CD4 Antibody | YKIX302.9

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CD4 Antibody | YKIX302.9 gallery image 1

Staining of canine peripheral blood lymphocytes with Rat anti Canine CD4:FITC (MCA1038F)

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CD4 Antibody | YKIX302.9 gallery image 2

Staining of canine peripheral blood lymphocytes with Rat anti Canine CD4:Alexa Fluor® 488 (MCA1038A488)

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Staining of canine peripheral blood lymphocytes with Rat anti Canine CD4:APC (MCA1038APC)

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CD4 Antibody | YKIX302.9 gallery image 4

Published customer image:
CD11b+CD14-MHCII- cells suppress T cell proliferation. Facs sorted CD11b+CD14-MHCII- cells isolated from a dog with osteosarcoma or healthy PBMCs were co-incubated with mitogen-stimulated CD4+ and CD8+ T cells isolated from a healthy dog for 72 hs. No stimulated cells were used as negative control. Proliferative responses were measured by 3H-thymidine incorporation from experiments performed in triplicate. CPM, counts per minute. Mean ± SEM are shown.

From: Goulart MR, Pluhar GE, Ohlfest JR (2012) Identification of Myeloid Derived Suppressor Cells in Dogs with Naturally Occurring Cancer. PLoS ONE 7(3): e33274.

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CD4 Antibody | YKIX302.9 gallery image 5

Published customer image:
Immunophenotypic profile of tumor infiltrating lymphocyte in canine mammary carcinomas. Analysis of tumor infiltrating T-cells, B-lymphocytes and T-cell subsets from MC-BMT or MC (A), further subcategorized according to the absence (-) or presence (+) of lymph node metastasis (-) (B). Lymphocyte populations and subsets were identified by flow cytometric immunostaining as described in Material and Methods. Data were expressed as percentage of positive cells within gated lymphocytes and CD4+/CD8+ T-cell ratio. Significant differences at p < 0.05 are highlighted by asterisk.

From: Estrela-Lima et al. BMC Cancer 2010 10:256.

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CD4 Antibody | YKIX302.9 gallery image 6

Published customer image:
Survival rates of animals with canine mammary carcinoma. Kaplan-Meier survival curves for animals for All (MC-BMT and MC) animals categorized according to the relative percentage of CD4+ T-cells (<66.7% or = 66.7%) and CD8+ T-cells (= 33.3% or >33.3%) (A). Animals were submitted to quarterly follow-ups during twelve months and survival rates expressed in days between the surgical excisions of the end of follow-up as described in Methods. The survival curves were estimated with the Kaplan-Meier method followed by Log-rank test. Correlation analysis highlighted the significant association between the percentages of CD4+ T-cells and CD8+ T-cells with the animal survival in days (B).

From: Estrela-Lima et al. BMC Cancer 2010 10:256.

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CD4 Antibody | YKIX302.9 gallery image 7

Published customer image:
Rat anti Dog CD4 antibody, clone YKIX302.9 used for the identification of CD4 expressing dog lymphocytes by flow cytometry.
Image caption:
Representative dot plots illustrating the analysis of intracellular cytokine profile in T-cell subsets. (A) Pseudocolor plot distribution of short-term in vitro cultured (control or SLA-Ag stimulated) canine whole blood sample according to cell size (Forward scatter - FSC) and granularity (Side scatter- SSC) used for lymphocyte gate selection (R1) of FSCLowSSCLow events. (B) Pseudocolor plots representing cytokines?+?(IL-17, TNF-a, IFN-?, TGF-ß and IL-4) CD4+ cells within gated lymphocytes and (C) Pseudocolor plots representing cytokines?+?(IL-17, TNF-a, IFN-?, TGF-ß and IL-4) CD8+ cells within gated lymphocytes. The frequency of cytokines+ T-cells subsets were calculated by quadrant statistics approach and first reported as percentage of gated lymphocytes prior to the calculation of the SLAg/Control indexes.

From: Costa-Pereira C, Moreira ML, Soares RP, Marteleto BH, Ribeiro VM, França-Dias MH, Cardoso LM, Viana KF, Giunchetti RC, Martins-Filho OA, Araújo MS. One-year timeline kinetics of cytokine-mediated cellular immunity in dogs vaccinated against visceral leishmaniasis. BMC Vet Res. 2015 Apr 11;11(1):92.

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CD4 Antibody | YKIX302.9 gallery image 8

Staining of canine peripheral blood lymphocytes with Rat anti Canine CD4:RPE-Cy7 (MCA1038PECY7)

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Figure A. FITC conjugated Mouse anti Canine CD3 (MCA1774F) and RPE conjugated Rat IgG2a isotype control (MCA1212PE). Figure B. FITC conjugated Mouse anti Canine CD3 (MCA1774F) and RPE conjugated Mouse anti Canine CD4 (MCA1038PE). All experiments performed on red cell lysed canine blood gated on mononuclear cells.

