Tyrosinase Antibody

//www.bio-rad-antibodies.com/fungal-tyrosinase-antibody-9344-9979g.html?RenderPageSpecific=1
Tyrosinase Antibody gallery image 1

Mushroom tyrosinase detected with Sheep anti Mushroom Tyrosinase (9344-9979G)

Enlarge
  • Tyrosinase Antibody thumbnail image 1
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    9344-9979E, WBdatasheet pdfdatasheet pdf0.2 ml
    9344-9979
    9344-9979GE, WBdatasheet pdfdatasheet pdf1 ml
    9344-9979G
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Sheep anti Mushroom tyrosinase antibody recognizes mushroom tyrosinase, also known as 5,6-dihydroxyindole-2-carboxylic acid oxidase, DHICA oxidase, Glycoprotein 75, Melanoma antigen gp75, Tyrosinase-related protein 1 or TRP-1. Tyrosinase is widely distributed in both antmals and plants, it is a copper containing oxidase involved in the biosynthesis of melanin in mammals and is involved in fruit browning reactions (Thalmann and Lötzbeyer 2002).

      In man, mutations in the TYRP1 gene can lead to the development of Albinism, oculocutaneous, 3 (OCA3), a condition characterized by reduced deposition of melanin in the skin, hair and eyes.
    • Intended Use
    • Target Species
      Fungal
    • Product Form
      Purified IgG - liquid
      Serum - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Purified native mushroom tyrosinase
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 5mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      oxidation-reduction process
      metal ion binding
      monophenol monooxygenase activity
      melanin biosynthetic process
    • UniProt
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/5001/5000
      Western Blotting
    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Western Blotting

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
      In a simple ELISA with 1 µg of antigen coated per well, the 50% OD was observed at 1/20,000. Under the same conditions, the end point was observed at >1/400,000. Alkaline phosphatase conjugated anti-sheep was used for detection. All incubations at 18ºC using pNPP substrate, with OD measurements at 405 nm.
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional Tyrosinase Antibody Formats

    Formats Applications Sizes available
    Tyrosinase Antibody : Purified E, WB 1 ml
    Tyrosinase Antibody : Serum E, WB 0.2 ml
    • Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Sheep IgG (H/L):Biotin5184-23041.5 mlC, E, F, WB
      5184-2304
      Donkey anti Sheep/Goat IgG:DyLight®488STAR88D488GA0.1 mgF, IF
      STAR88D488GA
      Donkey anti Sheep/Goat IgG:DyLight®549STAR88D549GA0.1 mgF, IF
      STAR88D549GA
      Donkey anti Sheep/Goat IgG:DyLight®649STAR88D649GA0.1 mgF, IF
      STAR88D649GA
      Donkey anti Sheep/Goat IgG:FITCSTAR88F1 mgC
      STAR88F
      Donkey anti Sheep/Goat IgG:HRPSTAR88P1 mlC, E, P, WB
      STAR88P
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Sheep IgG (H/L):Biotin5184-23041.5 mlC, E, F, WB
      5184-2304
      Donkey anti Sheep/Goat IgG:DyLight®488STAR88D488GA0.1 mgF, IF
      STAR88D488GA
      Donkey anti Sheep/Goat IgG:DyLight®549STAR88D549GA0.1 mgF, IF
      STAR88D549GA
      Donkey anti Sheep/Goat IgG:DyLight®649STAR88D649GA0.1 mgF, IF
      STAR88D649GA
      Donkey anti Sheep/Goat IgG:FITCSTAR88F1 mgC
      STAR88F
      Donkey anti Sheep/Goat IgG:HRPSTAR88P1 mlC, E, P, WB
      STAR88P

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

              Write your review

              You may also be interested in...