FITC Antibody

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FITC Antibody gallery image 1

Published customer image:
HRP conjugated Sheep anti FITC antibody (640005) used for the detection of FITC labeled LPS by ELISA
Image caption:
Comparison of LPS binding in indirect ELISA with different buffers and blocking agents.
FITC-LPS from E. coli serotype O111:B4 was incubated at 1.25 μg/ml in PBS-EDTA (PBS), with or without 0.05% Tween 20 (PBS-TW2) or Triton X-100 (PBS-TTX), in wells coated with polymyxin B (PMX) or lysozyme (LSZ) (1 μg/well), or empty wells (control), which were previously blocked with BSA (A) or OVA (B). Each assay was performed in triplicate. Mean values ± SD are shown in the graph.

From: Martínez-Sernández V, Orbegozo-Medina RA, Romarís F, Paniagua E, Ubeira FM (2016)
Usefulness of ELISA Methods for Assessing LPS Interactions with Proteins and Peptides.
PLoS ONE 11(6): e0156530.

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FITC Antibody gallery image 2

Published customer image:
HRP conjugated Sheep anti FITC antibody (640005) used for the detection of FITC labeled LPS in a competitive hCAS ELISA
Image caption:
Competitive hCAS-ELISA performed under neutral conditions.
FITC-LPSs (5 μg/ml) from E. coli serotypes O111:B4 (A) and O55:B5 (B) were preincubated with different concentrations of proteins and peptides (40, 10, 1 and 0.25 μg/ml) in PBS-EDTA (pH 7.2) and added to the wells coated with casein hydrolysate (hCAS). Data are expressed as percentage inhibition of LPS binding to hCAS by the target molecule and are the mean values ±SD of duplicate wells. The average values of the optical density (492 nm) of control wells (without inhibitor) were 0.311 (±0.019, red dashed line) and 0.300 (±0.025, red dashed line) for serotypes O111:B4 and O55:B5, respectively. Differences were considered significant at p <0.05. NS: not significant. BSA, bovine serum albumin; HB, hemoglobin; HF1, histone f1 fraction; LF, lactoferrin; LSZ, lysozyme; MEL, melittin; MF6p, synthetic FhHDM-1/MF6p; MYO, myoglobin; P3L, A. simplex peptide: MCQCVQKYGTEFCKKRLA; PMX, polymyxin B.

From: Martínez-Sernández V, Orbegozo-Medina RA, Romarís F, Paniagua E, Ubeira FM (2016)
Usefulness of ELISA Methods for Assessing LPS Interactions with Proteins and Peptides.
PLoS ONE 11(6): e0156530.

Enlarge
FITC Antibody gallery image 3

Published customer image:
HRP conjugated Sheep anti FITC antibody (640005) used for the detection of FITC labeled LPS in a competitive PMX ELISA
Image caption:
Competitive PMX-ELISA.
FITC-LPSs from E. coli serotypes O111:B4 (A) and O55:B5 (B) were preincubated, at 1.25 and 2.5 μg/ml, respectively, with three different concentrations of proteins and peptides (10, 1 and 0.25 μg/ml) in PBS-EDTA with 0.1% BSA and added to wells containing biotinylated polymyxin B captured by deglycosylated avidin. Results are expressed as percentage inhibition of LPS binding to captured polymyxin B by the target protein/peptide, and are the mean values ±SD for duplicate wells. The average optical densities (492 nm) of control wells (without inhibitor) were 0.904 (±0.042, red dashed line) and 0.842 (±0.036, red dashed line) for serotypes O111:B4 and O55:B5, respectively. Differences were considered significant at p <0.05. NS: not significant. HB, hemoglobin; HF1, histone f1 fraction; LF, lactoferrin; LSZ, lysozyme; MEL, melittin; MF6p, synthetic FhHDM-1/MF6p; MYO, myoglobin; P3L, A. simplex peptide: MCQCVQKYGTEFCKKRLA; PMX, polymyxin B.

From: Martínez-Sernández V, Orbegozo-Medina RA, Romarís F, Paniagua E, Ubeira FM (2016)
Usefulness of ELISA Methods for Assessing LPS Interactions with Proteins and Peptides.
PLoS ONE 11(6): e0156530.

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  • FITC Antibody thumbnail image 1
  • FITC Antibody thumbnail image 2
  • FITC Antibody thumbnail image 3
  • Sheep anti FITC
  • Sheep anti FITC:Alk. Phos.
  • Sheep anti FITC:HRP
  • Sheep anti FITC:RPE
  • Sheep anti FITC:Biotin
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
5 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    640009IFdatasheet pdfdatasheet pdf0.5 mg
    640009
    640001C, Edatasheet pdfdatasheet pdf1 mg
    640001
    640008C, Edatasheet pdfdatasheet pdf1 mg
    640008
    640004Cdatasheet pdfdatasheet pdf1 ml
    640004
    640005Edatasheet pdfdatasheet pdf1 ml
    640005
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Sheep anti FITC antibody recognizes the fluorochrome Fluorescein Isothiocyanate (FITC). This reagent may be useful for amplification of staining using FITC conjugated reagents.
    • Intended Use
    • Target Species
      Chemical
    • Product Form
      Purified IgG - liquid
      Purified IgG conjugated to Alkaline Phosphatase - liquid
      Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
      Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
      Purified IgG conjugated to Biotin - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography.
      Purified IgG prepared by affinity chromatography.
      Purified IgG was prepared from whole serum by affinity chromatography.
      Purified IgG prepared by affinity chromatography.
      Purified IgG prepared by affinity chromatography.
    • Antiserum Preparation
      Antiserum to FITC was raised by repeated immunisation of sheep with highly purified antigen.
    • Preservative Stabilisers
      None present
      0.1% Sodium Azide (NaN3)
      50% Glycerol
      50% Glycerol
      0.1% Sodium Azide (NaN3)
      Stabilising agent
      0.1% Sodium Azide (NaN3)
    • Immunogen
      Fluorescein isothiocyanate (isomer 1).
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
      IgG concentration 0.5 mg/ml
      IgG concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Borate buffered saline
      TRIS buffered saline, 1mM MgCl2
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. This product is photosensitive and should be protected from light.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      12 months from date of despatch.
      Please see label for expiry date.
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Immunohistology - Frozen
    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/40001/8000
    • Application NameYesNoMin DilutionMax Dilution
      Immunofluorescence
    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Immunohistology - Frozen

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional FITC Antibody Formats

    Formats Applications Sizes available
    FITC Antibody : Purified C, E 1 mg
    FITC Antibody : RPE IF 0.5 mg
    FITC Antibody : Biotin C, E 1 mg
    FITC Antibody : Alk. Phos. C 1 ml
    FITC Antibody : HRP E 1 ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

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      STAR88D488GA
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      STAR88D549GA
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      STAR88P
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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      STAR119

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          AbGUARD® HRP Stabilizer PlusBUF052A100 mlC, E, P, WB
          BUF052A
          TMB Signal+BUF054A100 mlE
          BUF054A
          TMB CoreBUF056A100 mlE
          BUF056A
          TMB Core PrestainedBUF057A100 mlE
          BUF057A
          TMB Core+BUF062A100 mlE
          BUF062A
          AbGUARD® HRP Stabilizer PlusBUF052C1000 mlC, E, P, WB
          BUF052C
          AbGUARD® HRP Stabilizer PlusBUF052B500 mlC, E, P, WB
          BUF052B

          Recommended Positive Controls

            Histology Controls

              References

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