Golimumab Antibody | AbD20893

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Human anti golimumab (drug/target complex) specificity ELISA

A microtiter plate was coated overnight with various antigens at a concentration of 5 µg/ml. After washing and blocking with PBST+5% BSA, detection was performed using Human anti golimumab (clone AbD20893) at a concentration of 2 µg/ml, followed by an HRP-labelled anti His-tag in HISPEC assay diluent (BUF049A) and QuantaBlu fluorogenic peroxidase substrate

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Human anti golimumab (drug/target complex) specificity titration ELISA

A microtiter plate was coated over night with human TNFα, golimumab or human IgG1/kappa at a concentration of 5 µg/ml. After washing and blocking with PBST+5% BSA, the TNFα/golimumab complex was formed by adding 2 µg/ml golimumab to TNFα-coated wells. Detection was performed using Human anti golimumab (clone AbD20893) titrated to the given concentrations in PBST, followed by an HRP-conjugated anti His-tag in HISPEC assay diluent (BUF049A) and QuantaBlu fluorogenic peroxidase substrate. Data for the binding of antibody to TNFα/golimumab is presented as the mean of three measurements

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Golimumab capture ELISA for Pharmacokinetic (PK) assay development

A microtiter plate was coated over night with human TNFα at a concentration of 5 µg/ml. After washing and blocking with PBST+5% BSA, 10% human serum was added spiked with increasing concentrations of golimumab. Detection was performed using human anti golimumab (clone AbD20893) at a concentration of 2 µg/ml, followed by an HRP-conjugated anti-His tag in HISPEC diluent (BUF049A) and QuantaBlu fluorogenic peroxidase substrate. Data for the binding of antibody to TNFα/golimumab is presented as the mean of three measurements

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  • Human anti Golimumab (Drug/Target Complex)
(Rated 0.0 out of 5 based on 0 customer reviews)
    Anti-golimumab drug-target complex antibody specifically recognizes the golimumab-TNF alpha complex and detects golimumab or biosimilars only when bound to TNF alpha. This antibody is in monovalent Fab format and is suitable for a PK antigen capture format assay to detect bound drug.
    • Product Type
      Monoclonal Antibody
    • Clone
      AbD20893
    • Isotype
      HuCAL Fab monovalent
    1 Formats Available
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      HCA244Edatasheet pdfdatasheet pdf0.1 mg
      HCA244
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      More Images
      References
      Reviews
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      • Human anti golimumab (drug/target complex), clone AbD20893 is a Type 3 anti-idiotypic antibody that specifically recognizes golimumab when bound to tumor necrosis factor alpha (TNFα) i.e. the drug/target complex. Clone AbD20893 does not recognize unbound golimumab nor free TNFα. Clone AbD20893 may be used to measure golimumab when captured by immobilized human TNFα or golimumab/TNFα levels in serum from patients.

        Golimumab, marketed under the brand name Simponi®, is a recombinant human IgG1/kappa monoclonal antibody approved for the treatment of rheumatoid arthritis, psoriatic arthritis and ankylosing spondylitis. This therapeutic antibody, directed against TNFα, acts by blocking the binding of TNFα to its receptors, resulting in a down-regulation of the inflammatory response associated with autoimmune diseases.

        Clone AbD20893 is one of a range of Type 1 (HCA240, HCA241), Type 2 (HCA242, HCA243) and Type 3 (HCA245) anti-idiotypic antibodies to golimumab available from Bio-Rad and forms part of a wider and expanding portfolio of anti-idiotypic antibodies that have been developed for the detection of biotherapeutics.
      • Intended Use
      • Product Form
        A monovalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
      • Reconstitution
      • Preparation
        Metal chelate affinity chromatography
      • Preservative Stabilisers
        0.01% Thiomersal
      • Immunogen
        Golimumab.
      • Purity
      • Affinity
        The monovalent intrinsic affinity of AbD20893 was measured as KD = 53 nM by real time, label free molecular interaction analysis on immobilized golimumab in complex with human TNFα.
      • Approx. Protein Concentrations
        Antibody concentration 0.5 mg/ml
      • Reagents In The Kit
      • Preparing The Antibody
      • Test Principle
      • Buffer Solution
        Phosphate buffered saline
      • Storage
        Store at +4oC or at -20oC if preferred.
        Storage in frost-free freezers is not recommended.
        This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      • Shelf Life
        12 months from date of despatch
      • Acknowledgements
        Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European Patent 0,859,841 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany, a wholly owned subsidiary of Bio-Rad Laboratories Inc.

        Simponi® is a registered trademark of Janssen Biotech, Inc. in the USA and Merck & Co, Inc. in Europe.
      • Licensed Use
        For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
      • Regulatory
        For research purposes only
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

      • Application NameYesNoMin DilutionMax Dilution
        ELISA

      • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
      • Technical Advice
        Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
      • Recommended Protocol
      • ELISA
        This product may be used in an indirect ELISA to detect the golimumab/TNFα complex or to detect golimumab bound to immobilized TNFα.
        Protocol: PK antigen capture ELISA to measure bound drug exclusively
      • Immunohistology
      • Histology Positive Control Tissue
      • Immunofluorescence
      • Western Blotting
      • Instructions For Use

      Additional Golimumab Antibody Formats

      Formats Clone Applications Sizes available
      Golimumab Antibody : Purified AbD20893 E 0.1 mg
      • Copyright © 2016 Bio-Rad

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse anti Penta Histidine Tag:HRPMCA5995P125 µlE, WB
        MCA5995P

        Recommended Negative Isotype Control

          Useful Reagents

            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Human anti Golimumab (Drug/Target Complex)HCA2450.1 mgE
            HCA245
            Hispec Assay DiluentBUF049A50 mlIY
            BUF049A

            Recommended Positive Controls

              Histology Controls

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