IL-1 Beta Antibody

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IL-1 Beta Antibody gallery image 1

Dog Interleukin-1 beta ELISA

Recombinant Dog interleukin-1 beta (PDP014 ) detected using Rabbit anti Dog interleukin-1 beta (AHP2980 ) as the capture reagent and Rabbit anti Dog interleukin-1 beta:Biotin (AHP2980B ) as the detection reagent followed by Streptavidin:HRP

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  • Rabbit anti Dog Interleukin-1 beta:Biotin
  • Rabbit anti Dog Interleukin-1 beta
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP2980E, WBdatasheet pdfdatasheet pdf0.1 mg
    AHP2980
    AHP2980BEdatasheet pdfdatasheet pdf50 µg
    AHP2980B
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rabbit anti Dog interleukin-1 beta antibody recognizes canine interleukin-1 beta (IL-1β), a 17.5 kDa pleiotropic pro-inflammatory cytokine belonging to the IL-1 superfamily. It is secreted by activated macrophages and monocytes and is involved in the modulation of a wide range of immune and inflammatory responses.

      IL-1β and the closely related IL-1α, are both involved in haematopoiesis and exhibit many identical immunomodulatory properties, binding to the same type I interleukin-1 receptor, also known as CD121a.
    • Intended Use
    • Target Species
      Dog
    • Product Form
      Purified IgG conjugated to Biotin - liquid
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to canine interleukin-1 beta were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Recombinant canine interleukin-1 beta
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch
      12 months from date of despatch
    • GO Terms
      growth factor activity
      fever generation
      positive regulation of cell division
      immune response
      extracellular space
      interleukin-1 receptor binding
      cytokine activity
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.1ug/ml0.5ug/ml
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1ug/ml5ug/ml
      Western Blotting0.1ug/ml2ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
      This product may be used as a detection antibody in a sandwich ELISA together with AHP2980 as the capture reagent. Recombinant Dog interleukin-1 beta (PDP014 ) may be used as a standard.
    • ELISA
      This product may be used as a capture antibody in a sandwich ELISA together with AHP2980B as the detection reagent. Recombinant Dog interleukin-1 beta (PDP014 ) may be used as a standard.
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional IL-1 Beta Antibody Formats

    Formats Applications Sizes available
    IL-1 Beta Antibody : Purified E, WB 0.1 mg
    IL-1 Beta Antibody : Biotin E 50 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Streptavidin:APCSTAR119100 TestsF
      STAR119
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (Fc):BiotinSTAR121B1 mgE, WB
      STAR121B
      Sheep anti Rabbit IgG:Biotin2AB02B1 mlC, E, P, WB
      2AB02B
      Sheep anti Rabbit IgG:DyLight®488STAR36D488GA0.1 mgF, IF, WB
      STAR36D488GA
      Sheep anti Rabbit IgG:DyLight®549STAR36D549GA0.1 mgF, IF
      STAR36D549GA
      Sheep anti Rabbit IgG:DyLight®649STAR36D649GA0.1 mgF, IF
      STAR36D649GA
      Sheep anti Rabbit IgG:Dylight®800STAR36D800GA0.1 mgF, IF, WB
      STAR36D800GA
      Goat anti Rabbit IgG (Fc):FITCSTAR121F1 mgF
      STAR121F
      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
      STAR34B
      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      STAR121P
      Goat anti Rabbit IgG (H/L):HRPSTAR124P1 mgC, E, WB
      STAR124P
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      STAR54
      Sheep anti Rabbit IgG:RPESTAR35A1 mlF
      STAR35A

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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