Histar Detection Kit

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Histar Detection Kit gallery image 1

Detection of Mouse anti Troponin T carciac isoform 1 on formalin fixed, paraffin embedded human heart tissue using the Histar detection system (STAR3000A)

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Histar Detection Kit gallery image 2

Detection of Mouse anti strep-tag-immo (MCA2488) in an immunohistochemical analysis of strep tagged Human anti Human CD276 (HCA143) on formalin fixed, paraffin embedded prostate chippings following heat mediated antigen retrieval with citrate buffer, pH6.0 using the Histar detection system (STAR3000A)

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Histar Detection Kit gallery image 3

Detection of Mouse anti strep-tag-immo (MCA2488) in an immunohistochemical analysis of strep tagged Human anti Human CD127 (HCA147) on acetone fixed human tonsil using the Histar detection kit (STAR3000A)

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Histar Detection Kit gallery image 4

Detection of Mouse anti strep-tag-immo (MCA2488) in an immunohistochemical analysis of strep tagged Human anti Human PINK1 (HCA150) on formalin fixed, paraffin embedded human testis following heat mediated antigen retrieval with citrate buffer, pH6.0 using the Histar detection kit (STAR3000A)

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Histar Detection Kit gallery image 5

Detection of Mouse anti Human estrogen receptor (MCA1974) in an immunohistochemical analysis of estrogen receptor expression on formalin fixed, paraffin embedded human placenta following heat mediated antigen retrieval with citrate buffer, pH6.0 using the Histar detection kit (STAR300A)

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Histar Detection Kit gallery image 6

Detection of Mouse anti Human estrogen receptor (MCA1974) in an immunohistochemical analysis of estrogen receptor expression on formalin fixed, paraffin embedded human breast cancer tissue folowing heat mediated antigen retrieval with EDTA, pH8.0 using the Histar detection kit (STAR3000A)

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Histar Detection Kit gallery image 7

Detection of Mouse anti Human chromogranin A (MCA5696) in an immunohistochemical analysis of formalin fixed, paraffin embedded human pancreas following heat mediated antigen retrieval with citrate buffer, pH6.0 using the Histar detection kit (STAR3000A)

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Histar Detection Kit gallery image 8

Detection of Mouse anti Human prostate specific antigen (MCA5842G) in an immunohistochemical analysis of PSA expression on formalin fixed, paraffin embedded human prostate using the Histar detection kit (STAR3000A), original magnification x100

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Histar Detection Kit gallery image 9

Detection of Mouse anti Human prostate specific antigen (MCA5842G) in an immunohistochemical analysis of PSA expression on formalin fixed, paraffin embedded human prostate using the Histar detection kit (STAR3000A), original magnification x200

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Histar Detection Kit gallery image 10

Detection of Mouse anti Human prostate specific antigen (MCA5843G) in an immunohistochemical analysis of PSA expression on formalin fixed, paraffin embedded human prostate using the Histar detection kit (STAR3000A), original magnification x100

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Histar Detection Kit gallery image 11

Detection of Mouse anti Human prostate specific antigen (MCA5843G) in an immunohistochemical analysis of PSA expression on formalin fixed, paraffin embedded human prostate using the Histar detection kit (STAR3000A), original magnification x200

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Histar Detection Kit gallery image 12

Immunoperoxidase staining of human spleen cryosection with Mouse anti Human CD11b antibody, clone ICRF44 (MCA551) followed by the Histar detection system (STAR3000). High power

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  • Histar Detection System
  • Histar Detection System
  • Histar Detection System
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    1 Formats Available
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      STAR3000BC, Pdatasheet pdfdatasheet pdf datasheet pdf datasheet pdf150 Tests
      STAR3000B
      STAR3000AC, Pdatasheet pdfdatasheet pdf datasheet pdf datasheet pdf datasheet pdf datasheet pdf datasheet pdf50 Tests
      STAR3000A
      STAR3000CC, Pdatasheet pdfdatasheet pdf datasheet pdf datasheet pdf500 Tests
      STAR3000C
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      More Images
      References
      Reviews
      -
      • Intended Use
        Pack Size: 50 Tests, 500 TestsBio-Rad HISTAR Detection System reagents are designed specifically to replace complex immunohistochemical techniques with a simple kit. This kit provides linking and labeling reagents intended for use with species specific primary antibodies for visualizing cellular antigens in tissue specimens. Visualization is accomplished using an ultra-sensitive indirect labeling method that utilizes novel polymer labeling technology. Secondary antibodies are polymerized directly with Horseradish peroxidase (HRP) into compact polymers bearing a high ratio of enzyme to antibody. This biotin-free system offers enhanced sensitivity and minimal background staining.

