Fluorescein Conjugation Kit

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Fluorescein Conjugation Kit gallery image 1

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LYNX Rapid Fluorescein Antibody Conjugation Kit used for conjugation of anti Cyp11a1 antibody to fluoriscein and subsequent use in flow cytometry
Image caption:
The Steroid Synthesis Pathway in Th2 Cells Produces Pregnenolone: Steroid Production In Vitro during Th2 Polarization and In Vivo, in Response to Helminth Infection: (A) Quantitative detection of pregnenolone by LC-MS/MS. Naive Th cells were cultured in Th1 or Th2 activation-differentiation conditions. After 3 days of stimulation, cells were rested for 2 days with equal cell density. Cell supernatants were extracted for steroid profiling. Bars represent the mean pregnenolone concentration ±SEM. p values were calculated by unpaired two-tailed t test. (B) Quantitative detection of pregnenolone by competitive ELISA. Th cells were stimulated for 3 days and rested for 3 days before supernatants were analyzed by competitive ELISA. Bars represent the mean concentration ±SEM from four independent experiments. p values were calculated using the unpaired two-tailed t test. (C) Th2 supernatants were analyzed by ELISA for pregnenolone production as described in (B) with or without the presence of Cyp11a1 inhibitor, aminoglutethimide (AG). p values were calculated by unpaired two-tailed t test. Error bars are SDs from the mean. (D) Schematic flowchart of in vivo experimental design. C57BL/6 mice were infected with nematode larvae (Nippostrongylus brasiliensis) for 10 days. On day 5 and day 10 postinfection, CD4+ Th cells were purified from spleens and mesenteric lymph nodes (MLN). They were then immediately analyzed by FACS for Cyp11a1 expression, or ex vivo cultured for 24 hr for detection of pregnenolone production by ELISA. (E) Cyp11a1 and GATA3 expression detected by FACS. Data shown were obtained from pooled samples of spleen and MLN of five mice and represent two experiments (n = 5 and n = 3 for each condition). p values were calculated using the unpaired two-tailed t test using data obtained from individual mice (n = [5 + 3] = 8). (F) Pregnenolone concentration of ex vivo cultured Th cell supernatant was measured by quantitative ELISA. Results shown are mean pregnenolone concentration ±SD from both spleen and MLN. Experiments were performed twice with eight mice per condition. p values were calculated using unpaired two-tailed t test.

From: Mahata B, Zhang X, Kolodziejczyk AA, Proserpio V, Haim-Vilmovsky L, Taylor AE, Hebenstreit D, Dingler FA, Moignard V, Göttgens B, Arlt W, McKenzie AN, Teichmann SA. Single-cell RNA sequencing reveals T helper cells synthesizing steroids de novo to contribute to immune homeostasis. Cell Rep. 2014 May 22;7(4):1130-42.

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Fluorescein Conjugation Kit gallery image 2

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LYNX Rapid Fluorescein Antibody Conjugation Kit used for conjugation of anti Cyp11a1 antibody to fluoriscein and subsequent use in flow cytometry
Image caption:
Gene Expression Identity of Cyp11a1-Expressing Th2 Cells and Ly6C as a Surface Marker for Steroidogenic Cell Purification: (A) Schematic summary of Cyp11a1-correlated genes as obtained from mRNA sequencing analysis of 91 single Th2 cells (52 cells from G4P and 39 cells from G2N, as shown in the Figure 3A). Genes are listed according to their correlation coefficient value, higher to lower, top to bottom in each category. Genes previously reported to be involved in immunosuppression are red and genes not coexpressed are crossed out. RNA sequencing and analysis are as described in Figures S3 and S4 and Tables S2, S3, and S4. (B) IL-4 mRNA expression level was plotted against Cyp11a1 mRNA expression as obtained from single-cell RNA sequencing. Each dot represents an individual cell. The color of a dot varies from yellow to black, representing the mRNA expression level of Gata3 in the cell. (C) Normalized mRNA expression level of Ly6C1 and Ly6C2 plotted against Cyp11a1 mRNA expression. Each dot represents an individual cell. (D) Ly6C+ cells were FACS sorted from in vitro differentiated Th1 (left panel) and Th2 (right panel) cell populations and FACS analyzed for Cyp11a1. Purified Th1 cells have a trace proportion of Cyp11a1+ cells, whereas almost all purified Th2 cells are Cyp11a1+. (E) Ly6C+ cells were FACS sorted from in vitro differentiated Th2 population and FACS analyzed for the expression of GATA3, FOXP3, and Cyp11a1.

