CD11/CD18 Antibody | YKIX490.6.4

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CD11/CD18 Antibody | YKIX490.6.4 gallery image 1

Figure A. FITC conjugated Rat anti Canine CD11/18 (MCA1040F) and RPE conjugated Mouse IgG1 isotype control (MCA928PE). Figure B. FITC conjugated Rat anti Canine CD11/18 (MCA1040F) and RPE conjugated Mouse anti Canine CD25 (MCA5916PE). All experiments performed on red cell lysed canine blood gated on mononuclear cells.

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CD11/CD18 Antibody | YKIX490.6.4 gallery image 2

Figure A. PE conjugated Mouse anti Canine CD25 (MCA5916F) and A488 conjugated Rat IgG2c isotype control (MCA2879A488). Figure B. PE conjugated Mouse anti Canine CD25 (MCA5916F) and A488 conjugated Rat anti Canine CD11/18 (MCA1040PE). All experiments performed on red cell lysed canine blood gated on mononuclear cells.

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  • Rat anti Dog CD11/CD18:FITC
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    YKIX490.6.4
  • Isotype
    IgG2c
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1040FFdatasheet pdfdatasheet pdf0.1 mg
    MCA1040F
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rat anti Dog CD11/CD18 antibody, clone YKIX490.6.4 recognizes the canine leukocyte cell adhesion molecule (LeuCAM) composed of the ~180 kDa CD11a chain complexed with the ~95 kDa common beta chain CD18. The CD11a/CD18 complex is also known as LFA-1.

      Rat anti Dog CD11/CD18 has been reported to block T cell proliferation, cytotoxic T lymphocyte (CTL) lysis, antibody dependent cell mediated cytotoxic (ADCC) lysis and homotypic cell adhesion (Cobbold et al. 1994)
    • Intended Use
    • Target Species
      Dog
    • Product Form
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
      1%Bovine Serum Albumin
    • Immunogen
      Canine thymocytes.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.1 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised DA rat were fused with cells of the Y3/A1.2.3 rat myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/5

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own systems with appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CD11/CD18 Antibody Formats

    Formats Clone Applications Sizes available
    CD11/CD18 Antibody : FITC YKIX490.6.4 F 0.1 mg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Flow Cytometry

              References

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