        The HISTAR Detection System is designed for the staining of Human tissue sections. This kit is suitable for use with mouse and rabbit primary antibodies only.

        All labeling and blocking reagents are pre-diluted for immediate use, and chromogen is provided in concentrated format. This product is suitable for use with the wide range of Bio-Rad ready-to-use or concentrated primary antibody reagents against human targets. Detailed instructions for use can be found in the complete Bio-Rad PDF datasheet, that can be accessed by clicking on the "Instructions For Use" link. The procedure has been briefly summarized below:

        1) Prepare tissue sections appropriately according to the requirements of the primary antibody being used e.g. apply antigen retrieval if necessary.
        2) Incubate paraffin-embedded sections with peroxide blocking reagent for 15 minutes at room temperature. Rinse with Phosphate Buffered Saline (PBS).
        3) Apply serum block and incubate for 15 minutes.
        4) Apply primary antibody and incubate for 30 minutes.
        5) Apply reagent boost and incubate for 20 minutes. Rinse twice with PBS (5 minutes per wash).
        6) Apply HRP polymer for 30 minutes, then rinse twice with PBS (5 minutes per wash).
        7) Apply DAB substrate solution for 3-5 minutes, then rinse with distilled water.
        [Discard unused substrate solution]
        8) Counterstain and mount slides using an aqueous mounting medium such as BUF058A.

        Pack Size: 150 TestsBio-Rad HISTAR Detection System reagents are designed specifically to replace complex immunohistochemical techniques with a simple kit. This kit provides linking and labeling reagents intended for use with species specific primary antibodies for visualizing cellular antigens in tissue specimens. Visualization is accomplished using an ultra-sensitive indirect labeling method that utilizes novel polymer labeling technology. Secondary antibodies are polymerized directly with Horseradish peroxidase (HRP) into compact polymers bearing a high ratio of enzyme to antibody. This biotin-free system offers enhanced sensitivity and minimal background staining.

        The HISTAR Detection System is designed for the staining of Human tissue sections only. This kit is suitable for use with mouse and rabbit primary antibodies only.

        All labeling and blocking reagents are pre-diluted for immediate use, and chromogen is provided in concentrated format. This product is suitable for use with the wide range of Bio-Rad ready-to-use or concentrated primary antibody reagents against human targets. Detailed instructions for use can be found in the complete Bio-Rad PDF datasheet, that can be accessed by clicking on the "Instructions For Use" link. The procedure has been briefly summarized below:

        1) Prepare tissue sections appropriately according to the requirements of the primary antibody being used e.g. apply antigen retrieval if necessary.
        2) Incubate paraffin-embedded sections with peroxide blocking reagent for 15 minutes at room temperature. Rinse with Phosphate Buffered Saline (PBS).
        3) Apply serum block and incubate for 15 minutes.
        4) Apply primary antibody and incubate for 30 minutes.
        5) Apply reagent boost and incubate for 20 minutes. Rinse twice with PBS (5 minutes per wash).
        6) Apply HRP polymer for 30 minutes, then rinse twice with PBS (5 minutes per wash).
        7) Apply DAB substrate solution for 3-5 minutes, then rinse with distilled water.
        [Discard unused substrate solution]
        8) Counterstain and mount slides using an aqueous mounting medium such as BUF058A.

        Pack Size: 50 Tests, 500 TestsBio-Rad HISTAR Detection System reagents are designed specifically to replace complex immunohistochemical techniques with a simple kit. This kit provides linking and labeling reagents intended for use with species specific primary antibodies for visualizing cellular antigens in tissue specimens. Visualization is accomplished using an ultra-sensitive indirect labeling method that utilizes novel polymer labeling technology. Secondary antibodies are polymerized directly with Horseradish peroxidase (HRP) into compact polymers bearing a high ratio of enzyme to antibody. This biotin-free system offers enhanced sensitivity and minimal background staining.

