IL-1RA Antibody

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Dog Interleukin-1RA ELISA

Recombinant Dog interleukin-1RA (PDP012 ) detected using Goat anti Dog interleukin-1RA (AHP2979 ) as the capture reagent and Goat anti Dog interleukin-1RA:Biotin (AHP2979B ) as the detection reagent followed by Streptavidin:HRP

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  • IL-1RA Antibody thumbnail image 1
  • Goat anti Dog Interleukin-1RA
  • Goat anti Dog Interleukin-1RA:Biotin
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP2979E, WBdatasheet pdfdatasheet pdf0.1 mg
    AHP2979
    AHP2979BEdatasheet pdfdatasheet pdf50 µg
    AHP2979B
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Goat anti Dog interleukin-1RA antibody recognizes canine interleukin-1 receptor antagonist, a 16.7 kDa protein belonging to the IL-1 cytokine family.

      Canine IL-1RA is secreted by several cell types, including epithelial cells and adipocytes. IL-1RA functions by inhibiting the pro-inflammatory effects of both IL-1α and IL-1β.
    • Intended Use
    • Target Species
      Dog
    • Product Form
      Purified IgG - liquid
      Purified IgG conjugated to Biotin - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to canine interleukin-1RA were raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Recombinant canine interleukin-1RA
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch
      12 months from date of despatch
    • GO Terms
      interleukin-1 receptor antagonist activity
      extracellular space
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.5ug/ml5ug/ml
      Western Blotting1ug/ml10ug/ml
    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.01ug/ml0.2ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
      This product may be used as a capture antibody in a sandwich ELISA together with AHP2979B as the detection reagent. Recombinant Dog interleukin-1RA (PDP012 ) may be used as a standard.
    • ELISA
      This product may be used as a detection antibody in a sandwich ELISA together with AHP2979 as the capture reagent. Recombinant Dog interleukin-1RA (PDP012 ) may be used as a standard.
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional IL-1RA Antibody Formats

    Formats Applications Sizes available
    IL-1RA Antibody : Biotin E 50 µg
    IL-1RA Antibody : Purified E, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Goat IgG (Fc):FITCSTAR122F1 mgF
      STAR122F
      Rabbit anti Goat IgG (Fc):HRPSTAR122P1 mgC, E, WB
      STAR122P
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Streptavidin:APCSTAR119100 TestsF
      STAR119

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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