CD45R Antibody | YKIX753.22.2

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CD45R Antibody | YKIX753.22.2 gallery image 1

Staining of canine peripheral blood lymphocytes with rat anti-canine CD45R:FITC (MCA1043F)

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Staining of canine peripheral blood lymphocytes with rat anti-canine CD45R:Alexa Fluor®488 (MCA1043A488)

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CD45R Antibody | YKIX753.22.2 gallery image 3

Staining of canine peripheral blood lymphocytes with rat anti-canine CD45R:Alexa Fluor®647 (MCA1043A647)

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CD45R Antibody | YKIX753.22.2 gallery image 4

Figure A. A488 conjugated Rat anti Canine CD44 (MCA1041A488) and RPE conjugated Rat IgG2a isotype control (MCA6005PE). Figure B. A488 conjugated Rat anti Canine CD44 (MCA1041A488) and RPE conjugated Mouse anti Canine CD45R (MCA1043PE). All experiments performed on red cell lysed canine blood gated on mononuclear cells.

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Figure A. RPE conjugated Rat anti Canine CD45R (MCA1043PE) and A488 conjugated Rat IgG1 isotype control (MCA6005A488). Figure B. RPE conjugated Mouse anti Canine CD45R (MCA1043PE) and A488 conjugated Mouse anti Canine CD44 (MCA1041A488). All experiments performed on red cell lysed canine blood gated on mononuclear cells.

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  • Rat anti Dog CD45R:Alexa Fluor®647
  • Rat anti Dog CD45R:Alexa Fluor®488
  • Rat anti Dog CD45R:RPE
  • Rat anti Dog CD45R:FITC
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  • Product Type
    Monoclonal Antibody
  • Clone
    YKIX753.22.2
  • Isotype
    IgG2b
4 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1043FFdatasheet pdfdatasheet pdf0.1 mg
    MCA1043F
    MCA1043PEFdatasheet pdfdatasheet pdf100 Tests
    MCA1043PE
    MCA1043A647Fdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA1043A647
    MCA1043A488Fdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA1043A488
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rat anti Dog CD45R antibody, clone YKIX753.22.2 recognizes canine CD45R and was clustered at the First Canine Leucocyte Antigen Workshop (CLAW). It precipitates an antigen of 220kD from Con A Blasts, (Cobbold et al. 1994) . It is expressed on subsets of leucocytes in canine blood of all out bred mongrels and beagles tested. Rat anti dog CD45R, clone YKIX753.22.2 binds to antigen immunoprecipitated by the Rat anti Dog CD45 antibody clone YKIX716.13 (Cobbold et al. 1994)
    • Intended Use
    • Target Species
      Dog
    • Product Form
      Purified IgG conjugated to Alexa Fluor 647 - liquid
      Purified IgG conjugated to Alexa Fluor 488 - liquid
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
    • Reconstitution
      Reconstitute with 1ml distilled water
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
      0.09%Sodium Azide
      1%Bovine Serum Albumin
    • Immunogen
      Canine thymocytes
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.05mg/ml
      IgG concentration 0.05mg/ml
      IgG concentration 0.1 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised DA rat were fused with cells of the Y3/A1.2.3 rat myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      12 months from date of reconstitution.
      18 months from date of despatch.
    • Acknowledgements
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/5
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/5
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/5

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own systems with appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD45R Antibody Formats

    Formats Clone Applications Sizes available
    CD45R Antibody : FITC YKIX753.22.2 F 0.1 mg
    CD45R Antibody : Alexa Fluor® 488 YKIX753.22.2 F 100 Tests/1ml
    CD45R Antibody : Alexa Fluor® 647 YKIX753.22.2 F 100 Tests/1ml
    CD45R Antibody : RPE YKIX753.22.2 F 100 Tests
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Rat IgG2b Negative Control:Alexa Fluor® 647MCA1125A647100 Tests/1mlF
        MCA1125A647
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Rat IgG2b Negative Control:RPEMCA6006PE100 TestsF
        MCA6006PE
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Rat IgG2b Negative Control:FITCMCA6006F0.1 mgF
        MCA6006F

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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