From: Mahata B, Zhang X, Kolodziejczyk AA, Proserpio V, Haim-Vilmovsky L, Taylor AE, Hebenstreit D, Dingler FA, Moignard V, Göttgens B, Arlt W, McKenzie AN, Teichmann SA. Single-cell RNA sequencing reveals T helper cells synthesizing steroids de novo to contribute to immune homeostasis. Cell Rep. 2014 May 22;7(4):1130-42.

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Fluorescein Conjugation Kit gallery image 3

Published customer image:
LYNX Rapid Fluorescein Antibody Conjugation Kit used for conjugation of anti Cyp11a1 antibody to fluoriscein and subsequent use in flow cytometry
Image caption:
Gene expression identity of Cyp11a1-expressing Th2 cells (A,B,C) Cyp11a1 clusters with Th2 and suppressor genes and Cyp11a1+ cells cluster. (A) Hierarchical clustering on the Spearman correlation coefficient matrix of the 175 selected immunity genes (listed in Table S1) in all 91 cells. A large cluster includes Th2 genes, and a more compact cluster includes Cyp11a1 and many suppressor genes. (B) Hierarchical clustering on Spearman correlation coefficient matrix of the 175 genes in 52 G4P Th2 cells (4th generation and positive for IL13-GFP, Figure S4). A large cluster includes Th2 genes, and a more compact cluster includes Cyp11a1 with many suppressor genes. (C) Hierarchical clustering on Spearman correlation matrix of cells based on 78 genes: 46 genes negatively correlated with Cyp11a1 (Spearman correlation < -0.35), and 32 genes positively correlated with Cyp11a1 (Spearman correlation > 0.35). The leftmost cluster (black box) of cells corresponds to those with high Cyp11a1 expression. (D, E) Ly6C+ Th2 cells are steroidogenic and do not express type-1 regulatory (Tr1) markers LAG3 and CD49b. (D) In vitro polarized Th cells (3 days activated, 2 days resting) were FACS analyzed for Ly6C and Cyp11a1. (E) In vitro polarized Th2 cells (3 days activated, 2 days resting) were analyzed for the presence of Ly6C, CD49b and LAG3 compared to the isotype control.

From: Mahata B, Zhang X, Kolodziejczyk AA, Proserpio V, Haim-Vilmovsky L, Taylor AE, Hebenstreit D, Dingler FA, Moignard V, Göttgens B, Arlt W, McKenzie AN, Teichmann SA. Single-cell RNA sequencing reveals T helper cells synthesizing steroids de novo to contribute to immune homeostasis. Cell Rep. 2014 May 22;7(4):1130-42.