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CD4 Antibody | YKIX302.9 gallery image 10

Figure A. RPE conjugated Mouse anti Canine CD4 (MCA1038PE) and FITC conjugated Mouse IgG1 isotype control (MCA928F). Figure B. RPE conjugated Mouse anti Canine CD4 (MCA1038PE) and FITC conjugated Mouse anti Canine CD3 (MCA1774F). All experiments performed on red cell lysed canine blood gated on mononuclear cells.

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CD4 Antibody | YKIX302.9 gallery image 11

Figure A. A647 conjugated Mouse anti Canine CD4 (MCA1038A647) and RPE conjugated Rat IgG2a isotype control (MCA6005PE). Figure B. A647 conjugated Mouse anti Canine CD4 (MCA1038A647) and RPE conjugated Mouse anti Canine CD5 (MCA1037PE). All experiments performed on red cell lysed canine blood gated on mononuclear cells.

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CD4 Antibody | YKIX302.9 gallery image 12

Figure A. RPE conjugated Mouse anti Canine CD5 (MCA1037PE) and A647 conjugated Rat IgG2a isotype control (MCA1212A647). Figure B. RPE conjugated Mouse anti Canine CD5 (MCA1037PE) and A647 conjugated Mouse anti Canine CD4 (MCA1038A647). All experiments performed on red cell lysed canine blood gated on mononuclear cells.

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CD4 Antibody | YKIX302.9 gallery image 13

Figure A. A488 conjugated Mouse anti Canine CD4 (MCA1038A488) and purified Mouse IgG1 isotype control (MCA928) detected with Goat anti Mouse IgG1 DyLight 649 (STAR74D649). Figure B. A488 conjugated Mouse anti Canine CD4 (MCA1038A488) and purified Mouse anti Canine CD8b (MCA1775S) detected with Goat anti Mouse IgG1 DyLight 649 (STAR74D649). All experiments performed on red cell lysed canine blood gated on mononuclear cells.

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CD4 Antibody | YKIX302.9 gallery image 14

Figure A. Purified Mouse anti Canine CD8b (MCA1775S) detected with Goat anti Mouse IgG1 DyLight 649 (STAR74D649) and Rat IgG2b FITC isotype control (MCA6006F). Figure B. Purified Mouse anti Canine CD8b (MCA1775S) detected with Goat anti Mouse IgG1 PE (STAR74D649) and Rat anti Canine CD4 FITC (MCA1038F). All experiments performed on red cell lysed canine blood gated on mononuclear cells.

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  • Rat anti Dog CD4:Alexa Fluor® 488
  • Rat anti Dog CD4:Alexa Fluor® 647
  • Rat anti Dog CD4:Pacific Blue®
  • Rat anti Dog CD4
  • Rat anti Dog CD4:RPE-Cy7
  • Rat anti Dog CD4:APC
  • Rat anti Dog CD4:FITC
  • Rat anti Dog CD4:RPE
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    YKIX302.9
  • Isotype
    IgG2a
8 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1038GAC, Fdatasheet pdfdatasheet pdf0.1 mg
    MCA1038GA
    MCA1038APCFdatasheet pdfdatasheet pdf100 Tests
    MCA1038APC
    MCA1038FFdatasheet pdfdatasheet pdf100 Tests
    MCA1038F
    MCA1038A488Fdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA1038A488
    MCA1038A647Fdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA1038A647
    MCA1038PBFdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA1038PB
    MCA1038PECY7Fdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA1038PECY7
    MCA1038PEFdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA1038PE
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rat anti Dog CD4 antibody, clone YKIX302.9, is a monoclonal antibody specific for the canine CD4 cell surface antigen. Clone YKIX302.9 was clustered at the first Canine Leukocyte Antigen Workshop (CLAW) [Cobbold et al. 1992] along with clone CA13.1E4.

      Rat anti Dog CD4 (YKIX302.9) has been demonstrated to partially deplete circulating T lymphocytes when administered in vivo, but alone was not sufficient to prolong allograft survival in a canine transplant model (Watson et al. 1993).

