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Horseradish peroxidase conjugated Goat anti Canine IgG1 (AHP947P) antibody used to evaluate the immunoglobulin response to Leishmania infantum promastigotes by sympomatic and asymptomatic dogs by western blotting.
IgG reaction pattern associated with CVL status.
Total Leishmania infantum promastigote cell extracts were separated with 12% SDS-PAGE and electrotransferred to nitrocellulose membranes. The membranes were probed with serum obtained from individual asymptomatic dogs (1:500) or symptomatic dogs (1:1,000) and secondary anti-dog (A) IgG, (B) IgG1 or (C) IgG2 (1:10,000). Representative western blots from each group of dogs are depicted (asymptomatic: n = 5; symptomatic: n = 3). Numbers represent the code numbers of the dogs enrolled in the study. Molecular weight markers are shown on the left-hand side.
From: Agallou M, Athanasiou E, Samiotaki M, Panayotou G, Karagouni E (2016)
Identification of Immunoreactive Leishmania infantum Protein Antigens to Asymptomatic Dog Sera through Combined Immunoproteomics and Bioinformatics Analysis.
PLoS ONE 11(2): e0149894.