IgA Antibody

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IgA Antibody gallery image 1

Published customer image:
Goat anti Dog IgA antibody used for the measurement of serum IgA levels in dogs dogs with atopic dermatitis by ELISA.
Image caption:
Correlation between the phenotypes and obvious population structure was detected in the GSD population. The difference in IgA levels (pdiff = 1.1×10-5, based on Welsh two sample t-test) in CAD cases and CAD controls (Ntotal = 184, before removing CAD controls with low IgA levels) is presented with boxplots (A). The GSD population is visualised with an MDS-plot displaying the formation of two subpopulations (B) with the uneven distribution of CAD cases and CAD controls (C). The distribution of IgA levels across the subpopulations is visualized with violin plots (D). Panels B-D include all dogs after QC (n = 203) and the dotted lines on the violin plots (A and D) correspond to the respective median values.

From: Tengvall K, Kierczak M, Bergvall K, Olsson M, Frankowiack M, et al. (2013) Genome-Wide Analysis in German Shepherd Dogs Reveals Association of a Locus on CFA 27 with Atopic Dermatitis. PLoS Genet 9(5): e1003475.

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  • IgA Antibody thumbnail image 1
  • Goat anti Dog IgA:HRP
  • Goat anti Dog IgA:Alk. Phos.
  • Goat anti Dog IgA:FITC
  • Goat anti Dog IgA
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
4 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AAI31ABEdatasheet pdfdatasheet pdf0.5 mg
    AAI31AB
    AAI31PC, Edatasheet pdfdatasheet pdf1 mg
    AAI31P
    AAI31FC, Fdatasheet pdfdatasheet pdf1 mg
    AAI31F
    AAI31C, E, ID, WBdatasheet pdfdatasheet pdf1 mg
    AAI31
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Goat anti Dog IgA polyclonal antibody recognises canine IgA and shows no cross reactivity with other canine immunoglobulin classes in immunoelectrophoresis or ELISA.

      Goat anti Dog IgA may cross react with IgA from other species. This anti Dog IgA has been shown to cross react with IgA from other canids (Frankowiack et al. 2013) and with mustelids (Martel et al. 2009).
    • Intended Use
    • Target Species
      Dog
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      MustelidExpected from Sequence
      Ferretyes
      Wolfyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
      Purified IgG conjugated to Alkaline Phosphatase - liquid
      Purified IgG fraction conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG - Liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to canine IgA were raised by repeated immunisation of goat with highly purified antigen. Purified IgG prepared by affinity chromatography.
    • Preservative Stabilisers
      0.1% Proclin™ 300
      0.2% Bovine Serum Albumin
      0.09%Sodium Azide
      0.2%Bovine Serum Albumin
      0.09%Sodium Azide
      0.2%Bovine Serum Albumin
      0.09%Sodium Azide
    • Immunogen
      Purified canine IgA.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
      IgG concentration 0.5mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      50mM HEPES, 0.1M NaCl, 1mM MgCl2, 0.1mM ZnCl2
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC.
      DO NOT FREEZE.

      This product should be stored undiluted.
      Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch.
      12 months from date of despatch.
      12 months from date of despatch.
      18 months from date of despatch.
    • Acknowledgements
      Proclin™ 300 is a trademark of The Dow Chemical Company (“Dow”) or an affiliated company of Dow.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/10,0001/80,000
      Immunohistology - Frozen1/2001/500
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/10001/10000
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      Immunohistology - Frozen1/501/500
    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Immunodiffusion
      Immunohistology - Frozen1/2001/500
      Western BlottingReducing conditions

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional IgA Antibody Formats

    Formats Applications Sizes available
    IgA Antibody : FITC C, F 1 mg
    IgA Antibody : Purified C, E, ID, WB 1 mg
    IgA Antibody : Alk. Phos. E 0.5 mg
    IgA Antibody : HRP C, E 1 mg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Goat IgG (Fc):FITCSTAR122F1 mgF
      STAR122F
      Rabbit anti Goat IgG (Fc):HRPSTAR122P1 mgC, E, WB
      STAR122P

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          AbGUARD® HRP Stabilizer PlusBUF052A100 mlC, E, P, WB
          BUF052A
          TMB Signal+BUF054A100 mlE
          BUF054A
          TMB CoreBUF056A100 mlE
          BUF056A
          TMB Core PrestainedBUF057A100 mlE
          BUF057A
          TMB Core+BUF062A100 mlE
          BUF062A
          AbGUARD® HRP Stabilizer PlusBUF052C1000 mlC, E, P, WB
          BUF052C
          AbGUARD® HRP Stabilizer PlusBUF052B500 mlC, E, P, WB
          BUF052B

          Recommended Positive Controls

            Histology Controls

              References

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