CD18 Antibody | CA1.4E9

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CD18 Antibody | CA1.4E9 gallery image 1

Dog peripheral blood monocytes stained with Mouse anti Canine CD18: Alexa Fluor®647 (MCA1780A647)

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CD18 Antibody | CA1.4E9 gallery image 2

Dog peripheral blood monocytes stained with Mouse anti Dog CD18 (MCA1780GA) followed by FITC conjugated Rabbit F(ab')2 anti Mouse IgG(STAR9B)

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CD18 Antibody | CA1.4E9 gallery image 3

Published customer image:
Mouse anti Dog CD18 antibody, clone CA1.4E9 (MCA1780GA) used for the evaluation of CD18 expression on alveolar macrophages from hooded seal (Cystophora cristata) using flow cytometry.
Image caption:
Expression of membrane markers. The expression of CD14 and CD18 on hooded seal PBMC and BAL cells by flow cytometric analysis. PBMCs (left) were divided into monocyte and lymphocyte gates, while BAL cells (right) were broadly gated to encompass all cells of likely macrophage appearance. Only cells classified as viable (using Live/Dead fixable far red stain) were routed to analysis. The respective cell populations were analyzed for CD14 and CD18 (dark grey) or unstained cells (open histograms). For CD18 an indirect staining technique was used, and secondary antibodies alone are depicted in light grey histograms; the resulting dimly positive staining were assumed to be due to unspecific binding, possibly due to Fc receptor cross-reactivity (CD14; cluster of differentiation 14, CD18; cluster of differentiation 18, PBMC; peripheral blood mononuclear cell).

From: Citation: Larsen AK, Nymo IH, Boysen P, Tryland M, Godfroid J (2013) Entry and Elimination of Marine Mammal Brucella spp. by Hooded Seal (Cystophora cristata) Alveolar Macrophages In Vitro.
PLoS ONE 8(7): e70186.

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CD18 Antibody | CA1.4E9 gallery image 4

Published customer image:
Mouse anti Dog CD18 antibody, clone CA1.4E9 (MCA1780GA) used for the evaluation of CD18 expression on alveolar macrophages from hooded seal (Cystophora cristata) using immunofluorescence microscopy.
Image caption:
Macrophage identification. Adherent BAL cells cultured for 5–7 days were examined for the expression of membrane markers by the use of mAbs. Representative confocal micrographs showing BAL cells immunostained for CD14 (red) and CD18 (green). Nuclei are visualized by DRAQ5 (blue). Pictures are taken of wet samples using a 40X 1.2NA water immersion lens (mAbs; monoclonal antibodies).

From: Citation: Larsen AK, Nymo IH, Boysen P, Tryland M, Godfroid J (2013) Entry and Elimination of Marine Mammal Brucella spp. by Hooded Seal (Cystophora cristata) Alveolar Macrophages In Vitro.
PLoS ONE 8(7): e70186.

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CD18 Antibody | CA1.4E9 gallery image 5

Figure A. A647 conjugated Mouse anti Canine CD18 (MCA1780A647) and RPE conjugated Mouse IgG1 isotype control (MCA928PE). Figure B. A647 conjugated Mouse anti Canine CD18 (MCA1780A647) and RPE conjugated Mouse anti Canine CD21 (MCA1781PE). All experiments performed on red cell lysed canine blood gated on mononuclear cells.

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CD18 Antibody | CA1.4E9 gallery image 6

Figure A. RPE conjugated Mouse anti Canine CD21 (MCA1781PE) and A647 conjugated Mouse IgG1 isotype control (MCA928PE). Figure B. RPE conjugated Mouse anti Canine CD21 (MCA1781PE) and A647 conjugated Mouse anti Canine CD18 (MCA1780A647). All experiments performed on red cell lysed canine blood gated on mononuclear cells.

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  • Mouse anti Dog CD18
  • Mouse anti Dog CD18:Alexa Fluor®647
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    CA1.4E9
  • Isotype
    IgG1
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1780GAC *, F, IF, IPdatasheet pdfdatasheet pdf0.1 mg
    MCA1780GA
    MCA1780A647Fdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA1780A647
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Dog CD18 antibody, clone CA1.4E9 recognises the canine CD18 cell surface antigen, also known as the b2 integrin.

      The tissue and cellular distribution of CD18 in canine tissue closely follows that observed for humans (Moore et al. 1990) CD18 is expressed by virtually all leucocytes, but more strongly upon monocytes and granulocytes than on lymphocytes. The cross reactivity patterns of Mouse anti Canine CD18, clone CA1.4E9 further indicate that the antibody clone recognizes an epitope common to a number of mammalian species.

      Immunoprecipitation experiments using detergent lysatesof iodinated peripheral blood leukocytes indicate that clone Ca1.4E9 immunoprecipitates the common 95 kDa beta 2 integrin chain (CD18) along with the non-covalently associated alpha chains at 180 kDa (CD11a ), 165 kDa (CD11b) and 150 kDa (CD11c) (Moore et al. 1990). Of note is the lack of precipitation of the integrin alpha D chain (CD11d), this is likely due to the almost complete absence of CD11d expression on peripheral blood lymphocytes, in contrast to expression in the splenic red pulp (Fry et al. 2003).

      High CD18 expression is a common feature of lymphoma and hyperplasia in dogs (Caniatti et al. 1996)
    • Intended Use
    • Target Species
      Dog
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Horseyes
      Pigyes
      Humanyes
      Bovineyes
      Catyes
      MustelidExpected from Sequence
      Minkyes
      Hooded Sealyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
      Purified IgG conjugated to Alexa Fluor 647 - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G
      Purified IgG prepared by affinity chromatography on Protein G
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
      IgG concentration 0.05 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light.
      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
    • Acknowledgements
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/251/200
      Immunofluorescence
      Immunohistology - Paraffin
      Immunoprecipitation
      Immunohistology - Frozen(1)
      (1)
      The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional CD18 Antibody Formats

    Formats Clone Applications Sizes available
    CD18 Antibody : Purified CA1.4E9 C *, F, IF, IP 0.1 mg
    CD18 Antibody : Alexa Fluor® 647 CA1.4E9 F 100 Tests/1ml
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
      STAR117A
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      STAR87A
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
      STAR117D488GA
      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF
      STAR117D549GA
      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
      STAR117D649GA
      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
      STAR117D680GA
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      STAR117D800GA
      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      STAR8D800GA
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      STAR117F
      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
      STAR70
      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
      STAR120F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      STAR9B
      Human anti Mouse IgG1:HRPHCA036P0.1 mgE
      HCA036P
      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      STAR117P
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      STAR77
      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
      STAR120P
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
      STAR13B
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      STAR87P
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      STAR12A
      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
      STAR76

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative ControlMCA928100 TestsF
        MCA928
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative Control:Alexa Fluor® 647MCA928A647100 Tests/1mlF
        MCA928A647

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Flow Cytometry
                Immunofluorescence

              References

              Further Reading

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