IFN Gamma Antibody

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Dog Interferon gamma ELISA

Recombinant Dog interferon gamma (PDP010) detected using Rabbit anti Dog interferon gamma (AHP2977 ) as the capture reagent and Rabbit anti Dog interferon gamma:Biotin (AHP2977B ) as the detection reagent followed by Streptavidin:HRP

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  • Rabbit anti Dog Interferon Gamma
  • Rabbit anti Dog Interferon Gamma:Biotin
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP2977E, WBdatasheet pdfdatasheet pdf0.1 mg
    AHP2977
    AHP2977BEdatasheet pdfdatasheet pdf50 µg
    AHP2977B
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rabbit anti Dog interferon gamma antibody recognizes canine interferon gamma (IFNγ), a 21.4 kDa type II interferon belonging to, and being the only member of, the interferon type II family. IFNγ, produced by various cell types including T-cells and NK cells, is a cytokine involved in both innate and adaptive immunity. Canine IFNγ is predominantly secreted by activated T lymphocytes in response to viral infection and to infection by certain bacterial and protozoan pathogens.
    • Intended Use
    • Target Species
      Dog
    • Product Form
      Purified IgG - liquid
      Purified IgG conjugated to Biotin - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to canine interferon gamma were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Recombinant canine interferon gamma
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch.
      12 months from date of despatch.
    • GO Terms
      extracellular space
      response to virus
      interferon-gamma receptor binding
      cytokine activity
      immune response
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.5ug/ml5ug/ml
      Western Blotting0.1ug/ml2ug/ml
    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.05ug/ml0.5ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
      This product may be used as a capture antibody in a sandwich ELISA together with AHP2977B as the detection reagent. Recombinant Dog interferon gamma (PDP010 ) may be used as a standard.
    • ELISA
      This product may be used as a detection antibody in a sandwich ELISA together with AHP2977 as the capture reagent. Recombinant Dog interferon gamma (PDP010 ) may be used as a standard.
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional IFN Gamma Antibody Formats

    Formats Applications Sizes available
    IFN Gamma Antibody : Biotin E 50 µg
    IFN Gamma Antibody : Purified E, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (Fc):BiotinSTAR121B1 mgE, WB
      STAR121B
      Sheep anti Rabbit IgG:Biotin2AB02B1 mlC, E, P, WB
      2AB02B
      Sheep anti Rabbit IgG:DyLight®488STAR36D488GA0.1 mgF, IF, WB
      STAR36D488GA
      Sheep anti Rabbit IgG:DyLight®549STAR36D549GA0.1 mgF, IF
      STAR36D549GA
      Sheep anti Rabbit IgG:DyLight®649STAR36D649GA0.1 mgF, IF
      STAR36D649GA
      Sheep anti Rabbit IgG:Dylight®800STAR36D800GA0.1 mgF, IF, WB
      STAR36D800GA
      Goat anti Rabbit IgG (Fc):FITCSTAR121F1 mgF
      STAR121F
      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
      STAR34B
      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      STAR121P
      Goat anti Rabbit IgG (H/L):HRPSTAR124P1 mgC, E, WB
      STAR124P
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      STAR54
      Sheep anti Rabbit IgG:RPESTAR35A1 mlF
      STAR35A
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Streptavidin:APCSTAR119100 TestsF
      STAR119

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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