Luteinizing Hormone Antibody

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  • Rabbit anti Dog Luteinizing Hormone
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    5720-5609C, WBdatasheet pdfdatasheet pdf50 µl
    5720-5609
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rabbit anti Dog luteinizing hormone polyclonal antibody was raised in rabbits against highly purified canine luteinizing hormone. In a simple radioimmune assay with iodinated tracers, the antibody was shown to be specific for canine luteinizing hormone (LH), also known as lutropin and does not react with canine growth hormone (GH), follicle stimulating hormone (FSH), prolactin or thyroid stimulating hormone (TSH).

      Luteinizing hormone is one of the four major glycoprotein hormones and consists of non-covalently linked alpha and beta chains.

      Rabbit anti dog luteinizing hormone antibody was raised against the intact hormone but does not cross react with the other major glycoprotein hormones which also contain the common the α chain. Rabbit anti dog luteinizing hormone is expected to show principal reactivity with the LH specific β chain and possibly with additional β chain conformational epitopes dependent on the presence of the shared α chain.
    • Intended Use
    • Target Species
      Dog
    • Product Form
      Ig fraction - lyophilised
    • Reconstitution
      Reconstitute with 50 µl of sterile distilled water. For long term storage the addition of a preservative is recommended.
    • Preparation
      Ig fraction prepared by ammonium sulphate precipitation
    • Preservative Stabilisers
      None present
    • Immunogen
      Purified canine luteinizing hormone
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Ammonium bicarbonate
    • Storage
      Prior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution.
    • GO Terms
      extracellular region
      hormone activity
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen1/2001/500
      Western Blotting1/3001/400

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Luteinizing Hormone Antibody Formats

    Formats Applications Sizes available
    Luteinizing Hormone Antibody : Purified C, WB 50 µl
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (Fc):BiotinSTAR121B1 mgE, WB
      STAR121B
      Sheep anti Rabbit IgG:Biotin2AB02B1 mlC, E, P, WB
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      Sheep anti Rabbit IgG:DyLight®488STAR36D488GA0.1 mgF, IF
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      Sheep anti Rabbit IgG:Dylight®800STAR36D800GA0.1 mgF, IF, WB
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      Goat anti Rabbit IgG (Fc):FITCSTAR121F1 mgF
      STAR121F
      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
      STAR34B
      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      STAR121P
      Goat anti Rabbit IgG (H/L):HRPSTAR124P1 mgC, E, WB
      STAR124P
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      STAR54
      Sheep anti Rabbit IgG:RPESTAR35A1 mlF
      STAR35A

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          TidyBlot Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          STAR209P

          Recommended Positive Controls

            Histology Controls

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