IgG2 Antibody

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IgG2 Antibody gallery image 1

Published customer image:
Horseradish peroxidase conjugated Goat anti Canine IgG2 (AHP948P) antibody used to evaluate the immunoglobulin response to Leishmania infantum promastigotes by sympomatic and asymptomatic dogs by western blotting.
Image caption:
IgG reaction pattern associated with CVL status.
Total Leishmania infantum promastigote cell extracts were separated with 12% SDS-PAGE and electrotransferred to nitrocellulose membranes. The membranes were probed with serum obtained from individual asymptomatic dogs (1:500) or symptomatic dogs (1:1,000) and secondary anti-dog (A) IgG, (B) IgG1 or (C) IgG2 (1:10,000). Representative western blots from each group of dogs are depicted (asymptomatic: n = 5; symptomatic: n = 3). Numbers represent the code numbers of the dogs enrolled in the study. Molecular weight markers are shown on the left-hand side.

From: Agallou M, Athanasiou E, Samiotaki M, Panayotou G, Karagouni E (2016)
Identification of Immunoreactive Leishmania infantum Protein Antigens to Asymptomatic Dog Sera through Combined Immunoproteomics and Bioinformatics Analysis.
PLoS ONE 11(2): e0149894.

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  • IgG2 Antibody thumbnail image 1
  • Sheep anti Dog IgG2:Alk. Phos.
  • Sheep anti Dog IgG2
  • Sheep anti Dog IgG2:HRP
  • Sheep anti Dog IgG2:FITC
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
4 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP948ABEdatasheet pdfdatasheet pdf0.5 mg
    AHP948AB
    AHP948C, E, WBdatasheet pdfdatasheet pdf1 mg
    AHP948
    AHP948PC, E, WBdatasheet pdfdatasheet pdf1 mg
    AHP948P
    AHP948FFdatasheet pdfdatasheet pdf1 mg
    AHP948F
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Sheep anti Dog IgG2 polyclonal antibody specifically recognizes canine IgG2 by electrophoresis and ELISA. No cross reactivity was detected against other canine immunoglobulin classes, isolated immunoglobulin light chains or no immunoglobulin proteins.

      Goat anti Dog IgG2 may cross react with IgG2 from other species.
    • Intended Use
    • Target Species
      Dog
    • Product Form
      Purified IgG conjugated to Alkaline Phosphatase - liquid
      Purified IgG - liquid
      Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to canine IgG2 were raised by repeated immunisations of sheep with highly purified antigen. Purified IgG prepared by affinity chromatography.
    • Preservative Stabilisers
      0.09%Sodium Azide
      0.2%Bovine Serum Albumin
      0.09%Sodium Azide
      0.1% Proclin™ 300
      0.2% Bovine Serum Albumin
      0.09%Sodium Azide
      0.2%Bovine Serum Albumin
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      50mM HEPES, 0.1M NaCl, 1mM MgCl2, 0.1mM ZnCl2
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC.
      DO NOT FREEZE

      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch.
      18 months from date of despatch.
      12 months from date of despatch.
      12 months from date of despatch.
    • Acknowledgements
      Proclin™ 300 is a trademark of The Dow Chemical Company (“Dow”) or an affiliated company of Dow.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/10001/10000
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/1001/10,000
      Immunohistology - Frozen1/2001/2000
      Western Blotting1/10001/10,000
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/10,0001/100,000
      Immunohistology - Frozen1/2001/500
      Western Blotting1/10001/10,000
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/2001/2000

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional IgG2 Antibody Formats

    Formats Applications Sizes available
    IgG2 Antibody : Alk. Phos. E 0.5 mg
    IgG2 Antibody : Purified C, E, WB 1 mg
    IgG2 Antibody : HRP C, E, WB 1 mg
    IgG2 Antibody : FITC F 1 mg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Sheep IgG (H/L):Biotin5184-23041.5 mlC, E, F, WB
      5184-2304
      Donkey anti Sheep/Goat IgG:DyLight®488STAR88D488GA0.1 mgF, IF
      STAR88D488GA
      Donkey anti Sheep/Goat IgG:DyLight®549STAR88D549GA0.1 mgF, IF
      STAR88D549GA
      Donkey anti Sheep/Goat IgG:DyLight®649STAR88D649GA0.1 mgF, IF
      STAR88D649GA
      Donkey anti Sheep/Goat IgG:FITCSTAR88F1 mgC
      STAR88F
      Donkey anti Sheep/Goat IgG:HRPSTAR88P1 mlC, E, P, WB
      STAR88P

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          AbGUARD® HRP Stabilizer PlusBUF052A100 mlC, E, P, WB
          BUF052A
          TMB Signal+BUF054A100 mlE
          BUF054A
          TMB CoreBUF056A100 mlE
          BUF056A
          TMB Core PrestainedBUF057A100 mlE
          BUF057A
          TMB Core+BUF062A100 mlE
          BUF062A
          AbGUARD® HRP Stabilizer PlusBUF052C1000 mlC, E, P, WB
          BUF052C
          AbGUARD® HRP Stabilizer PlusBUF052B500 mlC, E, P, WB
          BUF052B

          Recommended Positive Controls

            Histology Controls

              References

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