CD3/CD8 | CA17.2A12/YCATE55.9

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CD3/CD8 | CA17.2A12/YCATE55.9 gallery image 1

FITC conjugated Mouse anti Canine CD3 and PE conjugated Rat anti Canine CD8 (DC047). All experiments performed on red cell lysed canine blood gated on mononuclear cells.

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  • anti Dog CD3:FITC/CD8:RPE
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  • Product Type
    Dual Color Reagent
  • Clone
    CA17.2A12/YCATE55.9
  • Isotype
    Cocktail
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    DC047Fdatasheet pdfdatasheet pdf100 Tests
    DC047
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Anti Canine CD3 / CD8 dual color reagent is a dual colour reagent recognising canine CD3 and CD8 cell surface antigens. Clone CA17.2A12 recognises canine CD3, a T cell differentiation antigen expressed on mature T lymphocytes.

      Clone YCATE 55.9 recognises canine CD8, which is expressed by cytotoxic T cells. YCATE 55.9 binds to CD8 alpha.
    • Intended Use
    • Target Species
      Dog
    • Product Form
      Dual Colour combination consisting of FITC conjugated and RPE conjugated monoclonal antibodies mixed in optimal ratio - lyophilised.
    • Reconstitution
      Reconstitute with 1.0 ml distilled water
    • Preparation
    • Preservative Stabilisers
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
    • Purity
    • Approx. Protein Concentrations
    • Antibody Isotypes
      FITC reagent: IgG1 (MOUSE)
      RPE reagent: IgG1 (RAT)
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution.
    • GO Terms
      transmembrane receptor activity
      integral to membrane
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own systems using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CD3/CD8 Formats

    Formats Clone Applications Sizes available
    CD3/CD8 : FITC/RPE CA17.2A12/YCATE55.9 F 100 Tests
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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