recombinant antibody with specificity for Green Fluorescent Protein. It has no known reactivity with mammalian proteins or other antigens. It is therefore recommended as a negative control reagent when using other HuCAL antibodies of the same format.

      It is recommended that this reagent is used at the same concentration as the test reagent.
    • Intended Use
    • Target Species
      Negative Control
    • Product Form
      A monovalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. The antibody is tagged with a Cys3 tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain.
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
      0.01% Thiomersal
    • Immunogen
      Green Fluorescent Protein
    • Purity
    • Approx. Protein Concentrations
      Antibody concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
    • Shelf Life
      12 months from date of despatch.
    • Acknowledgements
      Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents.
    • Licensed Use
      For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional HuCAL Fab-Cys3H Negative Control Antibody Formats

    Formats Clone Applications Sizes available
    HuCAL Fab-Cys3H Negative Control Antibody : Purified AbD14892 E 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse anti Histidine Tag:Alk.Phos.MCA1396A0.1 mgE, WB
          MCA1396A
          Mouse anti Histidine Tag:BiotinMCA1396B0.1 mgE, WB
          MCA1396B
          Mouse anti Penta Histidine Tag:HRPMCA5995P125 µlE, WB
          MCA5995P
          Mouse anti Histidine TagMCA1396GA0.1 mgE, IP, WB
          MCA1396GA

          Recommended Positive Controls

            Histology Controls

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