        The HISTAR Detection System is designed for the staining of Human tissue sections. This kit is suitable for use with mouse and rabbit primary antibodies only.

        All labeling and blocking reagents are pre-diluted for immediate use, and chromogen is provided in concentrated format. This product is suitable for use with the wide range of Bio-Rad ready-to-use or concentrated primary antibody reagents against human targets. Detailed instructions for use can be found in the complete Bio-Rad PDF datasheet, that can be accessed by clicking on the "Instructions For Use" link. The procedure has been briefly summarized below:

        1) Prepare tissue sections appropriately according to the requirements of the primary antibody being used e.g. apply antigen retrieval if necessary.
        2) Incubate paraffin-embedded sections with peroxide blocking reagent for 15 minutes at room temperature. Rinse with Phosphate Buffered Saline (PBS).
        3) Apply serum block and incubate for 15 minutes.
        4) Apply primary antibody and incubate for 30 minutes.
        5) Apply reagent boost and incubate for 20 minutes. Rinse twice with PBS (5 minutes per wash).
        6) Apply HRP polymer for 30 minutes, then rinse twice with PBS (5 minutes per wash).
        7) Apply DAB substrate solution for 3-5 minutes, then rinse with distilled water.
        [Discard unused substrate solution]
        8) Counterstain and mount slides using an aqueous mounting medium such as BUF058A.

        Pack Size: 150 TestsBio-Rad HISTAR Detection System reagents are designed specifically to replace complex immunohistochemical techniques with a simple kit. This kit provides linking and labeling reagents intended for use with species specific primary antibodies for visualizing cellular antigens in tissue specimens. Visualization is accomplished using an ultra-sensitive indirect labeling method that utilizes novel polymer labeling technology. Secondary antibodies are polymerized directly with Horseradish peroxidase (HRP) into compact polymers bearing a high ratio of enzyme to antibody. This biotin-free system offers enhanced sensitivity and minimal background staining.

        The HISTAR Detection System is designed for the staining of Human tissue sections only. This kit is suitable for use with mouse and rabbit primary antibodies only.

        All labeling and blocking reagents are pre-diluted for immediate use, and chromogen is provided in concentrated format. This product is suitable for use with the wide range of Bio-Rad ready-to-use or concentrated primary antibody reagents against human targets. Detailed instructions for use can be found in the complete Bio-Rad PDF datasheet, that can be accessed by clicking on the "Instructions For Use" link. The procedure has been briefly summarized below:

        1) Prepare tissue sections appropriately according to the requirements of the primary antibody being used e.g. apply antigen retrieval if necessary.
        2) Incubate paraffin-embedded sections with peroxide blocking reagent for 15 minutes at room temperature. Rinse with Phosphate Buffered Saline (PBS).
        3) Apply serum block and incubate for 15 minutes.
        4) Apply primary antibody and incubate for 30 minutes.
        5) Apply reagent boost and incubate for 20 minutes. Rinse twice with PBS (5 minutes per wash).
        6) Apply HRP polymer for 30 minutes, then rinse twice with PBS (5 minutes per wash).
        7) Apply DAB substrate solution for 3-5 minutes, then rinse with distilled water.
        [Discard unused substrate solution]
        8) Counterstain and mount slides using an aqueous mounting medium such as BUF058A.

        Pack Size: 50 Tests, 500 TestsBio-Rad HISTAR Detection System reagents are designed specifically to replace complex immunohistochemical techniques with a simple kit. This kit provides linking and labeling reagents intended for use with species specific primary antibodies for visualizing cellular antigens in tissue specimens. Visualization is accomplished using an ultra-sensitive indirect labeling method that utilizes novel polymer labeling technology. Secondary antibodies are polymerized directly with Horseradish peroxidase (HRP) into compact polymers bearing a high ratio of enzyme to antibody. This biotin-free system offers enhanced sensitivity and minimal background staining.

        The HISTAR Detection System is designed for the staining of Human tissue sections. This kit is suitable for use with mouse and rabbit primary antibodies only.

