Endotoxin Removal Standard Kit

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  • Proteus MIDI Endotoxin Removal Standard Spin Column Pack
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Purification Kit
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    PUR028PPdatasheet pdf12 Units
    PUR028
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • This kit offers rapid, one step, high grade (>95%) removal of endotoxin from a wide range of protein solutions, with >90% protein recovery.

      We recommend reading the Proteus Endotoxin Removal Kit Handbook prior to use.
    • Intended Use
    • Product Form
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
      94% Preservative base of resin containing:
      20% Aqueous Ethanol
      80% 0.1M Sodium Chloride
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
      12 x Spin Column Plugs containing Endotoxin Binding Resin
      12 x Midi Standard Spin Columns (20ml capacity)
      12 x 50ml Centrifuge Tubes
      Plug Insertion Tool
      Instructions for use
    • Preparing The Antibody
    • Endotoxin Level
      This kit can be used for samples with endotoxin loads < 30,000 EU. For endotoxin loads < 3,000 EU the Mini Endotoxin Removal kit is recommended. Endotoxin loads up to 1,000,000 EU require the use of a Midi High Capacity Endotoxin Removal kit.
    • Test Principle
    • Buffer Solution
    • Additional Reagents Required
      0.2µm and 1.2µm Syringe Filter Units
      Low Endotoxin Pre-equilibration Buffer e.g PBS pH7.4
    • Storage
      Upon receipt of kit, remove resin plugs box and store this at +4oC.
      DO NOT FREEZE RESIN PLUGS OR STORE AT ROOM TEMPERATURE.
      Store remainder of kit at room temperature.
    • Shelf Life
      Please see label for expiry date.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Protein Purification

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use
      Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/PUR028.pdf

    Additional Endotoxin Removal Standard Kit Formats

    Formats Applications Sizes available
    Endotoxin Removal Standard Kit : MIDI - Spin Columns PP 12 Units
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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