CD90 Antibody | YKIX337.217

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CD90 Antibody | YKIX337.217 gallery image 1

Staining of canine peripheral blood lymphocytes with Rat anti Dog CD90 (MCA1036GA) followed by Rabbit F(ab')2 anti Rat IgG:FITC (STAR17B)

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CD90 Antibody | YKIX337.217 gallery image 2

Figure A. FITC conjugated Mouse anti Canine CD3 (MCA1774F) and purified Rat IgG2a isotype control (MCA6005) detected with Goat anti Rat IgG2a DyLight 649 (STAR71D649). Figure B. FITC conjugated Mouse anti Canine CD3 (MCA1774F) and purified Mouse anti Canine CD90 (MCA1036GA) detected with Goat anti Rat IgG2a DyLight 649 (STAR71D649). All experiments performed on red cell lysed canine blood gated on mononuclear cells.

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CD90 Antibody | YKIX337.217 gallery image 3

Figure A. Purified Rat anti Canine CD90 (MCA1036GA) detected with Goat anti Rat IgG2a DyLight 649 (STAR71D649) and Mouse IgG1 FITC isotype control (MCA928F). Figure B. Purified Rat anti Canine CD90 (MCA1036GA) detected with Goat anti Rat IgG2a PE (STAR71D649) and Mouse anti Canine CD3 FITC (MCA1774F). All experiments performed on red cell lysed canine blood gated on mononuclear cells.

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  • Rat anti Dog CD90
  • Rat anti Dog CD90
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    YKIX337.217
  • Isotype
    IgG2b
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1036GAF, IPdatasheet pdfdatasheet pdf0.1 mg
    MCA1036GA
    MCA1036GF, IPdatasheet pdfdatasheet pdf0.25 mg
    MCA1036G
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rat anti Dog CD90, clone YKIX337.217 recognizes the canine Thy-1 cell surface antigen, also known as CD90. CD90 is a 20-30 kDa glycoprotein expressed by peripheral T lymphocytes, monocytes, thymocytes and vascular endothelium in dogs.

      Rat anti Dog CD90, clone YKIX337.217 has been demonstrated to deplete T cells when administered in vivo (Watson et al. 1993), and to block Con A driven T cell proliferation in vitro.

      Rat anti Dog CD90, clone YKIX337.217 is a useful clone for the immunophenotyping of cells and discrimination between a wide variety of lymphoploriferative disorders in dogs (Villiers 2006).
    • Intended Use
    • Target Species
      Dog
    • Product Form
      Purified IgG - liquid
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Pack Size: 0.1 mgPurified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Pack Size: 0.25 mgPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Pack Size: 0.1 mgPurified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Pack Size: 0.25 mgPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
      0.09%Sodium Azide
    • Immunogen
      Con A stimulated canine T cells.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised DA rats were fused with cells of the rat Y3/Ag1.2.3 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/50
      Immunoprecipitation
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/50
      Immunoprecipitation

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional CD90 Antibody Formats

    Formats Clone Applications Sizes available
    CD90 Antibody : Purified YKIX337.217 F, IP 0.1 mg | 0.25 mg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)STAR131A1 mlC, E, P, WB
      STAR131A
      Goat anti Rat IgG:Biotin (Mouse Adsorbed)STAR131B0.5 mgC, E, IF, P, WB
      STAR131B
      Goat anti Rat IgG:DyLight®549 (Mouse Adsorbed)STAR71D549GA0.1 mgF, IF
      STAR71D549GA
      Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed)STAR71D649GA0.1 mgF, IF
      STAR71D649GA
      Rabbit F(ab')2 anti Rat IgG:Dylight®800STAR16D800GA0.1 mgF, IF, WB
      STAR16D800GA
      Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)STAR71D800GA0.1 mgF, IF, WB
      STAR71D800GA
      Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)STAR690.5 mlF
      STAR69
      Rabbit F(ab')2 anti Rat IgG:FITCSTAR17B1 mgF
      STAR17B
      Goat anti Rat IgG:HRP (Mouse Adsorbed)STAR720.5 mgC, E, P
      STAR72
      Rabbit F(ab')2 anti Rat IgG:HRPSTAR21B1 mgC, E, P, RE
      STAR21B
      Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)STAR730.5 mlF
      STAR73
      Rabbit F(ab')2 anti Rat IgG:RPESTAR20A1 mlF
      STAR20A
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)STAR131A1 mlC, E, P, WB
      STAR131A
      Goat anti Rat IgG:Biotin (Mouse Adsorbed)STAR131B0.5 mgC, E, IF, P, WB
      STAR131B
      Goat anti Rat IgG:DyLight®549 (Mouse Adsorbed)STAR71D549GA0.1 mgF, IF
      STAR71D549GA
      Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed)STAR71D649GA0.1 mgF, IF
      STAR71D649GA
      Rabbit F(ab')2 anti Rat IgG:Dylight®800STAR16D800GA0.1 mgF, IF, WB
      STAR16D800GA
      Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)STAR71D800GA0.1 mgF, IF, WB
      STAR71D800GA
      Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)STAR690.5 mlF
      STAR69
      Rabbit F(ab')2 anti Rat IgG:FITCSTAR17B1 mgF
      STAR17B
      Goat anti Rat IgG:HRP (Mouse Adsorbed)STAR720.5 mgC, E, P
      STAR72
      Rabbit F(ab')2 anti Rat IgG:HRPSTAR21B1 mgC, E, P, RE
      STAR21B
      Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)STAR730.5 mlF
      STAR73
      Rabbit F(ab')2 anti Rat IgG:RPESTAR20A1 mlF
      STAR20A

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Rat IgG2b Negative ControlMCA6006GA0.1 mgF
        MCA6006GA
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Rat IgG2b Negative ControlMCA6006GA0.1 mgF
        MCA6006GA

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Immunohistology - Frozen

              References

              Further Reading

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