CD3 Antibody | CA17.2A12

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CD3 Antibody | CA17.2A12 gallery image 1

Published customer image:
CD11b+CD14-MHCII- cells suppress T cell proliferation. Facs sorted CD11b+CD14-MHCII- cells isolated from a dog with osteosarcoma or healthy PBMCs were co-incubated with mitogen-stimulated CD4+ and CD8+ T cells isolated from a healthy dog for 72 hs. No stimulated cells were used as negative control. Proliferative responses were measured by 3H-thymidine incorporation from experiments performed in triplicate. CPM, counts per minute. Mean ± SEM are shown.

From: Goulart MR, Pluhar GE, Ohlfest JR (2012) Identification of Myeloid Derived Suppressor Cells in Dogs with Naturally Occurring Cancer. PLoS ONE 7(3): e33274.

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Staining of canine peripheral blood lymphocytes with Mouse anti Canine CD3 (MCA1774GA)

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Figure A. FITC conjugated Mouse anti Canine CD3 (MCA1774F) and RPE conjugated Rat IgG2a isotype control (MCA1212PE). Figure B. FITC conjugated Mouse anti Canine CD3 (MCA1774F) and RPE conjugated Mouse anti Canine CD4 (MCA1038PE). All experiments performed on red cell lysed canine blood gated on mononuclear cells.

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Figure A. RPE conjugated Mouse anti Canine CD4 (MCA1038PE) and FITC conjugated Mouse IgG1 isotype control (MCA928F). Figure B. RPE conjugated Mouse anti Canine CD4 (MCA1038PE) and FITC conjugated Mouse anti Canine CD3 (MCA1774F). All experiments performed on red cell lysed canine blood gated on mononuclear cells.

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Figure A. RPE conjugated Mouse anti Canine CD8 (MCA1039PE) and FITC conjugated Mouse IgG1 isotype control (MCA928F). Figure B. RPE conjugated Mouse anti Canine CD8 (MCA1039PE) and FITC conjugated Mouse anti Canine CD3 (MCA1774F). All experiments performed on red cell lysed canine blood gated on mononuclear cells.

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Figure A. FITC conjugated Mouse anti Canine CD3 (MCA1774F) and RPE conjugated Rat IgG1 isotype control (MCA6004PE). Figure B. FITC conjugated Mouse anti Canine CD3 (MCA1774F) and RPE conjugated Mouse anti Canine CD8 (MCA1039PE). All experiments performed on red cell lysed canine blood gated on mononuclear cells.

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Figure A. FITC conjugated Mouse anti Canine CD3 (MCA1774F) and purified Rat IgG2a isotype control (MCA6005) detected with Goat anti Rat IgG2a DyLight 649 (STAR71D649). Figure B. FITC conjugated Mouse anti Canine CD3 (MCA1774F) and purified Mouse anti Canine CD90 (MCA1036GA) detected with Goat anti Rat IgG2a DyLight 649 (STAR71D649). All experiments performed on red cell lysed canine blood gated on mononuclear cells.

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Figure A. Purified Rat anti Canine CD90 (MCA1036GA) detected with Goat anti Rat IgG2a DyLight 649 (STAR71D649) and Mouse IgG1 FITC isotype control (MCA928F). Figure B. Purified Rat anti Canine CD90 (MCA1036GA) detected with Goat anti Rat IgG2a PE (STAR71D649) and Mouse anti Canine CD3 FITC (MCA1774F). All experiments performed on red cell lysed canine blood gated on mononuclear cells.

