Histar Detection Kit

//www.bio-rad-antibodies.com/histar-detection-kit-antibody-kit-star3000a.html
Histar Detection Kit gallery image 1

Detection of Mouse anti Troponin T carciac isoform 1 on formalin fixed, paraffin embedded human heart tissue using the Histar detection system (STAR3000A)

Enlarge
Histar Detection Kit gallery image 2

Detection of Mouse anti strep-tag-immo (MCA2488) in an immunohistochemical analysis of strep tagged Human anti Human CD276 (HCA143) on fomalin fixed, paraffin embedded prostate chippings followign heat mediated antigen retrieval with citrate buffer, pH6.0 using the Histar detection system (STAR3000A)

Enlarge
Histar Detection Kit gallery image 3

Detection of Mouse anti strep-tag-immo (MCA2488) in an immunohistochemical analysis of strep tagged Human anti Human CD127 (HCA147) on acetone fixed human tonsil using the Histar detection kit (STAR3000A)

Enlarge
Histar Detection Kit gallery image 4

Detection of Mouse anti strep-tag-immo (MCA2488) in an immunohistochemical analysis of strep tagged Human anti Human PINK1 (HCA150) on formalin fixed, paraffin embedded human testis following heat mediated antigen retrieval with citrate buffer, pH6.0 using the Histar detection kit (STAR3000A)

Enlarge
Histar Detection Kit gallery image 5

Detection of Mouse anti Human estrogen receptor (MCA1974) in an immunohistochemical analysis of estrogen receptor expression on formalin fixed, paraffin embedded human placenta following heat mediated antigen retrieval with citrate buffer, pH6.0 using the Histar detection kit (STAR300A)

Enlarge
Histar Detection Kit gallery image 6

Detection of Mouse anti Human estrogen receptor (MCA1974) in an immunohistochemical analysis of estrogen receptor expression on formalin fixed, paraffin embedded human breast cancer tissue folowing heat mediated antigen retrieval with EDTA, pH8.0 using the Histar detection kit (STAR3000A)

Enlarge
Histar Detection Kit gallery image 7

Detection of Mouse anti Human chromogranin A (MCA5696) in an immunohistochemical analysis of formalin fixed, paraffin embedded human pancreas following heat mediated antigen retrieval with citrate buffer, pH6.0 using the Histar detection kit (STAR3000A)

Enlarge
Histar Detection Kit gallery image 8

Detection of Mouse anti Human prostate specific antigen (MCA5842G) in an immunohistochemical analysis od PSA expression on formalin fixed, paraffin embedded human prostate using the Histar detection kit (STAR3000A), original magnification x100

Enlarge
Histar Detection Kit gallery image 9

Detection of Mouse anti Human prostate specific antigen (MCA5842G) in an immunohistochemical analysis od PSA expression on formalin fixed, paraffin embedded human prostate using the Histar detection kit (STAR3000A), original magnification x200

Enlarge
Histar Detection Kit gallery image 10

Detection of Mouse anti Human prostate specific antigen (MCA5843G) in an immunohistochemical analysis od PSA expression on formalin fixed, paraffin embedded human prostate using the Histar detection kit (STAR3000A), original magnification x100

Enlarge
Histar Detection Kit gallery image 11

Detection of Mouse anti Human prostate specific antigen (MCA5843G) in an immunohistochemical analysis od PSA expression on formalin fixed, paraffin embedded human prostate using the Histar detection kit (STAR3000A), original magnification x200

Enlarge
Histar Detection Kit gallery image 12

Immunoperoxidase staining of human spleen cryosection with Mouse anti Human CD11b antibody, clone ICRF44 (MCA551) followed by the Histar detection system (STAR3000). High power