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LYNX Rapid Fluorescein Antibody Conjugation Kit used for conjugation of anti Cy5 antibody and subsequent blotting of membranes and immunofluorescence
Image caption:
Cathepsin-L-induced unquenching of GB119 increases availability of Cy5 in GB119 for anti-Cy5 antibody in vitro: a) Assay Validation. Donkey IgG labeled with Cy5 (Dky IgG/Cy5) was adhered to nitrocellulose membrane (NCM) and then probed with Anti-Cy5 antibodies that were untreated (Anti-Cy5 Ab) or pre-adsorbed with donkey Dky IgG/Cy5 (Anti-Cy5 Ab, Dky IgG/Cy5). Data was normalized to average signal measured when the anti-Cy5 was not pre-adsorbed. Color inset represents typical signal measured from the nitrocellulose using the Maestro imaging device. b) Pre-adsorption of anti-Cy5 Ab with GB119. Dky IgG/Cy5 was adhered to NCM and then probed with i) anti-Cy5 antibody pre-incubated with quenched GB119 (Anti-Cy5, GB119); ii) anti-Cy5 antibody pre-incubated with GB119 that had first been unquenched with human cathepsin-L (CTS-L) (Anti-Cy5Ab, GB119, CTS-L); and iii) anti-Cy5 antibody pre-incubated with GB119 that had been unquenched with human CTS-L in the presence of a general papain family protein inhibitor, JMP-OEt (PI), i.e. negative control, (Anti-Cy5 Ab, GB119, CTS-L, PI). Color inset represents typical signal measured from the nitrocellulose using the Maestro imaging device. c) Pseudo green color images of anti-Cy5 antibody fluorescence from NCM blots of donkey antibody labeled with Cy5 as in (b) that were taken by Leica microscope (1.25x) indicating differential is suitable for visualization of unquenched GB119 in tissue. d) Pre-washed blots of GB119 on NCM were incubated with i) CTS-L alone, ii) PBS alone, or as controls a mixture of iii) CTS-L and an excess of a PI, or iv) a mixture of PBS and the PI. Fluorescent images indicate strong unquenching of GB119 by CTS-L only in the absence of inhibitor. PBS alone did not unquench GB119. e) Quantitative analysis of levels of Cy5 fluorescence. f) Nitrocellulose membranes from inset (2d) were probed with fluorescein-labeled anti-Cy5 antibodies and imaged for fluorescence. Results were similar to those determine in inset (d). g) Quantitative analysis of levels of anti-Cy5 Ab fluorescence. For a) the difference in signal was significant, p<0.001; for b) the difference in signal was compared to anti-Cy5Ab, GB119 and was significant for both cases, p<0.001 and p<0.05, respectively. Statistical data (e) and (g) are presented as mean levels of fluorescence from which matched PBS only controls were subtracted. For d) p<0.001; for g) p<0.05. Cy5 fluorescence (emission filter = 645 nm); Fluorescence of anti-Cy5 Ab (emission filter = 515 nm). Error bars represent +SD.

From: Walker E, Gopalakrishnan R, Bogyo M, Basilion JP. Microscopic detection of quenched activity-based optical imaging probes using an antibody detection system: localizing protease activity. Mol Imaging Biol. 2014 Oct;16(5):608-18.

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LYNX Rapid Fluorescein Antibody Conjugation Kit used for conjugation of anti Cy5 antibody and subsequent blotting of membranes and immunofluorescence
Image caption:
Tumor lysate unquenching of GB119 is detectable using anti-Cy5 antibodies: A constant amount of GB119 was spotted onto nitrocellulose filters, and then incubated with either rabbit polyclonal IgG (control) or increasing amounts of cell lysate derived from a human Gli36?5 cancer cell line to unquench GB119. Nitrocellulose membranes were imaged for: a) Cy5 fluorescence or b) anti-Cy5 antibody signal (fluorescein). Correlation between the signal from direct Cy5 fluorescence and anti-Cy5 detected GB119 unquenching was statistically correlated in inset (c). Vertical bars (a) and (b) represent mean fluorescence +SD for each point of lysate concentration. Spearman rank correlation test was used (r= 0.9; p< 0.01) to analyze relationship between levels of two types of fluorescence (c).

From: Walker E, Gopalakrishnan R, Bogyo M, Basilion JP. Microscopic detection of quenched activity-based optical imaging probes using an antibody detection system: localizing protease activity. Mol Imaging Biol. 2014 Oct;16(5):608-18.

