Tyrosinase Antibody

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Mushroom tyrosinase detected with Sheep anti Mushroom Tyrosinase (9344-9979G)

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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    9344-9979E, WBdatasheet pdfdatasheet pdf0.2 ml
    9344-9979
    9344-9979GE, WBdatasheet pdfdatasheet pdf1 ml
    9344-9979G
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • 9344-9979G is specific for mushroom Tyrosinase.
    • Intended Use
    • Target Species
      Fungal
    • Product Form
      Purified IgG - liquid
      Serum - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Purified native mushroom tyrosinase
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 5mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      oxidation-reduction process
      metal ion binding
      monophenol monooxygenase activity
      melanin biosynthetic process
    • UniProt
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/5001/5000
      Western Blotting
    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Western Blotting

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
      In a simple ELISA with 1 µg of antigen coated per well, the 50% OD was observed at 1/20,000. Under the same conditions, the end point was observed at >1/400,000. Alkaline phosphatase conjugated anti-sheep was used for detection. All incubations at 18ºC using pNPP substrate, with OD measurements at 405 nm.
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional Tyrosinase Antibody Formats

    Formats Applications Sizes available
    Tyrosinase Antibody : Purified E, WB 1 ml
    Tyrosinase Antibody : Serum E, WB 0.2 ml
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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