HuCAL Fab-A-V5H Negative Control Antibody | AbD23509

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  • HuCAL Fab-A-V5H Negative Control
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  • Product Type
    Negative/Isotype Control
  • Clone
    AbD23509
  • Isotype
    HuCAL Fab bivalent
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    HCA258Edatasheet pdfdatasheet pdf0.1 mg
    HCA258
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • HuCAL Fab-A-V5H Negative Control, clone AbD23509, is a recombinant antibody with specificity for Green Fluorescent Protein. It has no known reactivity with mammalian proteins or other antigens. It is therefore recommended as a negative control reagent when using other HuCAL antibodies of the same format.

      It is recommended that this reagent is used at the same concentration as the test reagent.
    • Intended Use
    • Target Species
      Negative Control
    • Product Form
      A bivalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is bivalent by dimerization of the bacterial alkaline phosphatase fusion protein. The antibody is tagged with a V5 tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain.
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
      0.01% Thiomersal
    • Immunogen
      Green Fluorescent Protein
    • Purity
    • Approx. Protein Concentrations
      Antibody concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
    • Shelf Life
      12 months from date of despatch.
    • Acknowledgements
      Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
    • Licensed Use
      For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional HuCAL Fab-A-V5H Negative Control Antibody Formats

    Formats Clone Applications Sizes available
    HuCAL Fab-A-V5H Negative Control Antibody : Purified AbD23509 E 0.1 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse anti V5-Tag:Alk. Phos.MCA1360A0.1 mgE, WB
          MCA1360A
          Mouse anti Histidine Tag:Alk.Phos.MCA1396A0.1 mgE, WB
          MCA1396A
          Mouse anti V5-Tag:BiotinMCA1360B0.1 mgC, E, WB
          MCA1360B
          Mouse anti Histidine Tag:BiotinMCA1396B0.1 mgE, WB
          MCA1396B
          Mouse anti V5-Tag:HRPMCA1360P0.1 mgE, WB
          MCA1360P
          Mouse anti Penta Histidine Tag:HRPMCA5995P125 µlE, WB
          MCA5995P
          Mouse anti V5-TagMCA1360GA0.1 mgC, E, F, IF, IP, R, WB
          MCA1360GA
          Mouse anti Histidine TagMCA1396GA0.1 mgE, IP, WB
          MCA1396GA

          Recommended Positive Controls

            Histology Controls

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