Human anti golimumab (drug/target complex) specificity titration ELISAEnlarge
A microtiter plate was coated over night with human TNFα, golimumab or human IgG1/kappa at a concentration of 5 μg/ml. After washing and blocking with PBST+5% BSA, the human TNFα/golimumab complex was formed by adding 2 μg/ml golimumab to the wells coated with human TNFα. Detection was performed using Human anti golimumab (clone AbD25705, HCA274) titrated to the given concentrations in PBST, followed by Mouse anti His tag:HRP (MCA5995P) in HISPEC assay diluent (BUF049A) and QuantaBlu™ fluorogenic peroxidase substrate. Data for binding to human TNFα/golimumab are presented as the mean of three measurements.