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  • Mouse anti Dog CD3:FITC
  • Mouse anti Dog CD3
(Rated 0.0 out of 5 based on 0 customer reviews)
    CD3 antibody, clone CA17.2A12 recognizes the canine CD3 cell surface antigen expressed by thymocytes and mature T lymphocytes. Clone CA17.2A12 is a valuable flow cytometric and immunohistologic tool for canine lymphoma detection of T-cell origin.
    • Product Type
      Monoclonal Antibody
    • Clone
      CA17.2A12
    • Isotype
      IgG1
    2 Formats Available
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      MCA1774FFdatasheet pdfdatasheet pdf0.1 mg
      MCA1774F
      MCA1774GAC, F, IF, IP, P *datasheet pdfdatasheet pdf0.1 mg
      MCA1774GA
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      More Images
      References
      Reviews
      -
      • Mouse anti Dog CD3 antibody, clone CA17.2A12 recognises the canine CD3 cell surface antigen, expressed by thymocytes and mature T lymphocytes. CD3 is engaged in the surface expression of the T-cell antigen receptor (TCR) and the signal transduction pathway resulting from MHC ligand binding to the TCR. CD3 is made up of a number of invariant subchains of the immunoglobulin superfamily.

        Mouse anti Dog CD3 clone CA17.2A12 is a valuable flow cytometric and immunohistologic tool for canine lymphoma detection of T-cell origin (Miniscalco et al. 2003).
      • Intended Use
      • Target Species
        Dog
      • Product Form
        Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
        Purified IgG - liquid
      • Reconstitution
      • Preparation
        Purified IgG prepared by affinity chromatography on Protein G
        Purified IgG prepared by affinity chromatography on Protein G
      • Preservative Stabilisers
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        0.09%Sodium Azide
      • Immunogen
        Affinity enriched TCR/CD3 membrane proteins isolated from thymocytes and the T cell line CLGL-90
      • Purity
      • Approx. Protein Concentrations
        IgG concentration 0.1 mg/ml
        IgG concentration 1.0 mg/ml
      • Reagents In The Kit
      • Preparing The Antibody
      • Test Principle
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
      • Storage
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

        Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      • Shelf Life
        18 months from date of despatch.
        18 months from date of despatch.
      • GO Terms
        transmembrane receptor activity
        integral to membrane
      • UniProt
      • Entrez Gene
      • Acknowledgements
      • Regulatory
        For research purposes only
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

      • Application NameYesNoMin DilutionMax Dilution
        Flow CytometryNeat1/10
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry1/251/50
        Immunofluorescence
        Immunohistology - Frozen
        Immunoprecipitation
        Immunohistology - Paraffin(1)
        (1)
        This product requires antigen retrieval using heat treatment. Citrate buffer, pH 6.0 is recommended for this purpose.

      • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
      • Technical Advice
      • Technical Advice
      • Recommended Protocol
      • Recommended Protocol
      • Flow Cytometry
        Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
      • ELISA
      • ELISA
      • Immunohistology
      • Immunohistology
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Immunofluorescence
      • Immunofluorescence
      • Western Blotting
      • Western Blotting
      • Instructions For Use
      • Instructions For Use

      Additional CD3 Antibody Formats

      Formats Clone Applications Sizes available
      CD3 Antibody : FITC CA17.2A12 F 0.1 mg
      CD3 Antibody : Purified CA17.2A12 C, F, IF, IP, P * 0.1 mg
      • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
        STAR117A
        Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
        STAR87A
        Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
        STAR117D488GA
        Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF
        STAR117D549GA
        Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
        STAR117D649GA
        Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
        STAR117D680GA
        Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
        STAR117D800GA
        Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
        STAR8D800GA
        Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
        STAR117F
        Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
        STAR70
        Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
        STAR120F
        Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
        STAR9B
        Human anti Mouse IgG1:HRPHCA036P0.1 mgE
        HCA036P
        Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
        STAR117P
        Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
        STAR77
        Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
        STAR120P
        Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
        STAR13B
        Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
        STAR87P
        Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
        STAR12A
        Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
        STAR76

        Recommended Negative Isotype Control

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse IgG1 Negative Control:FITCMCA928F100 TestsF
          MCA928F
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse IgG1 Negative ControlMCA928100 TestsF
          MCA928

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

                • Application NameReference Images
                  Flow Cytometry
                  Immunofluorescence

                References

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