IgY Antibody | 16C7

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  • Mouse anti Duck IgY Heavy Chain
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    16C7
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2481E, F, WBdatasheet pdfdatasheet pdf0.25 mg
    MCA2481
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • MCA2481 recognises both the IgY and IgY(-Fc) Pekin duck heavy chain molecules.

      The duck IgY molecule, consisting of two heavy (H) and two light (L) chains, has been categorized as the 7.8S full-length IgY form and the 5.7S truncated IgY(-Fc) form. Studies have shown that the IgY(-Fc) molecule possesses the H chain Cu1 and Cu2 constant domains, but is lacking the Cu3 and Cu4 constant region terminal domains, all four of which are present in the full-length IgY molecule. Thus the structure of the IgY(-Fc) molecule resembles that of a F(ab’)2 fragment of IgY.

      MCA2481 also detects IgY heavy chain in Mallard ducks, goose and swan.

      MCA2481 detects bands of approximately 118kDa and 178-200kDa in Pekin duck cell lysates under non-reducing conditions in Western blotting.
    • Intended Use
    • Target Species
      Duck
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Chickenno
      Gooseyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide (NaN3)
    • Immunogen
      Purified Pekin duck yolk IgY.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised Balb/c mice were fused with cells of the SP2/0 mouse myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Flow Cytometry
      Western Blottingnon-reducing

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional IgY Antibody Formats

    Formats Clone Applications Sizes available
    IgY Antibody : Purified 16C7 E, F, WB 0.25 mg
    • Copyright © 2016 Bio-Rad

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      STAR117F
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      STAR117P
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      STAR13B

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Further Reading

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