Enlarge
  • Histar Detection Kit thumbnail image 1
  • Histar Detection Kit thumbnail image 2
  • Histar Detection Kit thumbnail image 3
  • Histar Detection Kit thumbnail image 4
  • Histar Detection Kit thumbnail image 5
  • Histar Detection Kit thumbnail image 6
  • Histar Detection Kit thumbnail image 7
  • Histar Detection Kit thumbnail image 8
  • Histar Detection Kit thumbnail image 9
  • Histar Detection Kit thumbnail image 10
  • Histar Detection Kit thumbnail image 11
  • Histar Detection Kit thumbnail image 12
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Kits
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    STAR3000BC, Pdatasheet pdfdatasheet pdf datasheet pdf150 Tests
    STAR3000B
    STAR3000AC, Pdatasheet pdfdatasheet pdf datasheet pdf datasheet pdf datasheet pdf datasheet pdf datasheet pdf50 Tests
    STAR3000A
    STAR3000CC, Pdatasheet pdfdatasheet pdf datasheet pdf500 Tests
    STAR3000C
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Intended Use
      Pack Size: 50 Tests, 500 TestsBio-Rad HISTAR Detection System reagents are designed specifically to replace complex immunohistochemical techniques with a simple kit. This kit provides linking and labeling reagents intended for use with species specific primary antibodies for visualizing cellular antigens in tissue specimens. Visualization is accomplished using an ultra-sensitive indirect labeling method that utilizes novel polymer labeling technology. Secondary antibodies are polymerized directly with Horseradish peroxidase (HRP) into compact polymers bearing a high ratio of enzyme to antibody. This biotin-free system offers enhanced sensitivity and minimal background staining.

      The HISTAR Detection System is designed for the staining of Human tissue sections. This kit is suitable for use with mouse and rabbit primary antibodies only.

      All labeling and blocking reagents are pre-diluted for immediate use, and chromogen is provided in concentrated format. This product is suitable for use with the wide range of Bio-Rad ready-to-use or concentrated primary antibody reagents against human targets. Detailed instructions for use can be found in the complete Bio-Rad PDF datasheet, that can be accessed by clicking on the "Instructions For Use" link. The procedure has been briefly summarized below:

      1) Prepare tissue sections appropriately according to the requirements of the primary antibody being used e.g. apply antigen retrieval if necessary.
      2) Incubate paraffin-embedded sections with peroxide blocking reagent for 15 minutes at room temperature. Rinse with Phosphate Buffered Saline (PBS).
      3) Apply serum block and incubate for 15 minutes.
      4) Apply primary antibody and incubate for 30 minutes.
      5) Apply reagent boost and incubate for 20 minutes. Rinse twice with PBS (5 minutes per wash).
      6) Apply HRP polymer for 30 minutes, then rinse twice with PBS (5 minutes per wash).
      7) Apply DAB substrate solution for 3-5 minutes, then rinse with distilled water.
      [Discard unused substrate solution]
      8) Counterstain and mount slides using an aqueous mounting medium such as BUF058A.

      Pack Size: 150 TestsBio-Rad HISTAR Detection System reagents are designed specifically to replace complex immunohistochemical techniques with a simple kit. This kit provides linking and labeling reagents intended for use with species specific primary antibodies for visualizing cellular antigens in tissue specimens. Visualization is accomplished using an ultra-sensitive indirect labeling method that utilizes novel polymer labeling technology. Secondary antibodies are polymerized directly with Horseradish peroxidase (HRP) into compact polymers bearing a high ratio of enzyme to antibody. This biotin-free system offers enhanced sensitivity and minimal background staining.

      The HISTAR Detection System is designed for the staining of Human tissue sections only. This kit is suitable for use with mouse and rabbit primary antibodies only.

      All labeling and blocking reagents are pre-diluted for immediate use, and chromogen is provided in concentrated format. This product is suitable for use with the wide range of Bio-Rad ready-to-use or concentrated primary antibody reagents against human targets. Detailed instructions for use can be found in the complete Bio-Rad PDF datasheet, that can be accessed by clicking on the "Instructions For Use" link. The procedure has been briefly summarized below:

      1) Prepare tissue sections appropriately according to the requirements of the primary antibody being used e.g. apply antigen retrieval if necessary.
      2) Incubate paraffin-embedded sections with peroxide blocking reagent for 15 minutes at room temperature. Rinse with Phosphate Buffered Saline (PBS).
      3) Apply serum block and incubate for 15 minutes.
      4) Apply primary antibody and incubate for 30 minutes.
      5) Apply reagent boost and incubate for 20 minutes. Rinse twice with PBS (5 minutes per wash).
      6) Apply HRP polymer for 30 minutes, then rinse twice with PBS (5 minutes per wash).
      7) Apply DAB substrate solution for 3-5 minutes, then rinse with distilled water.
      [Discard unused substrate solution]
      8) Counterstain and mount slides using an aqueous mounting medium such as BUF058A.