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LYNX Rapid Fluorescein Antibody Conjugation Kit used for conjugation of anti Cy5 antibody and subsequent blotting of membranes and immunofluorescence
Image caption:
Unquenching of GB119 can be detected by immunohistochemical analysis of tissue sections The brain section from Fig. 4 was cut perpendicular to the plane of the section and indicated in inset (a) and underwent IHC analysis. b) H&E staining of the sections derived from the cut described in inset (a). The tumor is clearly visible – black asterisk. c) Anti-Cy5 antibody staining for unquenched GB119 of an adjacent section of tissue. Gray arrows highlight anti-Cy5 staining, green. The dotted white line in inset (a) approximates the edges of the tissue slice, which are overlapped by the probe applicator. White asterisk – approximate the visible portion of the tumor xenograft (see Fig. 4); scale bar – 500 µm. d) Histology image (b) and image of IHC staining for Cy5 (c) overlay. Red arrowheads indicate location of topically applied GB119.

From: Walker E, Gopalakrishnan R, Bogyo M, Basilion JP. Microscopic detection of quenched activity-based optical imaging probes using an antibody detection system: localizing protease activity. Mol Imaging Biol. 2014 Oct;16(5):608-18.

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Fluorescein Conjugation Kit gallery image 7

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LYNX Rapid Fluorescein Antibody Conjugation Kit used for conjugation of anti Cy5 antibody and subsequent blotting of membranes and immunofluorescence
Image caption:
Unquenching of GB119 localizes in the peri-tumor space and is associated with tumor cells, macrophages, and the expression of cathepsin-L The brain section from Fig. 4 was cut perpendicular to the plane of the section and indicated in inset (a) and underwent immunohistochemical analysis. b) H&E staining of the sections derived from the cut described in inset (a). Boxed regions correspond to the approximate area for the sections in inset (c). c) Frames 1–8: Immunological and H&E staining corresponding to regions 1–8 boxed in inset (b). Images are composites of three stains: Anti-Cy5 antibody staining (false red), cathepsin-L (CTS-L) (false green), and presence or absence of the cancerous tissue (vimentin – false blue). The yellow-orange color represents areas of Cy5/CTS-L co-registration along the tumor edge and on the surface of the sample. Purple indicates CTS-L and vimentin co-localization. Frame 9 (green box): H&E and IHC staining overlay derived from an adjacent section of the tumor area depicted as a green box in inset (b). Overlay of staining demonstrate that macrophages and unquenched GB119 co-localize, but that some macrophages are not localized with unquenched GB119. In non-tumor areas no macrophages were found (data not shown). Unquenched GB119 (false red), tumor tissue (vimentin staining – false blue) and macrophages (CD11b staining – false green). The yellow-orange color represents areas of Cy5/CD11b (macrophages) co-registration. Black dotted lines – approximate the edge of the tumor xenograft. Red arrowheads indicate surface where the probe was topically applied and direction of the probe penetration; scale bars =100 µm.

From: Walker E, Gopalakrishnan R, Bogyo M, Basilion JP. Microscopic detection of quenched activity-based optical imaging probes using an antibody detection system: localizing protease activity. Mol Imaging Biol. 2014 Oct;16(5):608-18.

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  • LYNX Rapid Fluorescein Antibody Conjugation Kit
  • LYNX Rapid Fluorescein Antibody Conjugation Kit
  • LYNX Rapid Fluorescein Antibody Conjugation Kit
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  • Product Type
    Conjugation Kit
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    LNK062FCJdatasheet pdfdatasheet pdf datasheet pdf datasheet pdf1 Conjugation For 2mg Antibody
    LNK062F
    LNK063FCJdatasheet pdfdatasheet pdf datasheet pdf datasheet pdf3 Conjugations For 20µg Antibody
    LNK063F
    LNK061FCJdatasheet pdfdatasheet pdf datasheet pdf datasheet pdf3 Conjugations For 200µg Antibody
    LNK061F
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • LYNX Rapid Fluorescein Antibody Conjugation Kit® enables the rapid conjugation of a pre-prepared lyophilized mixture containing Fluorescein label to an antibody or protein. Activation of proprietary reagents within the antibody-label solution results in directional covalent bonding of Fluorescein to the antibody.

