IgE Antibody | E6-71A1

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  • Mouse anti Dog IgE:Biotin
  • Mouse anti Dog IgE
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    E6-71A1
  • Isotype
    IgG1
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1896BE, WBdatasheet pdfdatasheet pdf0.25 mg
    MCA1896B
    MCA1896E, WBdatasheet pdfdatasheet pdf0.25 mg
    MCA1896
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Dog IgE antibody, clone E6-71A1 recognises canine IgE. E6-2A1 does not cross react with dog IgM, IgA, IgG1 or IgG2. Western blot analysis against affinity purified dog IgE using Mouse anti Dog IgE clone E6-71A1 demonstrates a single major band of 62-65kDa under reducing conditions.
    • Intended Use
    • Target Species
      Dog
    • Product Form
      Purified IgG conjugated to Biotin - liquid
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
    • Preservative Stabilisers
      0.05%Sodium Azide
      0.05% Sodium Azide (NaN3)
    • Immunogen
      Affinity purified IgE preparation from heavily parasitized and allergic dog serum.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Western Blotting
    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Western Blotting

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
      The antibody may be used as a detection antibody in a sandwich ELISA in combination with MCA1895 as the capture antibody.
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
      Under reducing conditions MCA1896B detects a band of approximately 62-65 kDa in samples of affinity purified dog IgE.
    • Western Blotting
      Under reducing conditions MCA1896 detects a band of approximately 62-65 kDa in samples of affinity purified dog IgE.
    • Instructions For Use
    • Instructions For Use

    Additional IgE Antibody Formats

    Formats Clone Applications Sizes available
    IgE Antibody : Biotin E6-71A1 E, WB 0.25 mg
    IgE Antibody : Purified E6-71A1 E, WB 0.25 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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