Collagen I/III Antibody

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  • Rabbit anti Dog Collagen I/III
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    2150-2355C, Edatasheet pdfdatasheet pdf0.5 ml
    2150-2355
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rabbit anti Dog collagen 1/III polyclonal antibody is specific for dog collagen types I and III. Type I collagen is a fibril-forming collagen found in most connective tissues and is abundant in tendons, skin, artery walls and scar tissue. Rabbit anti Dog collagen I/III also recognises collagen type III, a fibrillar collagen that is found in extendable connective tissues such as skin, lung, uterus, intestine and the vascular system and is produced by immature fibroblasts before the tougher type I.

      Rabbit anti Dog collagen I/III shows strong reactivity with both collagens I and III whilst demonstrating low reactivity with other canine collagens and serum proteins

      Dog collagen type I 100%
      Dog collagen type III 100%
      Dog collagen type II <3.0%
      Dog collagen type IV <1.0%
      Dog collagen type V <1.0%
      Dog albumin 0.5%
      Dog fibronectin 0.5%
      Dog immunoglobulins 0.5%

      Rabbit anti Dog collagen I/III also reacts with the heat denatured form of collagen I and III but does not cross-react with other dog plasma proteins.
    • Intended Use
    • Target Species
      Dog
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography
    • Antiserum Preparation
      Purified IgG was isolated by antigen (collagens types I =III) affinity chromatograpy and cross absorbtion against immobolized proteins from canine serum and collagen type IV.
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      Mannitol
      Dextran
    • Immunogen
      Collagen I and III from dog tail tendon
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.1mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      collagen
      extracellular matrix structural constituent
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/5001/1,500
      Immunohistology - Frozen1/151/25

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Collagen I/III Antibody Formats

    Formats Applications Sizes available
    Collagen I/III Antibody : Purified C, E 0.5 ml
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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