Recombinant Dog TNF alpha (PDP018) detected using Goat anti Dog TNF alpha (AHP2975 ) as the capture reagent and Goat anti Dog TNF alpha:Biotin (AHP2975B ) as the detection reagent followed by Streptavidin:HRP
Recombinant Dog tumor necrosis factor alpha (TNF alpha), also known as tumor necrosis factor ligand superfamily member 2 (TNFSF2), is a recombinant protein corresponding to a 157 amino acid sequence of native canine TNF alpha. This recombinant protein is produced in yeast cells and is naturally folded and post translationally modified.
Canine TNF alpha is produced by a range of cells, including macrophages, monocytes, T and B lymphocytes and is an important mediator of the canine inflammatory response.
This product may be used as a standard in immunoassays.
Purified recombinant protein - lyophilized
Reconstitute with 20 µl sterile PBS containing 0.1% carrier protein Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Recombinant protein expressed in Pichia pastoris and purified by ion-exchange chromatography
>95% by SDS PAGE analysis
Approx. Protein Concentrations
Protein Molecular Weight
Reagents In The Kit
Preparing The Antibody
Phosphate buffered saline
Prior to reconstitution store at -20oC. After reconstitution store at -20oC. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
This reagent may be used as a standard in a sandwich ELISA assay in conjunction with AHP2975 as a coating antibody and AHP2975B as a detection antibody.