TNF Alpha

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Dog TNF alpha ELISA

Recombinant Dog TNF alpha (PDP018) detected using Goat anti Dog TNF alpha (AHP2975 ) as the capture reagent and Goat anti Dog TNF alpha:Biotin (AHP2975B ) as the detection reagent followed by Streptavidin:HRP

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  • Recombinant Dog TNF Alpha
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  • Product Type
    Recombinant Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    PDP018E, WBdatasheet pdfdatasheet pdf5 µg
    PDP018
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Recombinant Dog tumor necrosis factor alpha (TNF alpha), also known as tumor necrosis factor ligand superfamily member 2 (TNFSF2), is a recombinant protein corresponding to a 157 amino acid sequence of native canine TNF alpha. This recombinant protein is produced in yeast cells and is naturally folded and post translationally modified.

      Canine TNF alpha is produced by a range of cells, including macrophages, monocytes, T and B lymphocytes and is an important mediator of the canine inflammatory response.

      This product may be used as a standard in immunoassays.
    • Intended Use
    • Target Species
      Dog
    • Product Form
      Purified recombinant protein - lyophilized
    • Reconstitution
      Reconstitute with 20 µl sterile PBS containing 0.1% carrier protein
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
    • Preparation
      Recombinant protein expressed in Pichia pastoris and purified by ion-exchange chromatography
    • Preservative Stabilisers
      10% Trehalose
    • Purity
      >95% by SDS PAGE analysis
    • Approx. Protein Concentrations
    • Protein Molecular Weight
      16.9 kDa
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Prior to reconstitution store at -20oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.
    • Shelf Life
      12 months from date of despatch
    • GO Terms
      cytokine activity
      extracellular space
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Western Blotting

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
      This reagent may be used as a standard in a sandwich ELISA assay in conjunction with AHP2975 as a coating antibody and AHP2975B as a detection antibody.
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional TNF Alpha Formats

    Formats Applications Sizes available
    TNF Alpha : Rec. Protein E, WB 5 µg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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