MHC Class I Monomorphic Antibody | CVS22

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MHC Class I Monomorphic Antibody | CVS22 gallery image 1

Staining of equine peripheral blood lymphocytes with Mouse anti Equine MHC Class 1 (MCA1086GA), followed by Goat anti Mouse IgG:FITC (STAR117F)

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MHC Class I Monomorphic Antibody | CVS22 gallery image 2

Staining of equine peripheral blood lymphocytes with Mouse anti Equine MHC Class 1:FITC (MCA1086F)

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MHC Class I Monomorphic Antibody | CVS22 gallery image 3

Staining of equine peripheral blood lymphocytes with Mouse anti Equine MHC Class 1:RPE (MCA1086PE)

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MHC Class I Monomorphic Antibody | CVS22 gallery image 4

Published customer image:
Mouse anti Horse MHC class I antibody, clone CVS22 used for evaluation of MHC class I expressing white blood cells in equine samples by flow cytometry.
Image caption:
Antigen expression on WBC in CB, and CB-MSC at passage (p) 2, 3 and 5. Consistent gates were applied for all cell analyses. Control sample fluorescence is indicated by black lines, fluorescence of samples incubated with specific antibody by light blue/gray lines. Results were similar for samples from 5 animals. Numbers in each plot represent the mean proportion of cells expressing each antigen.

From: Tessier L, Bienzle D, Williams LB, Koch TG (2015) Phenotypic and Immunomodulatory Properties of Equine Cord Blood-Derived Mesenchymal Stromal Cells. PLoS ONE 10(4): e0122954.

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  • Mouse anti Horse MHC Class I Monomorphic
  • Mouse anti Horse MHC Class I Monomorphic:RPE
  • Mouse anti Horse MHC Class I Monomorphic:FITC
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  • Product Type
    Monoclonal Antibody
  • Clone
    CVS22
  • Isotype
    IgG2a
3 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1086GAC, F, IPdatasheet pdfdatasheet pdf0.1 mg
    MCA1086GA
    MCA1086FFdatasheet pdfdatasheet pdf0.1 mg
    MCA1086F
    MCA1086PEFdatasheet pdfdatasheet pdf100 Tests
    MCA1086PE
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Mouse anti Horse MHC Class I Monomorphic antibody, clone CVS22 recognizes monomorphic equine MHC Class I and was classified at the International Equine Leucocyte Antigen Workshop. MHC class I is expressed by all nucleated cells.

      The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In horses, this complex is referred to as the equine leukocyte antigen (ELA) region.
    • Intended Use
    • Target Species
      Horse
    • Product Form
      Purified IgG - liquid
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
    • Reconstitution
      Reconstitute with 1.0 ml distilled water
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      5% Sucrose
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
    • Immunogen
      Equine leucocytes
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
      IgG concentration 0.1 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline.
      Phosphate buffered saline
      Phosphate buffered saline.
    • Fusion Partners
      Spleen cells from immunised mice were fused with cells of the X63.Ag 8.653 mouse myeloma cell line.
    • Storage
      Store at +4oC for one month or at -20oC for longer.

      This product should be stored undiluted.
      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC.
      After reconstitution store at +4oC.
      DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light.
      Store at +4oC for one month or at -20oC for longer.

      This product should be stored undiluted.
      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      12 months from date of reconstitution.
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/251/200
      Immunohistology - Frozen
      Immunoprecipitation
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional MHC Class I Monomorphic Antibody Formats

    Formats Clone Applications Sizes available
    MHC Class I Monomorphic Antibody : FITC CVS22 F 0.1 mg
    MHC Class I Monomorphic Antibody : Purified CVS22 C, F, IP 0.1 mg
    MHC Class I Monomorphic Antibody : RPE CVS22 F 100 Tests
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
      STAR117A
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      STAR87A
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
      STAR117D488GA
      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF
      STAR117D549GA
      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
      STAR117D649GA
      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
      STAR117D680GA
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      STAR117D800GA
      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      STAR8D800GA
      Human anti Mouse IgG2a:FITCHCA037F0.1 mgF
      HCA037F
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      STAR117F
      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
      STAR70
      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
      STAR120F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      STAR9B
      Human anti Mouse IgG2a:HRPHCA037P0.1 mgE
      HCA037P
      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      STAR117P
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      STAR77
      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
      STAR120P
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
      STAR13B
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      STAR87P
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      STAR12A
      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
      STAR76

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse anti Horse MHC Class II Monomorphic:FITCMCA1085F0.1 mgF
          MCA1085F
          Mouse anti Horse MHC Class II MonomorphicMCA1085GA0.1 mgC, F, IP
          MCA1085GA

          Recommended Positive Controls

            Histology Controls

              References

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