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CD3/CD4 | CA17.2A12 / YKIX302.9

anti Dog CD3:FITC/CD4:RPE

Product Type
Dual Color Reagent
Clone
CA17.2A12 / YKIX302.9
Isotype
Cocktail
Specificity
CD3/CD4

Product Code Applications Pack Size List Price Your Price Qty
DC046
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DC046 is a dual colour reagent recognising canine CD3 and CD4 cell surface antigens. Clone CA17.2A12 recognises canine CD3, a T cell differentiation antigen expressed on mature T lymphocytes.

Clone YKIX 302.9 recognises canine CD4, which is expressed by lymphocyte subsets and canine neutrophils.

Target Species
Dog
Product Form
Dual Colour combination consisting of FITC conjugated and RPE conjugated monoclonal antibodies mixed in optimal ratio - lyophilized
Reconstitution
Reconstitute with 1.0 ml distilled water
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Antibody Isotypes
FITC reagent: IgG1 (MOUSE)
RPE reagent: IgG2a (RAT)
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
RPE 488nm laser 496 578
RPE 561nm laser 546 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own systems using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

References for CD3/CD4

  1. Sakai, M et al. (2006) Phenotypic analysis of hepatic T lymphocytes in a dog with chronic hepatitis.
    J Vet Med Sci. 68:1219-1221

RRID
AB_324350
UniProt
P27597
P33705
Entrez Gene
CD3E
CD4
GO Terms
GO:0016021 integral to membrane
GO:0004888 transmembrane receptor activity
GO:0007155 cell adhesion
GO:0006955 immune response
GO:0045058 T cell selection

DC046

157437 1802

If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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