      The LYNX Rapid Conjugation kit® can be used to label small quantities of antibody/protein at near neutral pH, allowing a high conjugation efficiency with 100% antibody recovery.
    • Intended Use
    • Product Form
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
      Pack Size: 3 Conjugations For 200µg Antibody, 3 Conjugations For 20µg Antibody3 Vials LYNX lyophilized Fluorescein mix
      1 Vial LYNX Modifier reagent
      1 Vial LYNX Quencher reagent
      Pack Size: 1 Conjugation For 2mg Antibody1 Vial LYNX lyophilized Fluorescein mix
      1 Vial LYNX Modifier reagent
      1 Vial LYNX Quencher reagent
      Pack Size: 3 Conjugations For 200µg Antibody, 3 Conjugations For 20µg Antibody3 Vials LYNX lyophilized Fluorescein mix
      1 Vial LYNX Modifier reagent
      1 Vial LYNX Quencher reagent
      Pack Size: 1 Conjugation For 2mg Antibody1 Vial LYNX lyophilized Fluorescein mix
      1 Vial LYNX Modifier reagent
      1 Vial LYNX Quencher reagent
      Pack Size: 3 Conjugations For 200µg Antibody, 3 Conjugations For 20µg Antibody3 Vials LYNX lyophilized Fluorescein mix
      1 Vial LYNX Modifier reagent
      1 Vial LYNX Quencher reagent
      Pack Size: 1 Conjugation For 2mg Antibody1 Vial LYNX lyophilized Fluorescein mix
      1 Vial LYNX Modifier reagent
      1 Vial LYNX Quencher reagent
    • Preparing The Antibody
      Pack Size: 3 Conjugations For 200µg AntibodyThe following buffer solutions are recommended:for preparing the antibody:

      10-50mM amine-free buffer (e.g HEPES, MES, MOPS and phosphate) pH range 6.5-8.5, although moderate concentrations of Tris buffer (<20mM) may be tolerated.

      If possible, avoid buffers containing nucleophilic components such as primary amines and thiols (e.g. thiomersal/thimerosal) since they may react with LYNX chemicals. Azide (0.02-0.1%), EDTA and common non-buffering salts and sugars have little or no effect on conjugation efficiency.

      It is recommended that 100-200ug antibody be used in each labelling reaction. For optimal results the antibody volume should be 40-100ul, at a concentration range of 1-4mg/ml.
      Pack Size: 1 Conjugation For 2mg AntibodyThe following buffer solutions are recommended:for preparing the antibody:

      10-50mM amine-free buffer (e.g HEPES, MES, MOPS and phosphate) pH range 6.5-8.5, although moderate concentrations of Tris buffer (<20mM) may be tolerated.

      If possible, avoid buffers containing nucleophilic components such as primary amines and thiols (e.g. thiomersal/thimerosal) since they may react with LYNX chemicals. Azide (0.02-0.1%), EDTA and common non-buffering salts and sugars have little or no effect on conjugation efficiency.

      It is recommended that 1-2mg antibody be used in each labelling reaction. For optimal results the antibody volume should be 400-1000ul, at a concentration range of 1-4mg/ml.
      Pack Size: 3 Conjugations For 20µg AntibodyThe following buffer solutions are recommended:for preparing the antibody:

      10-50mM amine-free buffer (e.g HEPES, MES, MOPS and phosphate) pH range 6.5-8.5, although moderate concentrations of Tris buffer (<20mM) may be tolerated.

      If possible, avoid buffers containing nucleophilic components such as primary amines and thiols (e.g. thiomersal/thimerosal) since they may react with LYNX chemicals. Azide (0.02-0.1%), EDTA and common non-buffering salts and sugars have little or no effect on conjugation efficiency.