        All labeling and blocking reagents are pre-diluted for immediate use, and chromogen is provided in concentrated format. This product is suitable for use with the wide range of Bio-Rad ready-to-use or concentrated primary antibody reagents against human targets. Detailed instructions for use can be found in the complete Bio-Rad PDF datasheet, that can be accessed by clicking on the "Instructions For Use" link. The procedure has been briefly summarized below:

        1) Prepare tissue sections appropriately according to the requirements of the primary antibody being used e.g. apply antigen retrieval if necessary.
        2) Incubate paraffin-embedded sections with peroxide blocking reagent for 15 minutes at room temperature. Rinse with Phosphate Buffered Saline (PBS).
        3) Apply serum block and incubate for 15 minutes.
        4) Apply primary antibody and incubate for 30 minutes.
        5) Apply reagent boost and incubate for 20 minutes. Rinse twice with PBS (5 minutes per wash).
        6) Apply HRP polymer for 30 minutes, then rinse twice with PBS (5 minutes per wash).
        7) Apply DAB substrate solution for 3-5 minutes, then rinse with distilled water.
        [Discard unused substrate solution]
        8) Counterstain and mount slides using an aqueous mounting medium such as BUF058A.

        Pack Size: 150 TestsBio-Rad HISTAR Detection System reagents are designed specifically to replace complex immunohistochemical techniques with a simple kit. This kit provides linking and labeling reagents intended for use with species specific primary antibodies for visualizing cellular antigens in tissue specimens. Visualization is accomplished using an ultra-sensitive indirect labeling method that utilizes novel polymer labeling technology. Secondary antibodies are polymerized directly with Horseradish peroxidase (HRP) into compact polymers bearing a high ratio of enzyme to antibody. This biotin-free system offers enhanced sensitivity and minimal background staining.

        The HISTAR Detection System is designed for the staining of Human tissue sections only. This kit is suitable for use with mouse and rabbit primary antibodies only.

        All labeling and blocking reagents are pre-diluted for immediate use, and chromogen is provided in concentrated format. This product is suitable for use with the wide range of Bio-Rad ready-to-use or concentrated primary antibody reagents against human targets. Detailed instructions for use can be found in the complete Bio-Rad PDF datasheet, that can be accessed by clicking on the "Instructions For Use" link. The procedure has been briefly summarized below:

        1) Prepare tissue sections appropriately according to the requirements of the primary antibody being used e.g. apply antigen retrieval if necessary.
        2) Incubate paraffin-embedded sections with peroxide blocking reagent for 15 minutes at room temperature. Rinse with Phosphate Buffered Saline (PBS).
        3) Apply serum block and incubate for 15 minutes.
        4) Apply primary antibody and incubate for 30 minutes.
        5) Apply reagent boost and incubate for 20 minutes. Rinse twice with PBS (5 minutes per wash).
        6) Apply HRP polymer for 30 minutes, then rinse twice with PBS (5 minutes per wash).
        7) Apply DAB substrate solution for 3-5 minutes, then rinse with distilled water.
        [Discard unused substrate solution]
        8) Counterstain and mount slides using an aqueous mounting medium such as BUF058A.

      • Product Form
      • Reconstitution
      • Preparation
      • Preservative Stabilisers
      • Purity
      • Approx. Protein Concentrations
      • Reagents In The Kit
        Pack Size: 50 TestsVIAL 1, Serum Block
        1 x 5ml, 5% Normal Serum in 0.01M Phosphate Buffered Saline (PBS) containing 0.1% Sodium Azide (NaN3)
        VIAL 2, Reagent Boost
        1 x 5ml
        VIAL 3, HRP Polymer
        1 x 5ml
        VIAL 4, Peroxide Blocking Reagent
        1 x 5ml, 0.03% Hydrogen Peroxide (H202)

        VIAL A, DAB Chromogen
        1 x 2ml, 2.5% 3,3’ Diaminobenzidine in stabilizing buffer
        VIAL B, DAB Substrate Buffer
        4 x 6ml, 0.1% H2O2 in Citric acid buffer

        Pack Size: 150 TestsVIAL 1, Serum Block
        1 x 15ml

        VIAL 2, Reagent Boost
        1 x 15ml

        VIAL 3, HRP Polymer
        1 x 15ml
        Pack Size: 500 TestsVIAL 1, Serum Block
        1 x 50ml

