Recombinant Dog C-reactive Protein is a 204 aa recombinant protein produced in a eukaryotic expression system.
C-reactive protein is an acute phase protein, produced by the liver, that is used as a marker of inflammation. Canine C-reactive protein (CRP), as with C-reactive protein in other species, forms a pentamer. Canine CRP differs from human CRP in that two of the subunits making up the pentamer are glycosylated.
This recombinant canine CRP is partially glycosylated, and while the pattern of glycosylation differs slightly from that of native canine CRP, SDS page analysis demonstrates that this recombinant canine CRP behaves in a similar way to the native protein. With similar biochemical properties to the native canine CRP, this reagent may be used as a standard in immunoassays for canine CRP.
Purified recombinant protein - lyophilised
Reconstitute with 1 ml distilled water Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Purified by affinity chromatography
Approx. Protein Concentrations
Reagents In The Kit
Preparing The Antibody
TRIS buffered saline, 2 mM CaCl2
Prior to reconstitution store at -20oC. After reconstitution store at -20oC. Storage in frost-free freezers is not recommended. This product should be stored undiluted.
12 months from date of despatch
For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.