      Pack Size: 50 Tests, 500 TestsBio-Rad HISTAR Detection System reagents are designed specifically to replace complex immunohistochemical techniques with a simple kit. This kit provides linking and labeling reagents intended for use with species specific primary antibodies for visualizing cellular antigens in tissue specimens. Visualization is accomplished using an ultra-sensitive indirect labeling method that utilizes novel polymer labeling technology. Secondary antibodies are polymerized directly with Horseradish peroxidase (HRP) into compact polymers bearing a high ratio of enzyme to antibody. This biotin-free system offers enhanced sensitivity and minimal background staining.

      The HISTAR Detection System is designed for the staining of Human tissue sections. This kit is suitable for use with mouse and rabbit primary antibodies only.

      All labeling and blocking reagents are pre-diluted for immediate use, and chromogen is provided in concentrated format. This product is suitable for use with the wide range of Bio-Rad ready-to-use or concentrated primary antibody reagents against human targets. Detailed instructions for use can be found in the complete Bio-Rad PDF datasheet, that can be accessed by clicking on the "Instructions For Use" link. The procedure has been briefly summarized below:

      1) Prepare tissue sections appropriately according to the requirements of the primary antibody being used e.g. apply antigen retrieval if necessary.
      2) Incubate paraffin-embedded sections with peroxide blocking reagent for 15 minutes at room temperature. Rinse with Phosphate Buffered Saline (PBS).
      3) Apply serum block and incubate for 15 minutes.
      4) Apply primary antibody and incubate for 30 minutes.
      5) Apply reagent boost and incubate for 20 minutes. Rinse twice with PBS (5 minutes per wash).
      6) Apply HRP polymer for 30 minutes, then rinse twice with PBS (5 minutes per wash).
      7) Apply DAB substrate solution for 3-5 minutes, then rinse with distilled water.
      [Discard unused substrate solution]
      8) Counterstain and mount slides using an aqueous mounting medium such as BUF058A.

      Pack Size: 150 TestsBio-Rad HISTAR Detection System reagents are designed specifically to replace complex immunohistochemical techniques with a simple kit. This kit provides linking and labeling reagents intended for use with species specific primary antibodies for visualizing cellular antigens in tissue specimens. Visualization is accomplished using an ultra-sensitive indirect labeling method that utilizes novel polymer labeling technology. Secondary antibodies are polymerized directly with Horseradish peroxidase (HRP) into compact polymers bearing a high ratio of enzyme to antibody. This biotin-free system offers enhanced sensitivity and minimal background staining.

      The HISTAR Detection System is designed for the staining of Human tissue sections only. This kit is suitable for use with mouse and rabbit primary antibodies only.

      All labeling and blocking reagents are pre-diluted for immediate use, and chromogen is provided in concentrated format. This product is suitable for use with the wide range of Bio-Rad ready-to-use or concentrated primary antibody reagents against human targets. Detailed instructions for use can be found in the complete Bio-Rad PDF datasheet, that can be accessed by clicking on the "Instructions For Use" link. The procedure has been briefly summarized below:

      1) Prepare tissue sections appropriately according to the requirements of the primary antibody being used e.g. apply antigen retrieval if necessary.
      2) Incubate paraffin-embedded sections with peroxide blocking reagent for 15 minutes at room temperature. Rinse with Phosphate Buffered Saline (PBS).
      3) Apply serum block and incubate for 15 minutes.
      4) Apply primary antibody and incubate for 30 minutes.
      5) Apply reagent boost and incubate for 20 minutes. Rinse twice with PBS (5 minutes per wash).
      6) Apply HRP polymer for 30 minutes, then rinse twice with PBS (5 minutes per wash).
      7) Apply DAB substrate solution for 3-5 minutes, then rinse with distilled water.
      [Discard unused substrate solution]
      8) Counterstain and mount slides using an aqueous mounting medium such as BUF058A.

      Pack Size: 50 Tests, 500 TestsBio-Rad HISTAR Detection System reagents are designed specifically to replace complex immunohistochemical techniques with a simple kit. This kit provides linking and labeling reagents intended for use with species specific primary antibodies for visualizing cellular antigens in tissue specimens. Visualization is accomplished using an ultra-sensitive indirect labeling method that utilizes novel polymer labeling technology. Secondary antibodies are polymerized directly with Horseradish peroxidase (HRP) into compact polymers bearing a high ratio of enzyme to antibody. This biotin-free system offers enhanced sensitivity and minimal background staining.