      Uniquely amongst mammals, canine CD4 is expressed by neutrophils as well as by lymphocytes subsets Moore et al. 1992. Rat anti Canine CD4 (YKIX302.9) forms part of a panel of anti canine monoclonal antibodies used extensively in the evaluation of leukemic status in dogs (Villiers 2002).
    • Intended Use
    • Target Species
      Dog
    • Product Form
      Purified IgG conjugated to Alexa Fluor® 488 - liquid
      Purified IgG conjugated to Alexa Fluor® 647 - liquid
      Purified IgG conjugated to Pacific Blue® - liquid
      Purified IgG - liquid
      Purified IgG conjugated to R. Phycoerythrin (RPE)-Cy7 - lyophilised
      Purified IgG conjugated to Allophycocyanin (APC) - lyophilised
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
    • Reconstitution
      Reconstitute with 1ml distilled water
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
      Reconstitute with 1.0 ml distilled water
      Reconstitute with 1.0 ml distilled water
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
    • Preservative Stabilisers
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09% Sodium Azide (NaN3)
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      5% Sucrose
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
    • Immunogen
      Canine concanavilin A activated T cell blasts.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.05 mg/ml
      IgG concentration 0.05 mg/ml
      IgG concentration 0.05 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 0.1 mg/ml
      IgG concentration 0.1 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised DA rats were fused with cells of the Y3/Ag1.2.3 rat myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC.
      After reconstitution store at +4oC.
      DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light.
      Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      12 months from date of reconstitution
      18 months from date of reconstitution.
      18 months from date of despatch.
      12 months from date of reconstitution.
    • GO Terms
    • UniProt
    • Entrez Gene
    • Acknowledgements
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
      Cy® and CyDye® are registered trademarks of GE Healthcare
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/5
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/501/100
      Immunohistology - Frozen
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD4 Antibody Formats

    Formats Clone Applications Sizes available
    CD4 Antibody : Alexa Fluor® 488 YKIX302.9 F 100 Tests/1ml
    CD4 Antibody : Purified YKIX302.9 C, F 0.1 mg
    CD4 Antibody : RPE YKIX302.9 F 100 Tests/1ml
    CD4 Antibody : APC YKIX302.9 F 100 Tests
    CD4 Antibody : RPE-Cy7 YKIX302.9 F 100 Tests/1ml
    CD4 Antibody : Pacific Blue® YKIX302.9 F 100 Tests/1ml
    CD4 Antibody : Alexa Fluor® 647 YKIX302.9 F 100 Tests/1ml
    CD4 Antibody : FITC YKIX302.9 F 100 Tests
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)STAR131A1 mlC, E, P, WB
      STAR131A
      Goat anti Rat IgG:Biotin (Mouse Adsorbed)STAR131B0.5 mgC, E, IF, P, WB
      STAR131B
      Goat anti Rat IgG:DyLight®549 (Mouse Adsorbed)STAR71D549GA0.1 mgF, IF
      STAR71D549GA
      Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed)STAR71D649GA0.1 mgF, IF
      STAR71D649GA
      Rabbit F(ab')2 anti Rat IgG:Dylight®800STAR16D800GA0.1 mgF, IF, WB
      STAR16D800GA
      Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)STAR71D800GA0.1 mgF, IF, WB
      STAR71D800GA
      Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)STAR690.5 mlF
      STAR69
      Rabbit F(ab')2 anti Rat IgG:FITCSTAR17B1 mgF
      STAR17B
      Goat anti Rat IgG:HRP (Mouse Adsorbed)STAR720.5 mgC, E, P
      STAR72
      Rabbit F(ab')2 anti Rat IgG:HRPSTAR21B1 mgC, E, P, RE
      STAR21B
      Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)STAR730.5 mlF
      STAR73
      Rabbit F(ab')2 anti Rat IgG:RPESTAR20A1 mlF
      STAR20A

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Rat IgG2a Negative Control:Alexa Fluor® 488MCA1212A488100 Tests/1mlF
        MCA1212A488
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Rat IgG2a Negative Control:Alexa Fluor® 647MCA1212A647100 Tests/1mlF
        MCA1212A647
        Rat IgG2a Negative Control:Alexa Fluor® 647MCA6005A647100 Tests/1mlF
        MCA6005A647
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Rat IgG2a Negative Control:Pacific Blue®MCA1212PB100 Tests/1mlF
        MCA1212PB
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Rat IgG2a Negative Control:RPE-Cy7MCA6005PECY7100 Tests/1mlF
        MCA6005PECY7
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Rat IgG2a Negative Control:APCMCA6005APC100 TestsF
        MCA6005APC
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Rat IgG2a Negative Control:FITCMCA6005F0.1 mgF
        MCA6005F
        Rat IgG2a Negative Control:FITCMCA1212F100 TestsF
        MCA1212F
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Rat IgG2a Negative Control:RPEMCA1212PE100 TestsF
        MCA1212PE
        Rat IgG2a Negative Control:RPEMCA6005PE100 TestsF
        MCA6005PE

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Flow Cytometry

              References

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