Bu-1a/b Antibody | BoA1

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Bu-1a/b Antibody | BoA1 gallery image 1

Mouse anti Guinea Fowl Bu-1a/b, clone BoA1, immunohistochemical staining of frozen sections from 4 week old chicken bursa of fabricus. Image courtesy of Dr Nandor Nagy, Semmelweis University, Hungary

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Mouse anti Guinea Fowl Bu-1a/b, clone BoA1, staining of acetone fixed cryostat sections from 4 week old chicken spleen. Clone BoA1 recognizes Bu1+ (chB6+) B cells of the germinal center (GC) and peri-ellipsoidal white pulp (arrow). Image courtesy of Dr Nandor Nagy, Semmelweis University, Hungary

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Mouse anti Guinea Fowl Bu-1a/b, clone BoA1 immunohistological staining of cryostat sections from 4 week old Grey Partridge (Perdix perdix) bursa of Fabricius. Clone BoA1 recognizes B cells of the follicles. Image courtesy of Dr Nandor Nagy, Semmelweis University, Hungary

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Mouse anti Guinea Fowl Bu-1a/b, clone BoA1 immunohistological staining of paraformaldehide fixed frozen sections of caecal tonsil from 6 week old chicken tissues. Clone BoA1 recognizes B cells occuring in the lamina propria germinal centers (GC). Image courtesy of Dr Nandor Nagy, Semmelweis University, Hungary

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Bu-1a/b Antibody | BoA1 gallery image 5

Mouse anti Guinea Fowl Bu-1a/b, clone BoA1, immunohistological staining of cryostat sections from 6 week old guinea fowl (Numida meleagris) bursa of Fabricius. Clone BoA1 recognizes B cells of the follicles. Image courtesy of Dr Nandor Nagy, Semmelweis University, Hungary

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  • Product Type
    Monoclonal Antibody
  • Clone
    BoA1
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA5958GAC, IP, WBdatasheet pdfdatasheet pdf0.1 mg
    MCA5958GA
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Mouse anti Guinea Fowl Bu-1a/b antibody, clone BoA1 specifically recognizes the avian bursal cell antigen, Bu-1a/b. Bu-1a/b, also known as chB6, is a type 1 transmembrane protein.

      Bu-1 is a product of two alleles, Bu-1a (chB6.1) and Bu-1b (chB6.2).Mouse anti Guinea Fowl Bu-1a/b antibody, clone BoA1 recognizes both of these alleles. The Bu-1 antigen is expressed by chicken B-cells throughout most of their development and by a subset of monocytes and macrophages (Igyártó et al. 2008) but is absent from erythrocytes, granulocytes and thrombocytes. As such the Mouse anti Guinea Fowl Bu-1a/b antibody, clone BoA1 provides a useful marker of avian B-cells.

      Clone BoA1 is unique in that while other antibodies generated against the Bu-1a/b antigen are restricted in their species reactivity, clone BoA1 recognises the Bu-1a/b antigen in all phasianid species tested to date. Clone BoA1 therefore provides a reagent that facilitates comparative studies in both domestic and wild phasianids.

      In addition to Mouse anti Guinea Fowl Bu-1a/b antibody, clone BoA1, clone AV20 which is specific to chicken Bu1-a/b and clone L22 which recognizes the Bu-1a but not the Bu-1b allele in chicken and quail, are also available from Bio-Rad.
    • Intended Use
    • Target Species
      Guinea Fowl
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Quailyes
      Turkeyyes
      Chickenyes
      Grey partridgeyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
    • Preservative Stabilisers
      0.1% Sodium Azide (NaN3)
      0.1% Bovine Serum Albumin
    • Immunogen
      Bursal cell suspension from guinea fowl (Numida melagris).
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised Balb/c mice were fused with cells of the Sp2/0-Ag14 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen
      Immunohistology - Paraffin
      Immunoprecipitation
      Western Blotting

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Bu-1a/b Antibody Formats

    Formats Clone Applications Sizes available
    Bu-1a/b Antibody : Purified BoA1 C, IP, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Immunoprecipitation

              References

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