Golimumab Antibody | AbD25418_hIgG1

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Human Anti-Golimumab Antibody specificity ELISA

A microtiter plate was coated overnight with various antigens at a concentration of 5 μg/ml. After washing and blocking with PBST + 5% BSA, detection was performed using Human Anti-Golimumab:HRP Antibody, clone AbD25418_hIgG1 (HCA287P) at a concentration of 2 μg/ml in HISPEC Assay Diluent (BUF049A) and QuantaBlu Fluorogenic Peroxidase Substrate.

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Human Anti-Golimumab Antibody specificity titration ELISA

A microtiter plate was coated overnight with golimumab, human TNFα, human IgG1/kappa, human IgG1/lambda or adalimumab at a concentration of 5 μg/ml. After washing and blocking with PBST + 5% BSA, detection was performed using Human Anti-Golimumab:HRP Antibody, clone AbD25418_hIgG1 (HCA287P) titrated to the given concentrations in HISPEC Assay Diluent (BUF049A) and QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements.

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Human Anti-Golimumab Antibody bridging ELISA for pharmacokinetic (PK) assay development

A microtiter plate was coated overnight with Human Anti-Golimumab Antibody, clone AbD25429 (HCA286) at a concentration of 1 μg/ml. After washing and blocking with PBST + 5% BSA, 10% human serum was added spiked with increasing concentrations of golimumab. Detection was performed using Human Anti-Golimumab:HRP Antibody, clone AbD25418_hIgG1 (HCA287P) at a concentration of 2 μg/ml in HISPEC Assay Diluent (BUF049A) and QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements.

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Golimumab bridging ELISA for anti-drug antibody (ADA) assay development

A microtiter plate was coated overnight with golimumab at a concentration of 1 μg/ml. After washing and blocking with PBST + 5% BSA, PBST with 10% human serum was added spiked with increasing concentrations of Human Anti-Golimumab Antibody, clone AbD25418_hIgG1. Detection was performed using HRP conjugated golimumab at a concentration of 2 μg/ml in HISPEC Assay Diluent (BUF049A) and QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements.

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Human Anti-Golimumab Antibody inhibition ELISA

A microtiter plate was coated overnight with Human Anti-Golimumab Antibody, clone AbD25418 in monovalent Fab format. After washing and blocking with PBST + 5% BSA, golimumab was added at the given concentrations, mixed either with 5-fold molar excess of TNFα (black line) or without TNFα (red line). Golimumab, still capable of binding to the Human Anti-Golimumab Antibody coated plate, was detected using Mouse Anti-Human IgG (Fc) CH2 Domain:HRP Antibody (MCA647P) and QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of two measurements.

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  • Human anti Golimumab:HRP
  • Human anti Golimumab
(Rated 0.0 out of 5 based on 0 customer reviews)
    Anti-Golimumab Antibody is a recombinant, inhibitory, anti-idiotypic antibody in IgG1 format, ideal for bioanalysis and immunogenicity testing of golimumab and biosimilars. It is suitable as a detection antibody in a PK bridging ELISA to detect free drug, and as a reference standard in an ADA assay.
    • Product Type
      Monoclonal Antibody
    • Clone
      AbD25418_hIgG1
    • Isotype
      IgG1
    2 Formats Available
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      HCA287PEdatasheet pdfdatasheet pdf0.1 mg
      HCA287P
      HCA287Edatasheet pdfdatasheet pdf0.1 mg
      HCA287
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      More Images
      References
      Reviews
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      • Human Anti-Golimumab Antibody, clone AbD25418_hIgG1 is a paratope specific, inhibitory anti-idiotypic antibody that specifically recognizes the free human monoclonal antibody golimumab and inhibits binding to its target TNFα.

        Clone AbD25418_hIgG1 does not recognize free human TNFα or golimumab in complex with human TNFα. This antibody can be used to measure free golimumab and biosimilar products in bioanalytical assays.

        Clone AbD25418_hIgG1 is an affinity-matured variant of AbD20692 (HCA241), with about a 21-fold affinity improvement.

        A pair of anti golimumab antibodies can be used to develop a pharmacokinetic (PK) bridging assay to measure free drug. This antibody, in full immunoglobulin format, is recommended as the detection antibody paired with clone AbD25429 (HCA286) in monovalent Fab format as the capture antibody.

