CD4 Antibody | CT7

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CD4 Antibody | CT7 gallery image 1

Peripheral Guinea Pig Lymphocytes stained with Mouse anti Guinea Pig CD4:FITC (MCA749F)

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CD4 Antibody | CT7 gallery image 2

Staining of Guinea Pig spleen cells with Mouse anti Guinea Pig CD4:RPE (MCA749PE)

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CD4 Antibody | CT7 gallery image 3

Published customer image:
Mouse anti Guinea Pig CD4 antibody, clone CT7 used for the detection of CD4 expressing cells in the guinea pig airway by immunohistochemistry on formalin fixed, paraffin embedded sections.
Image caption:
Immunohistochemistry staining for CD4+ cells was not increased in animals neonatally sensitized compared with control animals. Representative slides of airway sections from adult animals immunostained for CD4+ cells are shown in the top and middle panels. The arrow shows an area of the epithelium with intense positive staining for CD4. Computerized quantification of CD4+ staining, expressed as % area of the airway wall, in control guinea pigs and animals sensitized at birth is shown in the bottom panel as means and standard errors, n = 5, n = 5, and n = 5 for 1-week, 3-week, and adult control animals, n = 5, n = 6, and n = 5 for 1-week, 3-week, and adult sensitized animals, respectively. A significant reduction was observed in the tissue of the 3-week-sensitized group compared to 3-week controls (P < 0.05).

From: Chitano P, Wang L, Degan S, Worthington CL, Pozzato V, Hussaini SH, Turner WC, Dorscheid DR, Murphy TM. Ovalbumin sensitization of guinea pig at birth prevents the ontogenetic decrease in airway smooth muscle responsiveness.
Physiol Rep. 2014 Dec 11;2(12). pii: e12241.

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CD4 Antibody | CT7 gallery image 4

Published customer image:
Mouse anti Guinea Pig CD4 antibody, clone CT7 used for the detection of CD4 expressing cells in the guinea pig lungs by flow cytometry
Image caption:
Monitoring of activated T cells in the infected lungs during therapy. Percentage of activated CD4+ T cells (A) and CD8+ T cells (B) in drug or drug plus MIP group were evaluated at different time points during the course of therapy. The percentage of respective cells in infected control group has been shown in the inset of bar diagram. Activated T cells were analyzed based on the expression of homing receptors as shown in the representative contour plots and compared at 15 days and 30 days of therapy. In each plot, region R6 represents T cells with high homing receptor expression and the corresponding percentage value is mentioned inside the region. Data represents the mean value of four animals in each group. * p<0.05. Uninfected animal (naive) was taken as uninfected negative control and untreated animal at 30 days post infection was taken as infected positive control.

From: Gupta A, Ahmad FJ, Ahmad F, Gupta UD, Natarajan M, et al. (2012) Efficacy of Mycobacterium indicus pranii Immunotherapy as an Adjunct to Chemotherapy for Tuberculosis and Underlying Immune Responses in the Lung.
PLoS ONE 7(7): e39215.

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CD4 Antibody | CT7 gallery image 5

Published customer image:
Mouse anti Guinea Pig CD4 antibody, clone CT7 used for the detection of CD4 expressing cells in the guinea pig lungs by immunohistochemistry on formailin fixed cryosections.
Image caption:
Evaluation of cellular immune response inside the granuloma of infected lungs. Scattergrams show the numbers of CD4+ T cells, CD8+ T cells, granulocytes and MHC II+ cells per unit area of granuloma from different experimental groups after 30 days of therapy. The number of different immune cells inside the granuloma in the lung sections from different experimental groups were counted microscopically and expressed as number of cells per unit area of granuloma. Minimum of 1000 cells were counted from randomly selected, at least 10 fields. Sections were taken from lung samples of 4 animals per group. The representative photomicrographs show the positively stained cells with red arrow in control group, drug or drug + MIP group in each panel. DAB was used as substrate in staining granulocytes while VIP red was substrate for staining other types of cells. * p<0.05, ** p<0.01.

From: Gupta A, Ahmad FJ, Ahmad F, Gupta UD, Natarajan M, et al. (2012) Efficacy of Mycobacterium indicus pranii Immunotherapy as an Adjunct to Chemotherapy for Tuberculosis and Underlying Immune Responses in the Lung.
PLoS ONE 7(7): e39215.

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  • Product Type
    Monoclonal Antibody
  • Clone
    CT7
  • Isotype
    IgG1
3 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA749SC, Fdatasheet pdfdatasheet pdf0.25 ml
    MCA749S
    MCA749PEFdatasheet pdfdatasheet pdf100 Tests
    MCA749PE
    MCA749FFdatasheet pdfdatasheet pdf100 Tests
    MCA749F
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Mouse anti Guinea Pig CD4 antibody, clone CT7 recognizes the CD4 antigen present on T Helper/Inducer lymphocytes.
    • Intended Use
    • Target Species
      Guinea Pig
    • Product Form
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Concentrated tissue Culture Supernatant - liquid
    • Reconstitution
      Reconstitute with 1.0ml distilled water
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G.
      Purified IgG prepared by affinity chromatography on Protein G.
    • Preservative Stabilisers
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.1% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
    • Immunogen
      Guinea pig peritoneal T-cells.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.1 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised BALB/c mice were fused with cells of the X63.Ag8.653 mouse myeloma cell line.
    • Storage
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC for one month or at -20oC for longer.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC for one month or at -20oC for longer.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution.
      18 months from the date of despatch.
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/101/100
      Immunohistology - Frozen1/101/100

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells or 100ul whole Guinea Pig peripheral blood.
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD4 Antibody Formats

    Formats Clone Applications Sizes available
    CD4 Antibody : FITC CT7 F 100 Tests
    CD4 Antibody : Con S/N CT7 C, F 0.25 ml
    CD4 Antibody : RPE CT7 F 100 Tests
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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