Generation of Different Types of Anti-Drug Antibodies

Different Binding Modes of Anti-Biotherapeutic Antibodies

HuCAL guided selection strategies allow the development of three types of anti-biotherapeutic antibodies.


Type 1

Type 2

Type 3

  • Anti-idiotypic (anti-ID) antibody
  • Paratope-specific
  • Inhibitory
  • Neutralizing
  • Detects free drug
  • Anti-idiotypic antibody
  • Not paratope-specific
  • Not inhibitory
  • Detects total drug (free, partially bound, fully bound)
  • Drug-target complex specific
  • Not inhibitory
  • Detects bound drug exclusively

Ready-Made Anti-Biotherapeutic Antibody Range

  Binding Modes Available
Antigen Type 1 Type 2 Type 3
Adalimumab Yes   Yes
Alemtuzumab Yes    
Bevacizumab Yes    
Cetuximab Yes Yes  
Golimumab Yes Yes Yes
Infliximab Yes Yes  
Natalizumab  Yes    
Omalizumab Yes Yes  
Palivizumab Yes    
Panitumumab Yes    
Rituximab Yes    
Tocilizumab Yes Yes  
Trastuzumab Yes   Yes
Ustekinumab Yes    

Custom Anti-Biotherapeutic Antibody Generation

The creation of highly specific HuCAL® custom anti-biotherapeutic Fab antibodies is accomplished in as little as 8 weeks. Clients receive purified, specificity-tested custom Fab antibodies for further characterization. Selected candidate Fab antibodies can then be converted into full-length human immunoglobulins. Purified human immunoglobulins of the chosen isotype can be delivered 12 weeks after initial generation of the Fab antibodies.

Choose from a range of human isotypes when preparing your fully human reference standards: IgA, IgE, IgG1, IgG2, IgG3, IgG4 and IgM.

anti-drug antibody generation process

Figure 2: Process Overview for Generation of HuCAL Anti-Drug Antibodies

HuCAL® is a registered trademark of MorphoSys AG.