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Published customer image:
Endothelial Cell Growth Supplement used to support the growth of primary endothelial cells in vitro
Image caption:
Cldn14 gene copy number affects endothelial cell proliferation in vivo, ex vivo and in vitro. (A) Percentages of Ki67-positive endothelial cells were counted in cryosections of 13-day B16F10 tumours from WT, Cldn14-het and Cldn14-null mice co-stained with PECAM. Endothelial cell proliferation was enhanced significantly in Cldn14-het mice. (B) Representative images of tumour sections in each genotype. Arrows, Ki67-positive endothelial cell nuclei. Scale bar 25 μm. (C) Proliferating cells in VEGF-stimulated wild-type, Cldn14-het and Cldn14-null collagen-embedded aortic explants were detected by EdU incorporation. The number of proliferating (EdU-positive) nuclei, counterstained with DAPI, was divided by the total number of cell nuclei also BS1-lectin positive to give % proliferating endothelial cells in VEGF-treated aortic rings. Bars show mean % of proliferating cells ± SEM. n = 6–8 rings per genotype, 513–717 nuclei per genotype. (D) Representative images of VEGF-stimulated WT, Cldn14-het and Cldn14-null microvessels from aortic ring explants stained for EdU and BS1 lectin. Scale bar 50 µm. (E) WT, Cldn14-het and Cldn14-null primary endothelial cells were examined for EdU incorporation in the presence of 30 ng/ml VEGF. Cells were counterstained with DAPI and the number of EdU-positive cells recorded for each genotype. Bars show mean % EdU-positive cells ± SEM. N = 1217–3464 nuclei per genotype, 3 mice per genotype. (F) Representative images of primary endothelial cells in culture. Scale bar 50 µm. Arrows, EdU-positive nuclei. NSD: no significant difference. * P<0.05, *** P<0.001.

From: Baker M, Reynolds LE, Robinson SD, Lees DM, Parsons M, Elia G, et al. (2013) Stromal Claudin14-Heterozygosity, but Not Deletion, Increases Tumour Blood Leakage without Affecting Tumour Growth.
PLoS ONE 8(5): e62516.

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  • Endothelial Cell Growth Supplement
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  • Product Type
    Purified Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    4110-5004TCdatasheet pdfdatasheet pdf50 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Endothelial Cell Growth Supplement (ECGS), derived from bovine hypothlalmus is a beneficial media supplement for use in the following:

    • Vascular endothelial cells (human and animal), at a concentration of 100-300 μg/ml in serum supplemented media.

    • Hybridoma cell cloning, at a concentration of 25-150 μg/ml in serum supplemented media.

    • Balb/c-3T3 cells, at a concentration of 50-200 μg/ml in 5% serum supplemented media.
    • Intended Use
    • Product Form
      Purified Protein - lyophilised
    • Reconstitution
      Use 5-10 ml of sterile physiological saline. Mix gently and transfer to a sterile, screw cap culture tube. Dilute to a final concentration of 50-300 µg/ml in the appropriate serum supplemented media.Solution may be filtered on low protein-binding membranes if necessary.
    • Preparation
      0.1 micron filtered.
    • Source
      Purified endothelial mitogen from bovine hypothalamus.
    • Preservative Stabilisers
      See Buffer solution.
    • Purity
      Microorganisms - not detected.
      Mycoplasma - not detected.
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline, pH 7.4
    • Storage
      Lyophilised product is stable for 6 months at 4ºC.
      Solutions are stable for 2 weeks at 4ºC or 1 month at -20ºC. Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.
    • Shelf Life
      6 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Tissue Culture

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional ECGS Formats

    Formats Applications Sizes available
    ECGS : Purified TC 50 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse Monoclonal Antibody Isotyping Test KitMMT110 TestsIS
          Rat Monoclonal Antibody Isotyping Test KitRMT110 TestsIS
          Mycoplasma Removal AgentBUF0355 mlTC

          Recommended Positive Controls

            Histology Controls

                • Product Images

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