      The HISTAR Detection System is designed for the staining of Human tissue sections. This kit is suitable for use with mouse and rabbit primary antibodies only.

      All labeling and blocking reagents are pre-diluted for immediate use, and chromogen is provided in concentrated format. This product is suitable for use with the wide range of Bio-Rad ready-to-use or concentrated primary antibody reagents against human targets. Detailed instructions for use can be found in the complete Bio-Rad PDF datasheet, that can be accessed by clicking on the "Instructions For Use" link. The procedure has been briefly summarized below:

      1) Prepare tissue sections appropriately according to the requirements of the primary antibody being used e.g. apply antigen retrieval if necessary.
      2) Incubate paraffin-embedded sections with peroxide blocking reagent for 15 minutes at room temperature. Rinse with Phosphate Buffered Saline (PBS).
      3) Apply serum block and incubate for 15 minutes.
      4) Apply primary antibody and incubate for 30 minutes.
      5) Apply reagent boost and incubate for 20 minutes. Rinse twice with PBS (5 minutes per wash).
      6) Apply HRP polymer for 30 minutes, then rinse twice with PBS (5 minutes per wash).
      7) Apply DAB substrate solution for 3-5 minutes, then rinse with distilled water.
      [Discard unused substrate solution]
      8) Counterstain and mount slides using an aqueous mounting medium such as BUF058A.

      Pack Size: 150 TestsBio-Rad HISTAR Detection System reagents are designed specifically to replace complex immunohistochemical techniques with a simple kit. This kit provides linking and labeling reagents intended for use with species specific primary antibodies for visualizing cellular antigens in tissue specimens. Visualization is accomplished using an ultra-sensitive indirect labeling method that utilizes novel polymer labeling technology. Secondary antibodies are polymerized directly with Horseradish peroxidase (HRP) into compact polymers bearing a high ratio of enzyme to antibody. This biotin-free system offers enhanced sensitivity and minimal background staining.

      The HISTAR Detection System is designed for the staining of Human tissue sections only. This kit is suitable for use with mouse and rabbit primary antibodies only.

      All labeling and blocking reagents are pre-diluted for immediate use, and chromogen is provided in concentrated format. This product is suitable for use with the wide range of Bio-Rad ready-to-use or concentrated primary antibody reagents against human targets. Detailed instructions for use can be found in the complete Bio-Rad PDF datasheet, that can be accessed by clicking on the "Instructions For Use" link. The procedure has been briefly summarized below:

      1) Prepare tissue sections appropriately according to the requirements of the primary antibody being used e.g. apply antigen retrieval if necessary.
      2) Incubate paraffin-embedded sections with peroxide blocking reagent for 15 minutes at room temperature. Rinse with Phosphate Buffered Saline (PBS).
      3) Apply serum block and incubate for 15 minutes.
      4) Apply primary antibody and incubate for 30 minutes.
      5) Apply reagent boost and incubate for 20 minutes. Rinse twice with PBS (5 minutes per wash).
      6) Apply HRP polymer for 30 minutes, then rinse twice with PBS (5 minutes per wash).
      7) Apply DAB substrate solution for 3-5 minutes, then rinse with distilled water.
      [Discard unused substrate solution]
      8) Counterstain and mount slides using an aqueous mounting medium such as BUF058A.

    • Product Form
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
      Pack Size: 50 TestsVIAL 1, Serum Block
      1 x 5ml, 5% Normal Serum in 0.01M Phosphate Buffered Saline (PBS) containing 0.1% Sodium Azide (NaN3)
      VIAL 2, Reagent Boost
      1 x 5ml
      VIAL 3, HRP Polymer
      1 x 5ml
      VIAL 4, Peroxide Blocking Reagent
      1 x 5ml, 0.03% Hydrogen Peroxide (H202)

      VIAL A, DAB Chromogen
      1 x 2ml, 2.5% 3,3’ Diaminobenzidine in stabilizing buffer
      VIAL B, DAB Substrate Buffer
      4 x 6ml, 0.1% H2O2 in Citric acid buffer

      Pack Size: 150 TestsVIAL 1, Serum Block
      1 x 15ml

      VIAL 2, Reagent Boost
      1 x 15ml

      VIAL 3, HRP Polymer
      1 x 15ml
      Pack Size: 500 TestsVIAL 1, Serum Block
      1 x 50ml