      It is recommended that 10-20ug antibody be used in each labelling reaction. For optimal results the antibody volume should be 4-10ul, at a concentration range of 1-4mg/ml.
      Pack Size: 3 Conjugations For 200µg AntibodyThe following buffer solutions are recommended:for preparing the antibody:

      10-50mM amine-free buffer (e.g HEPES, MES, MOPS and phosphate) pH range 6.5-8.5, although moderate concentrations of Tris buffer (<20mM) may be tolerated.

      If possible, avoid buffers containing nucleophilic components such as primary amines and thiols (e.g. thiomersal/thimerosal) since they may react with LYNX chemicals. Azide (0.02-0.1%), EDTA and common non-buffering salts and sugars have little or no effect on conjugation efficiency.

      It is recommended that 100-200ug antibody be used in each labelling reaction. For optimal results the antibody volume should be 40-100ul, at a concentration range of 1-4mg/ml.
      Pack Size: 1 Conjugation For 2mg AntibodyThe following buffer solutions are recommended:for preparing the antibody:

      10-50mM amine-free buffer (e.g HEPES, MES, MOPS and phosphate) pH range 6.5-8.5, although moderate concentrations of Tris buffer (<20mM) may be tolerated.

      If possible, avoid buffers containing nucleophilic components such as primary amines and thiols (e.g. thiomersal/thimerosal) since they may react with LYNX chemicals. Azide (0.02-0.1%), EDTA and common non-buffering salts and sugars have little or no effect on conjugation efficiency.

      It is recommended that 1-2mg antibody be used in each labelling reaction. For optimal results the antibody volume should be 400-1000ul, at a concentration range of 1-4mg/ml.
      Pack Size: 3 Conjugations For 20µg AntibodyThe following buffer solutions are recommended:for preparing the antibody:

      10-50mM amine-free buffer (e.g HEPES, MES, MOPS and phosphate) pH range 6.5-8.5, although moderate concentrations of Tris buffer (<20mM) may be tolerated.

      If possible, avoid buffers containing nucleophilic components such as primary amines and thiols (e.g. thiomersal/thimerosal) since they may react with LYNX chemicals. Azide (0.02-0.1%), EDTA and common non-buffering salts and sugars have little or no effect on conjugation efficiency.

      It is recommended that 10-20ug antibody be used in each labelling reaction. For optimal results the antibody volume should be 4-10ul, at a concentration range of 1-4mg/ml.
      Pack Size: 3 Conjugations For 200µg AntibodyThe following buffer solutions are recommended:for preparing the antibody:

      10-50mM amine-free buffer (e.g HEPES, MES, MOPS and phosphate) pH range 6.5-8.5, although moderate concentrations of Tris buffer (<20mM) may be tolerated.

      If possible, avoid buffers containing nucleophilic components such as primary amines and thiols (e.g. thiomersal/thimerosal) since they may react with LYNX chemicals. Azide (0.02-0.1%), EDTA and common non-buffering salts and sugars have little or no effect on conjugation efficiency.

      It is recommended that 100-200ug antibody be used in each labelling reaction. For optimal results the antibody volume should be 40-100ul, at a concentration range of 1-4mg/ml.
      Pack Size: 1 Conjugation For 2mg AntibodyThe following buffer solutions are recommended:for preparing the antibody:

      10-50mM amine-free buffer (e.g HEPES, MES, MOPS and phosphate) pH range 6.5-8.5, although moderate concentrations of Tris buffer (<20mM) may be tolerated.

      If possible, avoid buffers containing nucleophilic components such as primary amines and thiols (e.g. thiomersal/thimerosal) since they may react with LYNX chemicals. Azide (0.02-0.1%), EDTA and common non-buffering salts and sugars have little or no effect on conjugation efficiency.

      It is recommended that 1-2mg antibody be used in each labelling reaction. For optimal results the antibody volume should be 400-1000ul, at a concentration range of 1-4mg/ml.
      Pack Size: 3 Conjugations For 20µg AntibodyThe following buffer solutions are recommended:for preparing the antibody:

      10-50mM amine-free buffer (e.g HEPES, MES, MOPS and phosphate) pH range 6.5-8.5, although moderate concentrations of Tris buffer (<20mM) may be tolerated.

