Published customer image: Enlarge
Endothelial Cell Growth Supplement used to support the growth of primary endothelial cells in vitro
Cldn14 gene copy number affects endothelial cell proliferation in vivo, ex vivo and in vitro. (A) Percentages of Ki67-positive endothelial cells were counted in cryosections of 13-day B16F10 tumours from WT, Cldn14-het and Cldn14-null mice co-stained with PECAM. Endothelial cell proliferation was enhanced significantly in Cldn14-het mice. (B) Representative images of tumour sections in each genotype. Arrows, Ki67-positive endothelial cell nuclei. Scale bar 25 μm. (C) Proliferating cells in VEGF-stimulated wild-type, Cldn14-het and Cldn14-null collagen-embedded aortic explants were detected by EdU incorporation. The number of proliferating (EdU-positive) nuclei, counterstained with DAPI, was divided by the total number of cell nuclei also BS1-lectin positive to give % proliferating endothelial cells in VEGF-treated aortic rings. Bars show mean % of proliferating cells ± SEM. n = 6–8 rings per genotype, 513–717 nuclei per genotype. (D) Representative images of VEGF-stimulated WT, Cldn14-het and Cldn14-null microvessels from aortic ring explants stained for EdU and BS1 lectin. Scale bar 50 µm. (E) WT, Cldn14-het and Cldn14-null primary endothelial cells were examined for EdU incorporation in the presence of 30 ng/ml VEGF. Cells were counterstained with DAPI and the number of EdU-positive cells recorded for each genotype. Bars show mean % EdU-positive cells ± SEM. N = 1217–3464 nuclei per genotype, 3 mice per genotype. (F) Representative images of primary endothelial cells in culture. Scale bar 50 µm. Arrows, EdU-positive nuclei. NSD: no significant difference. * P<0.05, *** P<0.001.
From: Baker M, Reynolds LE, Robinson SD, Lees DM, Parsons M, Elia G, et al. (2013) Stromal Claudin14-Heterozygosity, but Not Deletion, Increases Tumour Blood Leakage without Affecting Tumour Growth.
PLoS ONE 8(5): e62516.