        VIAL 2, Reagent Boost
        1 x 50ml

        VIAL 3, HRP Polymer
        1 x 50ml
        Pack Size: 50 TestsVIAL 1, Serum Block
        1 x 5ml, 5% Normal Serum in 0.01M Phosphate Buffered Saline (PBS) containing 0.1% Sodium Azide (NaN3)
        VIAL 2, Reagent Boost
        1 x 5ml
        VIAL 3, HRP Polymer
        1 x 5ml
        VIAL 4, Peroxide Blocking Reagent
        1 x 5ml, 0.03% Hydrogen Peroxide (H202)

        VIAL A, DAB Chromogen
        1 x 2ml, 2.5% 3,3’ Diaminobenzidine in stabilizing buffer
        VIAL B, DAB Substrate Buffer
        4 x 6ml, 0.1% H2O2 in Citric acid buffer

        Pack Size: 150 TestsVIAL 1, Serum Block
        1 x 15ml

        VIAL 2, Reagent Boost
        1 x 15ml

        VIAL 3, HRP Polymer
        1 x 15ml
        Pack Size: 500 TestsVIAL 1, Serum Block
        1 x 50ml

        VIAL 2, Reagent Boost
        1 x 50ml

        VIAL 3, HRP Polymer
        1 x 50ml
        Pack Size: 50 TestsVIAL 1, Serum Block
        1 x 5ml, 5% Normal Serum in 0.01M Phosphate Buffered Saline (PBS) containing 0.1% Sodium Azide (NaN3)
        VIAL 2, Reagent Boost
        1 x 5ml
        VIAL 3, HRP Polymer
        1 x 5ml
        VIAL 4, Peroxide Blocking Reagent
        1 x 5ml, 0.03% Hydrogen Peroxide (H202)

        VIAL A, DAB Chromogen
        1 x 2ml, 2.5% 3,3’ Diaminobenzidine in stabilizing buffer
        VIAL B, DAB Substrate Buffer
        4 x 6ml, 0.1% H2O2 in Citric acid buffer

        Pack Size: 150 TestsVIAL 1, Serum Block
        1 x 15ml

        VIAL 2, Reagent Boost
        1 x 15ml

        VIAL 3, HRP Polymer
        1 x 15ml
        Pack Size: 500 TestsVIAL 1, Serum Block
        1 x 50ml

        VIAL 2, Reagent Boost
        1 x 50ml

        VIAL 3, HRP Polymer
        1 x 50ml
      • Preparing The Antibody
      • Test Principle
      • Buffer Solution
      • Storage
        Store at +4oC. DO NOT FREEZE.
        This product should be stored undiluted.
        Store at +4oC. DO NOT FREEZE.
        This product should be stored undiluted.
        Store at +4oC. DO NOT FREEZE.
        This product should be stored undiluted.
      • Shelf Life
        Please see label for expiry date.
        Please see label for expiry date.
        Please see label for expiry date.
      • Acknowledgements
      • Application NameYesNoMin DilutionMax Dilution
        Immunohistology - FrozenReady to use
        Immunohistology - ParaffinReady to use
      • Application NameYesNoMin DilutionMax Dilution
        Immunohistology - FrozenReady to use
        Immunohistology - ParaffinReady to use
      • Application NameYesNoMin DilutionMax Dilution
        Immunohistology - FrozenReady to use
        Immunohistology - ParaffinReady to use
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • ELISA
      • ELISA
      • ELISA
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Instructions For Use
        Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/STAR3000C.pdf
      • Instructions For Use
        Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/STAR3000A.pdf
      • Instructions For Use
        Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/STAR3000B.pdf

      Additional Histar Detection Kit Formats

      Formats Applications Sizes available
      Histar Detection Kit : Kit C, P 500 Tests | 150 Tests | 50 Tests

      Recommended Secondary Antibody

        Recommended Negative Isotype Control

          Useful Reagents

            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            10X Phosphate Buffered SalineBUF036A1 LitreE, IY
            BUF036A
            Permanent Aqueous Mounting MediumBUF058C250 mlC, P
            BUF058C
            Peroxide Blocking ReagentBUF017B50 mlP
            BUF017B
            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            10X Phosphate Buffered SalineBUF036A1 LitreE, IY
            BUF036A
            Permanent Aqueous Mounting MediumBUF058A30 mlC, P
            BUF058A
            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            10X Phosphate Buffered SalineBUF036A1 LitreE, IY
            BUF036A
            Permanent Aqueous Mounting MediumBUF058B100 mlC, P
            BUF058B

            Recommended Positive Controls

              Histology Controls

                References

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