Etanercept Antibody | AbD26183_hIgG1

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Human anti etanercept specificity ELISA

A microtiter plate was coated overnight with various antigens at a concentration of 5 μg/ml. After washing and blocking with PBST+5% BSA, detection was performed using HRP-conjugated Human anti etanercept (clone AbD26183_hIgG1) at a concentration of 2 μg/ml in HISPEC diluent (BUF049A) and QuantaBlu™ fluorogenic peroxidase substrate

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Human anti etanercept specificity titration ELISA

A microtiter plate was coated overnight with etanercept, TNFR2, Fc control or infliximab at a concentration of 1 μg/ml. After washing and blocking with PBST+5% BSA, detection was performed using HRP-conjugated human anti etanercept (clone AbD26183_hIgG1) titrated to the given concentrations in HISPEC assay diluent (BUF049A) and QuantaBlu™ fluorogenic peroxidase substrate. Data for binding to etanercept and TNFR2 are shown as the mean of three measurements

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Etanercept bridging ELISA for pharmacokinetic (PK) assay development

A microtiter plate was coated overnight with Human anti etanercept (clone AbD25939) at a concentration of 5 μg/ml. After washing and blocking with PBST+5% BSA, 10% human serum was added spiked with increasing concentrations of etanercept. Detection was performed using HRP conjugated Human anti etanercept (clone AbD26183_hIgG1) at a concentration of 2 μg/ml in HISPEC assay diluent (BUF049A) and QuantaBlu™ fluorogenic peroxidase substrate. Data are shown as the mean of three measurements. HRP-conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit®

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Human anti etanercept inhibition ELISA

A microtiter plate was coated overnight with human TNFα at a concentration of 1 μg/ml. After washing and blocking with PBST+5% BSA, a pre-incubated mixture of etanercept (0.3 μg/ml) plus increasing concentrations of Human anti etanercept (clone AbD25940_hIgG4Pro), or clone AbD26183_hIgG1, HCA279 in the monovalent Fab format was added. Free etanercept, still capable of binding to the human TNFα coated plate, was detected using Mouse anti Human IgG (Fc) CH2 Domain:HRP antibody (MCA647P) and QuantaBlu™ fluorogenic peroxidase substrate. Data are shown as the mean of three measurements

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Human anti etanercept inhibition ELISA

A microtiter plate was coated overnight with etanercept at a concentration of 1 μg/ml. After washing and blocking with PBST+5% BSA, a pre-incubated mixture of HRP conjugated Human anti etanercept (clone AbD25940_hIgG4Pro) at 2 μg/ml, or (clone AbD26183_hIgG1) at 4 μg/ml plus increasing concentrations of TNFR2 was added. Free Human anti etanercept, still capable of binding to etanercept coated plate, was detected using QuantaBlu™ fluorogenic peroxidase substrate. Data are shown as the mean of three measurements

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  • Human anti Etanercept:HRP
  • Human anti Etanercept
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    AbD26183_hIgG1
  • Isotype
    IgG1
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    HCA279PEdatasheet pdfdatasheet pdf0.1 mg
    HCA279P
    HCA279Edatasheet pdfdatasheet pdf0.1 mg
    HCA279
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Human anti etanercept, clone AbD26183_hIgG1 is an antibody that specifically recognizes etanercept at the region of the fusion of p75 with the Fc portion of human IgG1. The antibody does not inhibit the binding of etanercept to its target tumor necrosis factor alpha (TNFα); it binds to both free etanercept and to etanercept bound to its target TNFα and can be used to measure total etanercept very specifically.

      The antibody can be used to develop a pharmacokinetic (PK) bridging assay to measure total drug in patient samples. This antibody, in full immunoglobulin format, is recommended as the detection antibody, paired with the antibody clone AbD25939 in monovalent Fab format, as the capture antibody.

      Etanercept (Enbrel®) is a dimeric fusion protein consisting of the extracellular ligand-binding portion of the human 75 kDa (p75) TNFR2 linked to the Fc portion of human IgG1. The Fc component of etanercept contains the CH2 domain, the CH3 domain and hinge region, but not the CH1 domain of IgG1. It is used to treat autoimmune diseases by interfering with TNF, acting as a TNF inhibitor. It binds to both TNFα and TNFβ (Lymphotoxin A).
    • Intended Use
    • Product Form
      Human IgG1 antibody (kappa light chain) selected from the HuCAL® phage display library and expressed in a human cell line. Conjugated to horseradish peroxidase (HRP) - liquid
      Human IgG1 antibody (kappa light chain) selected from the HuCAL® phage display library and expressed in a human cell line.
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A
      Purified IgG prepared by affinity chromatography on Protein A
    • Preservative Stabilisers
      0.01% Thiomersal
      0.01% Thiomersal
    • Immunogen
      Etanercept
    • Purity
    • Affinity
      The monovalent intrinsic affinity of this antibody was measured as KD=14.8 nM by real time, label-free molecular interaction analysis on immobilized etanercept.
    • Approx. Protein Concentrations
      IgG concentration 0.1 mg/ml
      IgG concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at -70oC.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted.
    • Shelf Life
      12 months from date of despatch
      12 months from date of despatch
    • Acknowledgements
      Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany. Enbrel is a registered trademark of Immunex Corporation
      Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany. Enbrel is a registered trademark of Immunex Corporation
    • Licensed Use
      For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
    • Application NameYesNoMin DilutionMax Dilution
      ELISA

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
      This product may be used in a direct ELISA or as a detection antibody in a bridging ELISA.
      Protocol: PK bridging ELISA to measure total drug
    • ELISA
      This product may be used in a direct or indirect ELISA or as a detection antibody in a bridging ELISA together with clone AbD25939 as the capture reagent.
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional Etanercept Antibody Formats

    Formats Clone Applications Sizes available
    Etanercept Antibody : Purified AbD26183_hIgG1 E 0.1 mg
    Etanercept Antibody : HRP AbD26183_hIgG1 E 0.1 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human anti EtanerceptHCA2760.1 mgE
          HCA276
          Human anti EtanerceptHCA2790.1 mgE
          HCA279
          Hispec Assay DiluentBUF049A50 mlIY
          BUF049A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Hispec Assay DiluentBUF049A50 mlIY
          BUF049A

          Recommended Positive Controls

            Histology Controls

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