Figure A. FITC conjugated Rat anti Canine CD11/18 (MCA1040F) and RPE conjugated Mouse IgG1 isotype control (MCA928PE). Figure B. FITC conjugated Rat anti Canine CD11/18 (MCA1040F) and RPE conjugated Mouse anti Canine CD25 (MCA5916PE). All experiments performed on red cell lysed canine blood gated on mononuclear cells.
Figure A. PE conjugated Mouse anti Canine CD25 (MCA5916F) and A488 conjugated Rat IgG2c isotype control (MCA2879A488). Figure B. PE conjugated Mouse anti Canine CD25 (MCA5916F) and A488 conjugated Rat anti Canine CD11/18 (MCA1040PE). All experiments performed on red cell lysed canine blood gated on mononuclear cells.
Rat anti Dog CD11/CD18 antibody, clone YKIX490.6.4 recognizes the canine leukocyte cell adhesion molecule (LeuCAM) composed of the ~180 kDa CD11a chain complexed with the ~95 kDa common beta chain CD18. The CD11a/CD18 complex is also known as LFA-1.
Rat anti Dog CD11/CD18 has been reported to block T cell proliferation, cytotoxic T lymphocyte (CTL) lysis, antibody dependent cell mediated cytotoxic (ADCC) lysis and homotypic cell adhesion (Cobbold et al. 1994)
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Bovine Serum Albumin
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Reagents In The Kit
Preparing The Antibody
Phosphate buffered saline
Spleen cells from immunised DA rat were fused with cells of the Y3/A1.2.3 rat myeloma cell line.
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
18 months from date of despatch.
For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own systems with appropriate negative/positive controls.
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
1. Cobbold, S. & Metcalfe, S. (1994) Monoclonal antibodies that define canine homologues of human CD antigens: summary of the First International Canine Leukocyte Antigen Workshop (CLAW).Tissue Antigens. 43 (3): 137-54.
2. Huang, Y.C. et al. (2008) CD5-low expression lymphocytes in canine peripheral blood show characteristics of natural killer cells.J Leukoc Biol. 84: 1501-10.