FITC Antibody

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FITC Antibody gallery image 1

Published customer image:
Horseradish peroxidase conjugated Rabbit anti FITC antibody used as a visualization antibody in an ELISA assay for the detection of anti Fasciola hepatica antibodies in human sera.
Image caption:
Comparison of the detection limit of the MM3-SERO and SeroFluke tests. The ability of MM3-SERO ELISA (A) and SeroFluke (B) to detect anti-Fasciola antibodies in two fold dilutions of pooled positive sera was compared. The starting serum dilution was 1/100 for both tests.

From: Martínez-Sernández V, Muiño L, Perteguer MJ, Gárate T, Mezo M, González-Warleta M, et al. (2011) Development and Evaluation of a New Lateral Flow Immunoassay for Serodiagnosis of Human Fasciolosis.
PLoS Negl Trop Dis 5(11): e1376.

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  • FITC Antibody thumbnail image 1
  • Rabbit anti FITC
  • Rabbit anti FITC:HRP
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    4510-7804C, Edatasheet pdfdatasheet pdf1 mg
    4510-7804
    4510-7864C, E, WBdatasheet pdfdatasheet pdf1 ml
    4510-7864
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rabbit anti FITC antibody recognizes fluorescein and does not react with immunoglobulins from any species.

      Fluorescein isothiocyanate (FITC) is widely used to attach a fluorescent label to proteins via the amine group. The isothiocyanate group reacts with amino terminal and primary amines in proteins.
    • Intended Use
    • Target Species
      Chemical
    • Product Form
      Purified Ig - liquid
      Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by immunoaffinity chromatography
      Purified IgG prepared by affinity chromatography.
    • Preservative Stabilisers
      0.1%Sodium Azide (NaN3)
      0.02%Thiomersal
      1% Bovine serum albumin.
    • Immunogen
      FITC Isomer 1.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
      1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Immunohistology - Frozen
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/2001/1000
      Immunohistology - Frozen1/201/200
      Western Blotting1/201/200

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional FITC Antibody Formats

    Formats Applications Sizes available
    FITC Antibody : HRP C, E, WB 1 ml
    FITC Antibody : Purified C, E 1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

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      STAR121F
      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
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      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      STAR121P
      Goat anti Rabbit IgG (H/L):HRPSTAR124P1 mgC, E, WB
      STAR124P
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      STAR54
      Sheep anti Rabbit IgG:RPESTAR35A1 mlF
      STAR35A

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          AbGUARD® HRP Stabilizer PlusBUF052A100 mlC, E, P, WB
          BUF052A
          TMB Signal+BUF054A100 mlE
          BUF054A
          TMB CoreBUF056A100 mlE
          BUF056A
          TMB Core PrestainedBUF057A100 mlE
          BUF057A
          TMB Core+BUF062A100 mlE
          BUF062A
          AbGUARD® HRP Stabilizer PlusBUF052C1000 mlC, E, P, WB
          BUF052C
          AbGUARD® HRP Stabilizer PlusBUF052B500 mlC, E, P, WB
          BUF052B

          Recommended Positive Controls

            Histology Controls

              References

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