      If possible, avoid buffers containing nucleophilic components such as primary amines and thiols (e.g. thiomersal/thimerosal) since they may react with LYNX chemicals. Azide (0.02-0.1%), EDTA and common non-buffering salts and sugars have little or no effect on conjugation efficiency.

      It is recommended that 10-20ug antibody be used in each labelling reaction. For optimal results the antibody volume should be 4-10ul, at a concentration range of 1-4mg/ml.
    • Test Principle
    • Buffer Solution
    • Storage
      Store kit at -20oC only.
      Newly-conjugated antibody can be stored at 4oC. For long term storage however, the addition of a preservative is recommended.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted.
      Avoid repeated freezing and thawing.
      Store kit at -20oC only.
      Newly-conjugated antibody can be stored at 4oC. For long term storage however, the addition of a preservative is recommended.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted.
      Avoid repeated freezing and thawing.
      Store kit at -20oC only.
      Newly-conjugated antibody can be stored at 4oC. For long term storage however, the addition of a preservative is recommended.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted.
      Avoid repeated freezing and thawing.
    • Shelf Life
      12 months after despatch
      12 months after despatch
      12 months after despatch
    • Acknowledgements
    • Licensed Use
      These products and the methodology of conjugation are patent protected under United Kingdom patent number 2446088 and associated international patent applications. The purchase of this product conveys to the buyer the limited, non exclusive non-transferable right (without the right to resell repackage or further sublicense) under these patents to use the product to make conjugates for research and development purposes only. The purchaser cannot sell or otherwise transfer this product, or its components, or materials or data made using this product, or its components to a third party. Further information on purchasing licenses for diagnostic and other uses may be obtained by contacting Bio-Rad, at. Endeavour House, Langford Business Park, Langford Lane, Kidlington, Oxon. OX5 1GE UNITED KINGDOM. Tel: +44 1865 852 700. E-mail: antibodies@bio-rad.com
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Conjugation
    • Application NameYesNoMin DilutionMax Dilution
      Conjugation
    • Application NameYesNoMin DilutionMax Dilution
      Conjugation


    • We recommend that for each conjugation the user determines the best antibody:conjugate ratio.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
      1. To the antibody sample add 1ul of the Modifier reagent for every 10ul of antibody and mix gently.

      2. Pipette the mixed antibody-modifier sample directly onto the LYNX lyophilized mix and gently pipette up and down twice to resuspend.

      3. Replace cap onto vial and incubate in the dark at room temperature (20-25oC) overnight.

      4. After incubation, add 1ul of Quencher reagent for every 10ul of antibody used. Leave to stand for 30 minutes before use.

    • Instructions For Use
      1. To the antibody sample add 1ul of the Modifier reagent for every 10ul of antibody and mix gently.

      2. Pipette the mixed antibody-modifier sample directly onto the LYNX lyophilized mix and gently pipette up and down twice to resuspend.

      3. Replace cap onto vial and incubate in the dark at room temperature (20-25oC) overnight.

      4. After incubation, add 1ul of Quencher reagent for every 10ul of antibody used. Leave to stand for 30 minutes before use.

    • Instructions For Use
      1. To the antibody sample add 1ul of the Modifier reagent for every 10ul of antibody and mix gently.

      2. Pipette the mixed antibody-modifier sample directly onto the LYNX lyophilized mix and gently pipette up and down twice to resuspend.

      3. Replace cap onto vial and incubate in the dark at room temperature (20-25oC) overnight.

      4. After incubation, add 1ul of Quencher reagent for every 10ul of antibody used. Leave to stand for 30 minutes before use.

    Additional Fluorescein Conjugation Kit Formats

    Formats Applications Sizes available
    Fluorescein Conjugation Kit : Kit CJ 1 Conjugation For 2mg Antibody | 3 Conjugations For 20µg Antibody | 3 Conjugations For 200µg Antibody
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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