      VIAL 2, Reagent Boost
      1 x 50ml

      VIAL 3, HRP Polymer
      1 x 50ml
      Pack Size: 50 TestsVIAL 1, Serum Block
      1 x 5ml, 5% Normal Serum in 0.01M Phosphate Buffered Saline (PBS) containing 0.1% Sodium Azide (NaN3)
      VIAL 2, Reagent Boost
      1 x 5ml
      VIAL 3, HRP Polymer
      1 x 5ml
      VIAL 4, Peroxide Blocking Reagent
      1 x 5ml, 0.03% Hydrogen Peroxide (H202)

      VIAL A, DAB Chromogen
      1 x 2ml, 2.5% 3,3’ Diaminobenzidine in stabilizing buffer
      VIAL B, DAB Substrate Buffer
      4 x 6ml, 0.1% H2O2 in Citric acid buffer

      Pack Size: 150 TestsVIAL 1, Serum Block
      1 x 15ml

      VIAL 2, Reagent Boost
      1 x 15ml

      VIAL 3, HRP Polymer
      1 x 15ml
      Pack Size: 500 TestsVIAL 1, Serum Block
      1 x 50ml

      VIAL 2, Reagent Boost
      1 x 50ml

      VIAL 3, HRP Polymer
      1 x 50ml
      Pack Size: 50 TestsVIAL 1, Serum Block
      1 x 5ml, 5% Normal Serum in 0.01M Phosphate Buffered Saline (PBS) containing 0.1% Sodium Azide (NaN3)
      VIAL 2, Reagent Boost
      1 x 5ml
      VIAL 3, HRP Polymer
      1 x 5ml
      VIAL 4, Peroxide Blocking Reagent
      1 x 5ml, 0.03% Hydrogen Peroxide (H202)

      VIAL A, DAB Chromogen
      1 x 2ml, 2.5% 3,3’ Diaminobenzidine in stabilizing buffer
      VIAL B, DAB Substrate Buffer
      4 x 6ml, 0.1% H2O2 in Citric acid buffer

      Pack Size: 150 TestsVIAL 1, Serum Block
      1 x 15ml

      VIAL 2, Reagent Boost
      1 x 15ml

      VIAL 3, HRP Polymer
      1 x 15ml
      Pack Size: 500 TestsVIAL 1, Serum Block
      1 x 50ml

      VIAL 2, Reagent Boost
      1 x 50ml

      VIAL 3, HRP Polymer
      1 x 50ml
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
    • Additional Reagents Required
      1) Primary antibody and positive control
      2) Phosphate Buffered Saline (PBS), pH7.4 (+ 0.2)
      3) Counterstain
      4) Mounting media

      Bio-Rad can supply a number of these products separately, please see the ‘Recommended Useful Reagents’ section.
    • Storage
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted.
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted.
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted.
    • Shelf Life
      6 months from the date of despatch.
      6 months from the date of despatch.
      Please see label for expiry date.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - FrozenReady to use
      Immunohistology - ParaffinReady to use
    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - FrozenReady to use
      Immunohistology - ParaffinReady to use
    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - FrozenReady to use
      Immunohistology - ParaffinReady to use

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
      Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/STAR3000C.pdf
    • Instructions For Use
      Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/STAR3000A.pdf
    • Instructions For Use
      Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/STAR3000B.pdf

    Additional Histar Detection Kit Formats

    Formats Applications Sizes available
    Histar Detection Kit : Kit C, P 500 Tests | 150 Tests | 50 Tests
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          10X Phosphate Buffered SalineBUF036A1 LitreIY
          BUF036A
          Permanent Aqueous Mounting MediumBUF058C250 mlC, P
          BUF058C
          Peroxide Blocking ReagentBUF017B50 mlP
          BUF017B
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          10X Phosphate Buffered SalineBUF036A1 LitreIY
          BUF036A
          Permanent Aqueous Mounting MediumBUF058A30 mlC, P
          BUF058A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          10X Phosphate Buffered SalineBUF036A1 LitreIY
          BUF036A
          Permanent Aqueous Mounting MediumBUF058B100 mlC, P
          BUF058B

          Recommended Positive Controls

            Histology Controls

              References

              Write your review

              You may also be interested in...

              You May Also Be Interested In...