Human anti GST antibody, clone AbD02031 detects glutathione-S-transferase (GST), a family of cytosolic dimeric isoenzymes that play an important role in detoxification by catalyzing the conjugation of reduced glutathione to a myriad of hydrophobic and electrophilic compounds. GSTs may also bind toxins and function as transport proteins.
Mammalian GSTs are 45–55 kDa in size and are assigned to at least four generic classes: Alpha, Mu , Pi and Theta. The amino acid sequence GST is highly conserved in most organisms, including mammals.
GST is a commonly used tag in protein expression systems
A lyophilized, bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library. Expressed in E. coli and purified using metal chelate affinity chromatography. This Fab fragment is bivalent by dimerization of the bacterial alkaline phosphatase fusion protein. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain.
Reconstitute with 1.0 ml distilled water
Metal chelate affinity chromatography
0.09% Sodium Azide (NaN3)
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml after reconstitution
Reagents In The Kit
Preparing The Antibody
Phosphate buffered saline
Prior to reconstitution store at +4oC. After reconstitution store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended.
12 months from date of reconstitution
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany. His-tag is a registered trademark of EMD Biosciences.
For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.