        Golimumab, marketed under the brand name Simponi is a recombinant human IgG1/kappa monoclonal antibody approved for the treatment of rheumatoid arthritis, psoriatic arthritis and ankylosing spondylitis. This therapeutic antibody, directed against TNFα, acts by blocking the binding of TNFα to its receptors, resulting in a down-regulation of the inflammatory response associated with autoimmune diseases.

        View a summary of all anti-golimumab antibodies
      • Intended Use
      • Product Form
        Human IgG1 antibody (kappa light chain) selected from the HuCAL® phage display library and expressed in a human cell line. Conjugated to horseradish peroxidase (HRP) – liquid
        Human IgG1 antibody (kappa light chain) selected from the HuCAL® phage display library and expressed in a human cell line. This antibody is supplied as a liquid
      • Reconstitution
      • Preparation
        Purified IgG prepared by affinity chromatography on Protein A
        Purified IgG prepared by affinity chromatography on Protein A
      • Preservative Stabilisers
        0.01% Thiomersal
        0.01% Thiomersal
      • Immunogen
        Golimumab
      • Purity
      • Affinity
        The monovalent intrinsic affinity of AbD25418 was measured as KD = 0.13 nM by real time, label free molecular interaction analysis on immobilized golimumab
      • Approx. Protein Concentrations
        IgG concentration 0.1 mg/ml
        IgG concentration 0.5 mg/ml
      • Reagents In The Kit
      • Preparing The Antibody
      • Test Principle
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
      • Storage
        Store at -70oC.
        Storage in frost-free freezers is not recommended.
        This product should be stored undiluted.
        Store at +4oC or at -20oC if preferred.
        Storage in frost-free freezers is not recommended.
        This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use
      • Shelf Life
        12 months from date of despatch
        12 months from date of despatch
      • Acknowledgements
        Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany. Simponi is a registered trademark of Janssen Biotech, Inc. in the USA and Merck & Co, Inc. in Europe
        Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany. Simponi is a registered trademark of Janssen Biotech, Inc. in the USA and Merck & Co, Inc. in Europe
      • Licensed Use
        For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad
      • Regulatory
        For research purposes only
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

      • Application NameYesNoMin DilutionMax Dilution
        ELISA
      • Application NameYesNoMin DilutionMax Dilution
        ELISA

      • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
      • Technical Advice
        Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
      • Technical Advice
        Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
      • Recommended Protocol
      • Recommended Protocol
      • ELISA
        This product may be used in a direct ELISA or as detection antibody for golimumab in a bridging ELISA together with HCA286 (AbD25429, Fab-FH) as the capture reagent.
        Protocol: PK bridging ELISA to measure free drug
      • ELISA
        This product may be used in a direct or indirect ELISA or as detection antibody for golimumab in a bridging ELISA together with HCA286 (AbD25429) as the capture reagent.
        Protocol: PK bridging ELISA to measure free drug
      • Immunohistology
      • Immunohistology
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Immunofluorescence
      • Immunofluorescence
      • Western Blotting
      • Western Blotting
      • Instructions For Use
      • Instructions For Use

      Additional Golimumab Antibody Formats

      Formats Clone Applications Sizes available
      Golimumab Antibody : HRP AbD25418_hIgG1 E 0.1 mg
      Golimumab Antibody : Purified AbD25418_hIgG1 E 0.1 mg
      • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        Recommended Negative Isotype Control

          Useful Reagents

            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Human anti GolimumabHCA2860.1 mgE
            HCA286
            Hispec Assay DiluentBUF049A50 mlIY
            BUF049A
            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Human anti Golimumab:HRPHCA287P0.1 mgE
            HCA287P
            Mouse anti Human IgG (Fc) CH2 Domain:HRPMCA647P0.2 mgC *, E
            MCA647P
            LYNX Rapid HRP Antibody Conjugation KitLNK001P1 Conjugation For 400µg AntibodyCJ
            LNK001P
            Human anti GolimumabHCA2860.1 mgE
            HCA286
            Hispec Assay DiluentBUF049A50 mlIY
            BUF049A

            Recommended Positive Controls

              Histology Controls

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